In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts
The dinoflagellate Dinophysis spp. is responsible for diarrhetic shellfish poisoning (DSP). In the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (DTX3), the 7-OH acylated form of either okadaic acid (OA) or DTX1, is produced. We demonstrated in vitro acylation of OA wi...
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ftpubmed:oai:pubmedcentral.nih.gov:3640381 2023-05-15T15:58:44+02:00 In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts Konoki, Keiichi Onoda, Tatsuya Watanabe, Ryuichi Cho, Yuko Kaga, Shinnosuke Suzuki, Toshiyuki Yotsu-Yamashita, Mari 2013-01-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640381 http://www.ncbi.nlm.nih.gov/pubmed/23434830 https://doi.org/10.3390/md11020300 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640381 http://www.ncbi.nlm.nih.gov/pubmed/23434830 http://dx.doi.org/10.3390/md11020300 © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). CC-BY Article Text 2013 ftpubmed https://doi.org/10.3390/md11020300 2013-09-04T23:07:56Z The dinoflagellate Dinophysis spp. is responsible for diarrhetic shellfish poisoning (DSP). In the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (DTX3), the 7-OH acylated form of either okadaic acid (OA) or DTX1, is produced. We demonstrated in vitro acylation of OA with palmitoyl CoA in the presence of protein extract from the digestive gland, but not other tissues of the bivalve Mizuhopecten yessoensis. The yield of 7-O-palmitoyl OA reached its maximum within 2 h, was the highest at 37 °C followed by 28 °C, 16 °C and 4 °C and was the highest at pH 8 in comparison with the yields at pH 6 and pH 4. The transformation also proceeded when the protein extract was prepared from the bivalves Corbicula japonica and Crassostrea gigas. The OA binding protein OABP2 identified in the sponge Halichondria okadai was not detected in the bivalve M. yessoensis, the bivalve Mytilus galloprovincialis and the ascidian Halocynthia roretzi, though they are known to accumulate diarrhetic shellfish poisoning toxins. Since DTX3 does not bind to protein phosphatases 1 and 2A, the physiological target for OA and DTXs in mammalian cells, the acylation of DSP toxins would be related to a detoxification mechanism for the bivalve species. Text Crassostrea gigas PubMed Central (PMC) Marine Drugs 11 12 300 315 |
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Article Konoki, Keiichi Onoda, Tatsuya Watanabe, Ryuichi Cho, Yuko Kaga, Shinnosuke Suzuki, Toshiyuki Yotsu-Yamashita, Mari In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
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The dinoflagellate Dinophysis spp. is responsible for diarrhetic shellfish poisoning (DSP). In the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (DTX3), the 7-OH acylated form of either okadaic acid (OA) or DTX1, is produced. We demonstrated in vitro acylation of OA with palmitoyl CoA in the presence of protein extract from the digestive gland, but not other tissues of the bivalve Mizuhopecten yessoensis. The yield of 7-O-palmitoyl OA reached its maximum within 2 h, was the highest at 37 °C followed by 28 °C, 16 °C and 4 °C and was the highest at pH 8 in comparison with the yields at pH 6 and pH 4. The transformation also proceeded when the protein extract was prepared from the bivalves Corbicula japonica and Crassostrea gigas. The OA binding protein OABP2 identified in the sponge Halichondria okadai was not detected in the bivalve M. yessoensis, the bivalve Mytilus galloprovincialis and the ascidian Halocynthia roretzi, though they are known to accumulate diarrhetic shellfish poisoning toxins. Since DTX3 does not bind to protein phosphatases 1 and 2A, the physiological target for OA and DTXs in mammalian cells, the acylation of DSP toxins would be related to a detoxification mechanism for the bivalve species. |
format |
Text |
author |
Konoki, Keiichi Onoda, Tatsuya Watanabe, Ryuichi Cho, Yuko Kaga, Shinnosuke Suzuki, Toshiyuki Yotsu-Yamashita, Mari |
author_facet |
Konoki, Keiichi Onoda, Tatsuya Watanabe, Ryuichi Cho, Yuko Kaga, Shinnosuke Suzuki, Toshiyuki Yotsu-Yamashita, Mari |
author_sort |
Konoki, Keiichi |
title |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_short |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_full |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_fullStr |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_full_unstemmed |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_sort |
in vitro acylation of okadaic acid in the presence of various bivalves’ extracts |
publisher |
MDPI |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640381 http://www.ncbi.nlm.nih.gov/pubmed/23434830 https://doi.org/10.3390/md11020300 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3640381 http://www.ncbi.nlm.nih.gov/pubmed/23434830 http://dx.doi.org/10.3390/md11020300 |
op_rights |
© 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md11020300 |
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Marine Drugs |
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11 |
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12 |
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300 |
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315 |
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1766394508388335616 |