Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission
We investigated the influence of altered gravity on key proteins of T cell activation during the MASER-12 ballistic suborbital rocket mission of the European Space Agency (ESA) and the Swedish Space Cooperation (SSC) at ESRANGE Space Center (Kiruna, Sweden). We quantified components of the T cell re...
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ftpubmed:oai:pubmedcentral.nih.gov:3653714 2023-05-15T17:04:19+02:00 Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission Tauber, Svantje Hauschild, Swantje Crescio, Claudia Secchi, Christian Paulsen, Katrin Pantaleo, Antonella Saba, Angela Buttron, Isabell Thiel, Cora Sandra Cogoli, Augusto Pippia, Proto Ullrich, Oliver 2013-05-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653714 http://www.ncbi.nlm.nih.gov/pubmed/23651740 https://doi.org/10.1186/1478-811X-11-32 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653714 http://www.ncbi.nlm.nih.gov/pubmed/23651740 http://dx.doi.org/10.1186/1478-811X-11-32 Copyright ©2013 Tauber et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Text 2013 ftpubmed https://doi.org/10.1186/1478-811X-11-32 2013-09-04T23:43:56Z We investigated the influence of altered gravity on key proteins of T cell activation during the MASER-12 ballistic suborbital rocket mission of the European Space Agency (ESA) and the Swedish Space Cooperation (SSC) at ESRANGE Space Center (Kiruna, Sweden). We quantified components of the T cell receptor, the membrane proximal signaling, MAPK-signaling, IL-2R, histone modifications and the cytoskeleton in non-activated and in ConA/CD28-activated primary human T lymphocytes. The hypergravity phase during the launch resulted in a downregulation of the IL-2 and CD3 receptor and reduction of tyrosine phosphorylation, p44/42-MAPK phosphorylation and histone H3 acetylation, whereas LAT phosphorylation was increased. Compared to the baseline situation at the point of entry into the microgravity phase, CD3 and IL-2 receptor expression at the surface of non-activated T cells were reduced after 6 min microgravity. Importantly, p44/42-MAPK-phosphorylation was also reduced after 6 min microgravity compared to the 1g ground controls, but also in direct comparison between the in-flight μg and the 1g group. In activated T cells, the reduced CD3 and IL-2 receptor expression at the baseline situation recovered significantly during in-flight 1g conditions, but not during microgravity conditions. Beta-tubulin increased significantly after onset of microgravity until the end of the microgravity phase, but not in the in-flight 1g condition. This study suggests that key proteins of T cell signal modules are not severely disturbed in microgravity. Instead, it can be supposed that the strong T cell inhibiting signal occurs downstream from membrane proximal signaling, such as at the transcriptional level as described recently. However, the MASER-12 experiment could identify signal molecules, which are sensitive to altered gravity, and indicates that gravity is obviously not only a requirement for transcriptional processes as described before, but also for specific phosphorylation / dephosphorylation of signal molecules and surface ... Text Kiruna PubMed Central (PMC) Esrange ENVELOPE(21.117,21.117,67.883,67.883) Kiruna Cell Communication and Signaling 11 1 32 |
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Research Tauber, Svantje Hauschild, Swantje Crescio, Claudia Secchi, Christian Paulsen, Katrin Pantaleo, Antonella Saba, Angela Buttron, Isabell Thiel, Cora Sandra Cogoli, Augusto Pippia, Proto Ullrich, Oliver Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission |
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Research |
description |
We investigated the influence of altered gravity on key proteins of T cell activation during the MASER-12 ballistic suborbital rocket mission of the European Space Agency (ESA) and the Swedish Space Cooperation (SSC) at ESRANGE Space Center (Kiruna, Sweden). We quantified components of the T cell receptor, the membrane proximal signaling, MAPK-signaling, IL-2R, histone modifications and the cytoskeleton in non-activated and in ConA/CD28-activated primary human T lymphocytes. The hypergravity phase during the launch resulted in a downregulation of the IL-2 and CD3 receptor and reduction of tyrosine phosphorylation, p44/42-MAPK phosphorylation and histone H3 acetylation, whereas LAT phosphorylation was increased. Compared to the baseline situation at the point of entry into the microgravity phase, CD3 and IL-2 receptor expression at the surface of non-activated T cells were reduced after 6 min microgravity. Importantly, p44/42-MAPK-phosphorylation was also reduced after 6 min microgravity compared to the 1g ground controls, but also in direct comparison between the in-flight μg and the 1g group. In activated T cells, the reduced CD3 and IL-2 receptor expression at the baseline situation recovered significantly during in-flight 1g conditions, but not during microgravity conditions. Beta-tubulin increased significantly after onset of microgravity until the end of the microgravity phase, but not in the in-flight 1g condition. This study suggests that key proteins of T cell signal modules are not severely disturbed in microgravity. Instead, it can be supposed that the strong T cell inhibiting signal occurs downstream from membrane proximal signaling, such as at the transcriptional level as described recently. However, the MASER-12 experiment could identify signal molecules, which are sensitive to altered gravity, and indicates that gravity is obviously not only a requirement for transcriptional processes as described before, but also for specific phosphorylation / dephosphorylation of signal molecules and surface ... |
format |
Text |
author |
Tauber, Svantje Hauschild, Swantje Crescio, Claudia Secchi, Christian Paulsen, Katrin Pantaleo, Antonella Saba, Angela Buttron, Isabell Thiel, Cora Sandra Cogoli, Augusto Pippia, Proto Ullrich, Oliver |
author_facet |
Tauber, Svantje Hauschild, Swantje Crescio, Claudia Secchi, Christian Paulsen, Katrin Pantaleo, Antonella Saba, Angela Buttron, Isabell Thiel, Cora Sandra Cogoli, Augusto Pippia, Proto Ullrich, Oliver |
author_sort |
Tauber, Svantje |
title |
Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission |
title_short |
Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission |
title_full |
Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission |
title_fullStr |
Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission |
title_full_unstemmed |
Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission |
title_sort |
signal transduction in primary human t lymphocytes in altered gravity – results of the maser-12 suborbital space flight mission |
publisher |
BioMed Central |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653714 http://www.ncbi.nlm.nih.gov/pubmed/23651740 https://doi.org/10.1186/1478-811X-11-32 |
long_lat |
ENVELOPE(21.117,21.117,67.883,67.883) |
geographic |
Esrange Kiruna |
geographic_facet |
Esrange Kiruna |
genre |
Kiruna |
genre_facet |
Kiruna |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653714 http://www.ncbi.nlm.nih.gov/pubmed/23651740 http://dx.doi.org/10.1186/1478-811X-11-32 |
op_rights |
Copyright ©2013 Tauber et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/1478-811X-11-32 |
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Cell Communication and Signaling |
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11 |
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32 |
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1766058385215586304 |