Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia
Rats are considered the principal maintenance hosts of Leptospira. The objectives of this study were isolation and identification of Leptospira serovars circulating among urban rat populations in Kuala Lumpur. Three hundred urban rats (73% Rattus rattus and 27% R. norvegicus) from three different si...
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ftpubmed:oai:pubmedcentral.nih.gov:3617856 2023-05-15T18:05:16+02:00 Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia Benacer, Douadi Zain, Siti Nursheena Mohd Amran, Fairuz Galloway, Renee L. Thong, Kwai Lin 2013-04-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617856 http://www.ncbi.nlm.nih.gov/pubmed/23358635 https://doi.org/10.4269/ajtmh.12-0662 en eng The American Society of Tropical Medicine and Hygiene http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617856 http://www.ncbi.nlm.nih.gov/pubmed/23358635 http://dx.doi.org/10.4269/ajtmh.12-0662 ©The American Society of Tropical Medicine and Hygiene Articles Text 2013 ftpubmed https://doi.org/10.4269/ajtmh.12-0662 2014-04-06T00:40:53Z Rats are considered the principal maintenance hosts of Leptospira. The objectives of this study were isolation and identification of Leptospira serovars circulating among urban rat populations in Kuala Lumpur. Three hundred urban rats (73% Rattus rattus and 27% R. norvegicus) from three different sites were trapped. Twenty cultures were positive for Leptospira using dark-field microscopy. R. rattus was the dominant carrier (70%). Polymerase chain reaction (PCR) confirmed that all isolates were pathogenic Leptospira species. Two Leptospira serogroups, Javanica and Bataviae, were identified using microscopic agglutination test (MAT). Pulsed-field gel electrophoresis (PFGE) identified two serovars in the urban rat populations: L. borgpetersenii serovar Javanica (85%) and L. interrogans serovar Bataviae (15%). We conclude that these two serovars are the major serovars circulating among the urban rat populations in Kuala Lumpur. Despite the low infection rate reported, the high pathogenicity of these serovars raises concern of public health risks caused by rodent transmission of leptospirosis. Text Rattus rattus PubMed Central (PMC) The American Journal of Tropical Medicine and Hygiene 88 4 704 709 |
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Articles Benacer, Douadi Zain, Siti Nursheena Mohd Amran, Fairuz Galloway, Renee L. Thong, Kwai Lin Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia |
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Rats are considered the principal maintenance hosts of Leptospira. The objectives of this study were isolation and identification of Leptospira serovars circulating among urban rat populations in Kuala Lumpur. Three hundred urban rats (73% Rattus rattus and 27% R. norvegicus) from three different sites were trapped. Twenty cultures were positive for Leptospira using dark-field microscopy. R. rattus was the dominant carrier (70%). Polymerase chain reaction (PCR) confirmed that all isolates were pathogenic Leptospira species. Two Leptospira serogroups, Javanica and Bataviae, were identified using microscopic agglutination test (MAT). Pulsed-field gel electrophoresis (PFGE) identified two serovars in the urban rat populations: L. borgpetersenii serovar Javanica (85%) and L. interrogans serovar Bataviae (15%). We conclude that these two serovars are the major serovars circulating among the urban rat populations in Kuala Lumpur. Despite the low infection rate reported, the high pathogenicity of these serovars raises concern of public health risks caused by rodent transmission of leptospirosis. |
format |
Text |
author |
Benacer, Douadi Zain, Siti Nursheena Mohd Amran, Fairuz Galloway, Renee L. Thong, Kwai Lin |
author_facet |
Benacer, Douadi Zain, Siti Nursheena Mohd Amran, Fairuz Galloway, Renee L. Thong, Kwai Lin |
author_sort |
Benacer, Douadi |
title |
Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia |
title_short |
Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia |
title_full |
Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia |
title_fullStr |
Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia |
title_full_unstemmed |
Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia |
title_sort |
isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of kuala lumpur, malaysia |
publisher |
The American Society of Tropical Medicine and Hygiene |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617856 http://www.ncbi.nlm.nih.gov/pubmed/23358635 https://doi.org/10.4269/ajtmh.12-0662 |
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Rattus rattus |
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Rattus rattus |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617856 http://www.ncbi.nlm.nih.gov/pubmed/23358635 http://dx.doi.org/10.4269/ajtmh.12-0662 |
op_rights |
©The American Society of Tropical Medicine and Hygiene |
op_doi |
https://doi.org/10.4269/ajtmh.12-0662 |
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The American Journal of Tropical Medicine and Hygiene |
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88 |
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4 |
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704 |
op_container_end_page |
709 |
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1766176740117315584 |