Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH
Transmembrane acid–base fluxes affect the intracellular pH and unstirred layer pH around a superfused biological preparation. In this paper the factors influencing the unstirred layer pH and its gradient are studied. An analytical expression of the unstirred layer pH gradient in steady state is deri...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689360 http://www.ncbi.nlm.nih.gov/pubmed/23860924 https://doi.org/10.1007/s10867-013-9309-9 |
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ftpubmed:oai:pubmedcentral.nih.gov:3689360 2023-05-15T15:52:47+02:00 Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH Marrannes, Roger 2013-04-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689360 http://www.ncbi.nlm.nih.gov/pubmed/23860924 https://doi.org/10.1007/s10867-013-9309-9 en eng Springer Netherlands http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23860924 http://dx.doi.org/10.1007/s10867-013-9309-9 © Springer Science+Business Media Dordrecht 2013 Original Paper Text 2013 ftpubmed https://doi.org/10.1007/s10867-013-9309-9 2014-06-08T00:48:32Z Transmembrane acid–base fluxes affect the intracellular pH and unstirred layer pH around a superfused biological preparation. In this paper the factors influencing the unstirred layer pH and its gradient are studied. An analytical expression of the unstirred layer pH gradient in steady state is derived as a function of simultaneous transmembrane fluxes of (weak) acids and bases with the dehydration reaction of carbonic acid in equilibrium. Also a multicompartment computer model is described consisting of the extracellular bulk compartment, different unstirred layer compartments and the intracellular compartment. With this model also transient changes and the influence of carbonic anhydrase (CA) can be studied. The analytical expression and simulations with the multicompartment model demonstrate that in steady state the unstirred layer pH and its gradient are influenced by the size and type of transmembrane flux of acids and bases, their dissociation constant and diffusion coefficient, the concentration, diffusion coefficient and type of mobile buffers and the activity and location of CA. Similar principles contribute to the amplitude of the unstirred layer pH transients. According to these models an immobile buffer does not influence the steady-state pH, but reduces the amplitude of pH transients especially when these are fast. The unstirred layer pH provides useful information about transmembrane acid–base fluxes. This paper gives more insight how the unstirred layer pH and its transients can be interpreted. Methodological issues are discussed. Text Carbonic acid PubMed Central (PMC) Journal of Biological Physics 39 3 515 564 |
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Original Paper |
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Original Paper Marrannes, Roger Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH |
topic_facet |
Original Paper |
description |
Transmembrane acid–base fluxes affect the intracellular pH and unstirred layer pH around a superfused biological preparation. In this paper the factors influencing the unstirred layer pH and its gradient are studied. An analytical expression of the unstirred layer pH gradient in steady state is derived as a function of simultaneous transmembrane fluxes of (weak) acids and bases with the dehydration reaction of carbonic acid in equilibrium. Also a multicompartment computer model is described consisting of the extracellular bulk compartment, different unstirred layer compartments and the intracellular compartment. With this model also transient changes and the influence of carbonic anhydrase (CA) can be studied. The analytical expression and simulations with the multicompartment model demonstrate that in steady state the unstirred layer pH and its gradient are influenced by the size and type of transmembrane flux of acids and bases, their dissociation constant and diffusion coefficient, the concentration, diffusion coefficient and type of mobile buffers and the activity and location of CA. Similar principles contribute to the amplitude of the unstirred layer pH transients. According to these models an immobile buffer does not influence the steady-state pH, but reduces the amplitude of pH transients especially when these are fast. The unstirred layer pH provides useful information about transmembrane acid–base fluxes. This paper gives more insight how the unstirred layer pH and its transients can be interpreted. Methodological issues are discussed. |
format |
Text |
author |
Marrannes, Roger |
author_facet |
Marrannes, Roger |
author_sort |
Marrannes, Roger |
title |
Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH |
title_short |
Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH |
title_full |
Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH |
title_fullStr |
Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH |
title_full_unstemmed |
Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH |
title_sort |
computer model of unstirred layer and intracellular ph changes. determinants of unstirred layer ph |
publisher |
Springer Netherlands |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689360 http://www.ncbi.nlm.nih.gov/pubmed/23860924 https://doi.org/10.1007/s10867-013-9309-9 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC http://www.ncbi.nlm.nih.gov/pubmed/23860924 http://dx.doi.org/10.1007/s10867-013-9309-9 |
op_rights |
© Springer Science+Business Media Dordrecht 2013 |
op_doi |
https://doi.org/10.1007/s10867-013-9309-9 |
container_title |
Journal of Biological Physics |
container_volume |
39 |
container_issue |
3 |
container_start_page |
515 |
op_container_end_page |
564 |
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1766387879611727872 |