Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers
Little is known about the viability of eukaryotic microorganisms preserved in icy regions. Here we report on the diversity of microbial eukaryotes in ice samples derived from four Pyrenean glaciers. The species composition of eukaryotic communities in these glaciers is unknown mostly because of the...
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ftpubmed:oai:pubmedcentral.nih.gov:3601304 2023-05-15T16:38:21+02:00 Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers García-Descalzo, Laura García-López, Eva Postigo, Marina Baquero, Fernando Alcazar, Alberto Cid, Cristina 2013-03-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601304 http://www.ncbi.nlm.nih.gov/pubmed/23515855 https://doi.org/10.3389/fmicb.2013.00055 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601304 http://www.ncbi.nlm.nih.gov/pubmed/23515855 http://dx.doi.org/10.3389/fmicb.2013.00055 Copyright © García-Descalzo, García-López, Postigo, Baquero, Alcazar and Cid. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. CC-BY Microbiology Text 2013 ftpubmed https://doi.org/10.3389/fmicb.2013.00055 2013-09-04T21:19:48Z Little is known about the viability of eukaryotic microorganisms preserved in icy regions. Here we report on the diversity of microbial eukaryotes in ice samples derived from four Pyrenean glaciers. The species composition of eukaryotic communities in these glaciers is unknown mostly because of the presence of a multi-year ice cap, and it is not clear whether they harbor the same populations. The recent deglaciation of these areas is allowing an easy access to glacial layers that correspond to the “Little Ice Age” although some isolated deposits are attributed to previous glacial cycles. In this study, we use molecular 18S rRNA-based approaches to characterize some of the microbial eukaryotic populations associated with Pyrenean glaciers. Firstly, we performed a chemical and microscopical characterization of ice samples. Secondly, molecular analyses revealed interesting protist genetic diversity in glaciers. In order to understand the microbial composition of the ice samples the eukaryotic communities resident in the glacial samples were examined by amplifying community DNA and constructing clone libraries with 18S rRNA primers. After removal of potential chimeric sequences and dereplication of identical sequences, phylogenetic analysis demonstrated that several different protists could be identified. Protist diversity was more phylum rich in Aneto and Monte Perdido glaciers. The dominant taxonomic groups across all samples (>1% of all sequences) were Viridiplantae and Rhizaria. Significant variations in relative abundances of protist phyla between higher and lower glaciers were observed. At the genus level, significant differences were also recorded for the dominant genera Chloromonas, Raphidonema, Heteromita, Koliella, and Bodomorpha. In addition, protist community structure showed significant differences between glaciers. The relative abundances of protist groups at different taxonomic levels correlated with the altitude and area of glaciers and with pH of ice, but little or no relationships to other ... Text Ice cap PubMed Central (PMC) Frontiers in Microbiology 4 |
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Microbiology |
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Microbiology García-Descalzo, Laura García-López, Eva Postigo, Marina Baquero, Fernando Alcazar, Alberto Cid, Cristina Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers |
topic_facet |
Microbiology |
description |
Little is known about the viability of eukaryotic microorganisms preserved in icy regions. Here we report on the diversity of microbial eukaryotes in ice samples derived from four Pyrenean glaciers. The species composition of eukaryotic communities in these glaciers is unknown mostly because of the presence of a multi-year ice cap, and it is not clear whether they harbor the same populations. The recent deglaciation of these areas is allowing an easy access to glacial layers that correspond to the “Little Ice Age” although some isolated deposits are attributed to previous glacial cycles. In this study, we use molecular 18S rRNA-based approaches to characterize some of the microbial eukaryotic populations associated with Pyrenean glaciers. Firstly, we performed a chemical and microscopical characterization of ice samples. Secondly, molecular analyses revealed interesting protist genetic diversity in glaciers. In order to understand the microbial composition of the ice samples the eukaryotic communities resident in the glacial samples were examined by amplifying community DNA and constructing clone libraries with 18S rRNA primers. After removal of potential chimeric sequences and dereplication of identical sequences, phylogenetic analysis demonstrated that several different protists could be identified. Protist diversity was more phylum rich in Aneto and Monte Perdido glaciers. The dominant taxonomic groups across all samples (>1% of all sequences) were Viridiplantae and Rhizaria. Significant variations in relative abundances of protist phyla between higher and lower glaciers were observed. At the genus level, significant differences were also recorded for the dominant genera Chloromonas, Raphidonema, Heteromita, Koliella, and Bodomorpha. In addition, protist community structure showed significant differences between glaciers. The relative abundances of protist groups at different taxonomic levels correlated with the altitude and area of glaciers and with pH of ice, but little or no relationships to other ... |
format |
Text |
author |
García-Descalzo, Laura García-López, Eva Postigo, Marina Baquero, Fernando Alcazar, Alberto Cid, Cristina |
author_facet |
García-Descalzo, Laura García-López, Eva Postigo, Marina Baquero, Fernando Alcazar, Alberto Cid, Cristina |
author_sort |
García-Descalzo, Laura |
title |
Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers |
title_short |
Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers |
title_full |
Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers |
title_fullStr |
Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers |
title_full_unstemmed |
Eukaryotic microorganisms in cold environments: examples from Pyrenean glaciers |
title_sort |
eukaryotic microorganisms in cold environments: examples from pyrenean glaciers |
publisher |
Frontiers Media S.A. |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601304 http://www.ncbi.nlm.nih.gov/pubmed/23515855 https://doi.org/10.3389/fmicb.2013.00055 |
genre |
Ice cap |
genre_facet |
Ice cap |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601304 http://www.ncbi.nlm.nih.gov/pubmed/23515855 http://dx.doi.org/10.3389/fmicb.2013.00055 |
op_rights |
Copyright © García-Descalzo, García-López, Postigo, Baquero, Alcazar and Cid. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2013.00055 |
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Frontiers in Microbiology |
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4 |
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1766028618330275840 |