Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter
The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low-energy conditions of the winter, but little is known about the specific phylotypes that have the p...
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ftpubmed:oai:pubmedcentral.nih.gov:3657686 2023-05-15T14:35:33+02:00 Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter Galand, Pierre E. Alonso-Sáez, Laura Bertilsson, Stefan Lovejoy, Connie Casamayor, Emilio O. 2013-05-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657686 http://www.ncbi.nlm.nih.gov/pubmed/23750155 https://doi.org/10.3389/fmicb.2013.00118 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657686 http://www.ncbi.nlm.nih.gov/pubmed/23750155 http://dx.doi.org/10.3389/fmicb.2013.00118 Copyright © Galand, Alonso-Sáez,Bertilsson, Lovejoy and Casamayor. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. CC-BY Microbiology Text 2013 ftpubmed https://doi.org/10.3389/fmicb.2013.00118 2013-09-04T23:55:53Z The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low-energy conditions of the winter, but little is known about the specific phylotypes that have the potential to survive and grow in such harsh environment. In this study, we aimed at identifying actively growing ribotypes in winter Arctic Ocean seawater cultures by experimental incubations with the thymidine analog bromodeoxyuridine (BrdU), followed by immunocapturing, terminal restriction fragment length polymorphism fingerprinting, cloning, and sequencing the 16S rRNA gene. We incubated water collected at different months over the Arctic winter and showed that the actively growing bacterial fraction, taking up BrdU, represented only a subset of the total community. Among the BrdU-labeled bacterial taxa we identified the Flavobacteria Polaribacter, the Alphaproteobacteria SAR11, the Gammaproteobacteria Arctic 96B-16 cluster and, predominately, members of Colwellia spp. Interestingly, Colwellia sequences formed three clusters (93 and 97% pairwise 16S rRNA identity) that contributed in contrasting ways to the active communities in the incubations. Polaribacter, Arctic 96B-16 and one cluster of Colwellia were more abundant in the active community represented by the BrdU-labeled DNA. In contrast, SAR11 and two other Colwellia clusters were underrepresented in the BrdU-labeled community compared to total communities. Despite the limitation of the long incubations needed to label slow growing arctic communities, the BrdU approach revealed the potential for active growth in low-energy conditions in some relevant groups of polar bacteria, including Polaribacter and Arctic 96B-16. Moreover, under similar incubation conditions, the growth of different Colwellia ribotypes varied, suggesting that related clusters of Colwellia may have distinct metabolic features. Text Arctic Arctic Ocean PubMed Central (PMC) Arctic Arctic Ocean Frontiers in Microbiology 4 |
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Microbiology |
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Microbiology Galand, Pierre E. Alonso-Sáez, Laura Bertilsson, Stefan Lovejoy, Connie Casamayor, Emilio O. Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
topic_facet |
Microbiology |
description |
The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low-energy conditions of the winter, but little is known about the specific phylotypes that have the potential to survive and grow in such harsh environment. In this study, we aimed at identifying actively growing ribotypes in winter Arctic Ocean seawater cultures by experimental incubations with the thymidine analog bromodeoxyuridine (BrdU), followed by immunocapturing, terminal restriction fragment length polymorphism fingerprinting, cloning, and sequencing the 16S rRNA gene. We incubated water collected at different months over the Arctic winter and showed that the actively growing bacterial fraction, taking up BrdU, represented only a subset of the total community. Among the BrdU-labeled bacterial taxa we identified the Flavobacteria Polaribacter, the Alphaproteobacteria SAR11, the Gammaproteobacteria Arctic 96B-16 cluster and, predominately, members of Colwellia spp. Interestingly, Colwellia sequences formed three clusters (93 and 97% pairwise 16S rRNA identity) that contributed in contrasting ways to the active communities in the incubations. Polaribacter, Arctic 96B-16 and one cluster of Colwellia were more abundant in the active community represented by the BrdU-labeled DNA. In contrast, SAR11 and two other Colwellia clusters were underrepresented in the BrdU-labeled community compared to total communities. Despite the limitation of the long incubations needed to label slow growing arctic communities, the BrdU approach revealed the potential for active growth in low-energy conditions in some relevant groups of polar bacteria, including Polaribacter and Arctic 96B-16. Moreover, under similar incubation conditions, the growth of different Colwellia ribotypes varied, suggesting that related clusters of Colwellia may have distinct metabolic features. |
format |
Text |
author |
Galand, Pierre E. Alonso-Sáez, Laura Bertilsson, Stefan Lovejoy, Connie Casamayor, Emilio O. |
author_facet |
Galand, Pierre E. Alonso-Sáez, Laura Bertilsson, Stefan Lovejoy, Connie Casamayor, Emilio O. |
author_sort |
Galand, Pierre E. |
title |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_short |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_full |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_fullStr |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_full_unstemmed |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_sort |
contrasting activity patterns determined by brdu incorporation in bacterial ribotypes from the arctic ocean in winter |
publisher |
Frontiers Media S.A. |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657686 http://www.ncbi.nlm.nih.gov/pubmed/23750155 https://doi.org/10.3389/fmicb.2013.00118 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657686 http://www.ncbi.nlm.nih.gov/pubmed/23750155 http://dx.doi.org/10.3389/fmicb.2013.00118 |
op_rights |
Copyright © Galand, Alonso-Sáez,Bertilsson, Lovejoy and Casamayor. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2013.00118 |
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Frontiers in Microbiology |
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4 |
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1766308361658171392 |