Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse
Higher plants are an integral part of strategies for sustained human presence in space. Space-based greenhouses have the potential to provide closed-loop recycling of oxygen, water and food. Plant monitoring systems with the capacity to remotely observe the condition of crops in real-time within the...
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Molecular Diversity Preservation International (MDPI)
2013
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658760 http://www.ncbi.nlm.nih.gov/pubmed/23486220 https://doi.org/10.3390/s130303530 |
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ftpubmed:oai:pubmedcentral.nih.gov:3658760 2023-05-15T14:59:14+02:00 Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse Abboud, Talal Bamsey, Matthew Paul, Anna-Lisa Graham, Thomas Braham, Stephen Noumeir, Rita Berinstain, Alain Ferl, Robert 2013-03-13 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658760 http://www.ncbi.nlm.nih.gov/pubmed/23486220 https://doi.org/10.3390/s130303530 en eng Molecular Diversity Preservation International (MDPI) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658760 http://www.ncbi.nlm.nih.gov/pubmed/23486220 http://dx.doi.org/10.3390/s130303530 © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). CC-BY Article Text 2013 ftpubmed https://doi.org/10.3390/s130303530 2013-09-04T23:59:07Z Higher plants are an integral part of strategies for sustained human presence in space. Space-based greenhouses have the potential to provide closed-loop recycling of oxygen, water and food. Plant monitoring systems with the capacity to remotely observe the condition of crops in real-time within these systems would permit operators to take immediate action to ensure optimum system yield and reliability. One such plant health monitoring technique involves the use of reporter genes driving fluorescent proteins as biological sensors of plant stress. In 2006 an initial prototype green fluorescent protein imager system was deployed at the Arthur Clarke Mars Greenhouse located in the Canadian High Arctic. This prototype demonstrated the advantageous of this biosensor technology and underscored the challenges in collecting and managing telemetric data from exigent environments. We present here the design and deployment of a second prototype imaging system deployed within and connected to the infrastructure of the Arthur Clarke Mars Greenhouse. This is the first imager to run autonomously for one year in the un-crewed greenhouse with command and control conducted through the greenhouse satellite control system. Images were saved locally in high resolution and sent telemetrically in low resolution. Imager hardware is described, including the custom designed LED growth light and fluorescent excitation light boards, filters, data acquisition and control system, and basic sensing and environmental control. Several critical lessons learned related to the hardware of small plant growth payloads are also elaborated. Text Arctic PubMed Central (PMC) Arctic Sensors 13 3 3530 3548 |
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Article Abboud, Talal Bamsey, Matthew Paul, Anna-Lisa Graham, Thomas Braham, Stephen Noumeir, Rita Berinstain, Alain Ferl, Robert Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
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description |
Higher plants are an integral part of strategies for sustained human presence in space. Space-based greenhouses have the potential to provide closed-loop recycling of oxygen, water and food. Plant monitoring systems with the capacity to remotely observe the condition of crops in real-time within these systems would permit operators to take immediate action to ensure optimum system yield and reliability. One such plant health monitoring technique involves the use of reporter genes driving fluorescent proteins as biological sensors of plant stress. In 2006 an initial prototype green fluorescent protein imager system was deployed at the Arthur Clarke Mars Greenhouse located in the Canadian High Arctic. This prototype demonstrated the advantageous of this biosensor technology and underscored the challenges in collecting and managing telemetric data from exigent environments. We present here the design and deployment of a second prototype imaging system deployed within and connected to the infrastructure of the Arthur Clarke Mars Greenhouse. This is the first imager to run autonomously for one year in the un-crewed greenhouse with command and control conducted through the greenhouse satellite control system. Images were saved locally in high resolution and sent telemetrically in low resolution. Imager hardware is described, including the custom designed LED growth light and fluorescent excitation light boards, filters, data acquisition and control system, and basic sensing and environmental control. Several critical lessons learned related to the hardware of small plant growth payloads are also elaborated. |
format |
Text |
author |
Abboud, Talal Bamsey, Matthew Paul, Anna-Lisa Graham, Thomas Braham, Stephen Noumeir, Rita Berinstain, Alain Ferl, Robert |
author_facet |
Abboud, Talal Bamsey, Matthew Paul, Anna-Lisa Graham, Thomas Braham, Stephen Noumeir, Rita Berinstain, Alain Ferl, Robert |
author_sort |
Abboud, Talal |
title |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_short |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_full |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_fullStr |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_full_unstemmed |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_sort |
deployment of a fully-automated green fluorescent protein imaging system in a high arctic autonomous greenhouse |
publisher |
Molecular Diversity Preservation International (MDPI) |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658760 http://www.ncbi.nlm.nih.gov/pubmed/23486220 https://doi.org/10.3390/s130303530 |
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Arctic |
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Arctic |
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Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658760 http://www.ncbi.nlm.nih.gov/pubmed/23486220 http://dx.doi.org/10.3390/s130303530 |
op_rights |
© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/s130303530 |
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Sensors |
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13 |
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