Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes
Large dsDNA viruses are involved in the population control of many globally distributed species of eukaryotic phytoplankton and have a prominent role in bloom termination. The genus Phaeocystis (Haptophyta, Prymnesiophyceae) includes several high-biomass-forming phytoplankton species, such as Phaeoc...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696832 http://www.ncbi.nlm.nih.gov/pubmed/23754393 https://doi.org/10.1073/pnas.1303251110 |
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ftpubmed:oai:pubmedcentral.nih.gov:3696832 2023-05-15T13:31:54+02:00 Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes Santini, Sebastien Jeudy, Sandra Bartoli, Julia Poirot, Olivier Lescot, Magali Abergel, Chantal Barbe, Valérie Wommack, K. Eric Noordeloos, Anna A. M. Brussaard, Corina P. D. Claverie, Jean-Michel 2013-06-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696832 http://www.ncbi.nlm.nih.gov/pubmed/23754393 https://doi.org/10.1073/pnas.1303251110 en eng National Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696832 http://www.ncbi.nlm.nih.gov/pubmed/23754393 http://dx.doi.org/10.1073/pnas.1303251110 Biological Sciences Text 2013 ftpubmed https://doi.org/10.1073/pnas.1303251110 2013-12-29T01:27:10Z Large dsDNA viruses are involved in the population control of many globally distributed species of eukaryotic phytoplankton and have a prominent role in bloom termination. The genus Phaeocystis (Haptophyta, Prymnesiophyceae) includes several high-biomass-forming phytoplankton species, such as Phaeocystis globosa, the blooms of which occur mostly in the coastal zone of the North Atlantic and the North Sea. Here, we report the 459,984-bp-long genome sequence of P. globosa virus strain PgV-16T, encoding 434 proteins and eight tRNAs and, thus, the largest fully sequenced genome to date among viruses infecting algae. Surprisingly, PgV-16T exhibits no phylogenetic affinity with other viruses infecting microalgae (e.g., phycodnaviruses), including those infecting Emiliania huxleyi, another ubiquitous bloom-forming haptophyte. Rather, PgV-16T belongs to an emerging clade (the Megaviridae) clustering the viruses endowed with the largest known genomes, including Megavirus, Mimivirus (both infecting acanthamoeba), and a virus infecting the marine microflagellate grazer Cafeteria roenbergensis. Seventy-five percent of the best matches of PgV-16T–predicted proteins correspond to two viruses [Organic Lake phycodnavirus (OLPV)1 and OLPV2] from a hypersaline lake in Antarctica (Organic Lake), the hosts of which are unknown. As for OLPVs and other Megaviridae, the PgV-16T sequence data revealed the presence of a virophage-like genome. However, no virophage particle was detected in infected P. globosa cultures. The presence of many genes found only in Megaviridae in its genome and the presence of an associated virophage strongly suggest that PgV-16T shares a common ancestry with the largest known dsDNA viruses, the host range of which already encompasses the earliest diverging branches of domain Eukarya. Text Antarc* Antarctica North Atlantic PubMed Central (PMC) Organic Lake ENVELOPE(78.190,78.190,-68.457,-68.457) Proceedings of the National Academy of Sciences 110 26 10800 10805 |
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PubMed Central (PMC) |
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Biological Sciences |
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Biological Sciences Santini, Sebastien Jeudy, Sandra Bartoli, Julia Poirot, Olivier Lescot, Magali Abergel, Chantal Barbe, Valérie Wommack, K. Eric Noordeloos, Anna A. M. Brussaard, Corina P. D. Claverie, Jean-Michel Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes |
topic_facet |
Biological Sciences |
description |
Large dsDNA viruses are involved in the population control of many globally distributed species of eukaryotic phytoplankton and have a prominent role in bloom termination. The genus Phaeocystis (Haptophyta, Prymnesiophyceae) includes several high-biomass-forming phytoplankton species, such as Phaeocystis globosa, the blooms of which occur mostly in the coastal zone of the North Atlantic and the North Sea. Here, we report the 459,984-bp-long genome sequence of P. globosa virus strain PgV-16T, encoding 434 proteins and eight tRNAs and, thus, the largest fully sequenced genome to date among viruses infecting algae. Surprisingly, PgV-16T exhibits no phylogenetic affinity with other viruses infecting microalgae (e.g., phycodnaviruses), including those infecting Emiliania huxleyi, another ubiquitous bloom-forming haptophyte. Rather, PgV-16T belongs to an emerging clade (the Megaviridae) clustering the viruses endowed with the largest known genomes, including Megavirus, Mimivirus (both infecting acanthamoeba), and a virus infecting the marine microflagellate grazer Cafeteria roenbergensis. Seventy-five percent of the best matches of PgV-16T–predicted proteins correspond to two viruses [Organic Lake phycodnavirus (OLPV)1 and OLPV2] from a hypersaline lake in Antarctica (Organic Lake), the hosts of which are unknown. As for OLPVs and other Megaviridae, the PgV-16T sequence data revealed the presence of a virophage-like genome. However, no virophage particle was detected in infected P. globosa cultures. The presence of many genes found only in Megaviridae in its genome and the presence of an associated virophage strongly suggest that PgV-16T shares a common ancestry with the largest known dsDNA viruses, the host range of which already encompasses the earliest diverging branches of domain Eukarya. |
format |
Text |
author |
Santini, Sebastien Jeudy, Sandra Bartoli, Julia Poirot, Olivier Lescot, Magali Abergel, Chantal Barbe, Valérie Wommack, K. Eric Noordeloos, Anna A. M. Brussaard, Corina P. D. Claverie, Jean-Michel |
author_facet |
Santini, Sebastien Jeudy, Sandra Bartoli, Julia Poirot, Olivier Lescot, Magali Abergel, Chantal Barbe, Valérie Wommack, K. Eric Noordeloos, Anna A. M. Brussaard, Corina P. D. Claverie, Jean-Michel |
author_sort |
Santini, Sebastien |
title |
Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes |
title_short |
Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes |
title_full |
Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes |
title_fullStr |
Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes |
title_full_unstemmed |
Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes |
title_sort |
genome of phaeocystis globosa virus pgv-16t highlights the common ancestry of the largest known dna viruses infecting eukaryotes |
publisher |
National Academy of Sciences |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696832 http://www.ncbi.nlm.nih.gov/pubmed/23754393 https://doi.org/10.1073/pnas.1303251110 |
long_lat |
ENVELOPE(78.190,78.190,-68.457,-68.457) |
geographic |
Organic Lake |
geographic_facet |
Organic Lake |
genre |
Antarc* Antarctica North Atlantic |
genre_facet |
Antarc* Antarctica North Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696832 http://www.ncbi.nlm.nih.gov/pubmed/23754393 http://dx.doi.org/10.1073/pnas.1303251110 |
op_doi |
https://doi.org/10.1073/pnas.1303251110 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
110 |
container_issue |
26 |
container_start_page |
10800 |
op_container_end_page |
10805 |
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1766022295592108032 |