The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric e...
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ftpubmed:oai:pubmedcentral.nih.gov:3684784 2023-05-15T17:50:28+02:00 The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios Rouco, Mónica Branson, Oscar Lebrato, Mario Iglesias-Rodríguez, M. Débora 2013-06-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684784 http://www.ncbi.nlm.nih.gov/pubmed/23785363 https://doi.org/10.3389/fmicb.2013.00155 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684784 http://www.ncbi.nlm.nih.gov/pubmed/23785363 http://dx.doi.org/10.3389/fmicb.2013.00155 Copyright © 2013 Rouco, Branson, Lebrato and Iglesias-Rodríguez. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. CC-BY Microbiology Text 2013 ftpubmed https://doi.org/10.3389/fmicb.2013.00155 2013-09-05T01:13:06Z Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 μatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 μatm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification. Text Ocean acidification PubMed Central (PMC) Frontiers in Microbiology 4 |
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English |
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Microbiology |
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Microbiology Rouco, Mónica Branson, Oscar Lebrato, Mario Iglesias-Rodríguez, M. Débora The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios |
topic_facet |
Microbiology |
description |
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 μatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 μatm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification. |
format |
Text |
author |
Rouco, Mónica Branson, Oscar Lebrato, Mario Iglesias-Rodríguez, M. Débora |
author_facet |
Rouco, Mónica Branson, Oscar Lebrato, Mario Iglesias-Rodríguez, M. Débora |
author_sort |
Rouco, Mónica |
title |
The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios |
title_short |
The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios |
title_full |
The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios |
title_fullStr |
The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios |
title_full_unstemmed |
The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios |
title_sort |
effect of nitrate and phosphate availability on emiliania huxleyi (nzeh) physiology under different co2 scenarios |
publisher |
Frontiers Media S.A. |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684784 http://www.ncbi.nlm.nih.gov/pubmed/23785363 https://doi.org/10.3389/fmicb.2013.00155 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684784 http://www.ncbi.nlm.nih.gov/pubmed/23785363 http://dx.doi.org/10.3389/fmicb.2013.00155 |
op_rights |
Copyright © 2013 Rouco, Branson, Lebrato and Iglesias-Rodríguez. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2013.00155 |
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Frontiers in Microbiology |
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4 |
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1766157239568039936 |