Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved β-Tubulin ResidueV⃞
The microtubule cytoskeleton is involved in regulation of cell morphology, differentiation, and cell cycle progression. Precisely controlled dynamic properties are required for these microtubule functions. To better understand how tubulin's dynamics are embedded in its primary sequence, we inve...
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ftpubmed:oai:pubmedcentral.nih.gov:363098 2023-05-15T13:55:02+02:00 Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved β-Tubulin ResidueV⃞ Paluh, Janet L. Killilea, Alison N. Detrich, H. William Downing, Kenneth H. 2004-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363098 http://www.ncbi.nlm.nih.gov/pubmed/14657251 https://doi.org/10.1091/mbc.E03-06-0389 en eng The American Society for Cell Biology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363098 http://www.ncbi.nlm.nih.gov/pubmed/14657251 http://dx.doi.org/10.1091/mbc.E03-06-0389 Copyright © 2004, The American Society for Cell Biology Articles Text 2004 ftpubmed https://doi.org/10.1091/mbc.E03-06-0389 2013-08-29T21:31:55Z The microtubule cytoskeleton is involved in regulation of cell morphology, differentiation, and cell cycle progression. Precisely controlled dynamic properties are required for these microtubule functions. To better understand how tubulin's dynamics are embedded in its primary sequence, we investigated in vivo the consequences of altering a single, highly conserved residue in β-tubulin that lies at the interface between two structural domains. The residue differs between the cold-adapted Antarctic fish and temperate animals in a manner that suggests a role in microtubule stability. Fungi, like the Antarctic fish, have a phenylalanine in this position, whereas essentially all other animals have tyrosine. We mutated the corresponding residue in fission yeast to tyrosine. Temperature effects were subtle, but time-lapse microscopy of microtubule dynamics revealed reduced depolymerization rates and increased stability. Mitotic exit signaled by breakdown of the mitotic spindle was delayed. In meiosis, microtubules displayed prolonged contact to the cell cortex during horsetail movement, followed by completion of meiosis I but frequent asymmetric failure of meiosis II spindle formation. Our results indicate that depolymerization dynamics modulated through interdomain motion may be important for regulating a subset of plus-end microtubule complexes in Schizosaccharomyces pombe. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Molecular Biology of the Cell 15 3 1160 1171 |
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Articles Paluh, Janet L. Killilea, Alison N. Detrich, H. William Downing, Kenneth H. Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved β-Tubulin ResidueV⃞ |
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The microtubule cytoskeleton is involved in regulation of cell morphology, differentiation, and cell cycle progression. Precisely controlled dynamic properties are required for these microtubule functions. To better understand how tubulin's dynamics are embedded in its primary sequence, we investigated in vivo the consequences of altering a single, highly conserved residue in β-tubulin that lies at the interface between two structural domains. The residue differs between the cold-adapted Antarctic fish and temperate animals in a manner that suggests a role in microtubule stability. Fungi, like the Antarctic fish, have a phenylalanine in this position, whereas essentially all other animals have tyrosine. We mutated the corresponding residue in fission yeast to tyrosine. Temperature effects were subtle, but time-lapse microscopy of microtubule dynamics revealed reduced depolymerization rates and increased stability. Mitotic exit signaled by breakdown of the mitotic spindle was delayed. In meiosis, microtubules displayed prolonged contact to the cell cortex during horsetail movement, followed by completion of meiosis I but frequent asymmetric failure of meiosis II spindle formation. Our results indicate that depolymerization dynamics modulated through interdomain motion may be important for regulating a subset of plus-end microtubule complexes in Schizosaccharomyces pombe. |
format |
Text |
author |
Paluh, Janet L. Killilea, Alison N. Detrich, H. William Downing, Kenneth H. |
author_facet |
Paluh, Janet L. Killilea, Alison N. Detrich, H. William Downing, Kenneth H. |
author_sort |
Paluh, Janet L. |
title |
Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved β-Tubulin ResidueV⃞ |
title_short |
Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved β-Tubulin ResidueV⃞ |
title_full |
Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved β-Tubulin ResidueV⃞ |
title_fullStr |
Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved β-Tubulin ResidueV⃞ |
title_full_unstemmed |
Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved β-Tubulin ResidueV⃞ |
title_sort |
meiosis-specific failure of cell cycle progression in fission yeast by mutation of a conserved β-tubulin residuev⃞ |
publisher |
The American Society for Cell Biology |
publishDate |
2004 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363098 http://www.ncbi.nlm.nih.gov/pubmed/14657251 https://doi.org/10.1091/mbc.E03-06-0389 |
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Antarctic The Antarctic |
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Antarctic The Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363098 http://www.ncbi.nlm.nih.gov/pubmed/14657251 http://dx.doi.org/10.1091/mbc.E03-06-0389 |
op_rights |
Copyright © 2004, The American Society for Cell Biology |
op_doi |
https://doi.org/10.1091/mbc.E03-06-0389 |
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Molecular Biology of the Cell |
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15 |
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3 |
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1160 |
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1171 |
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1766261266057265152 |