Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales)
Three cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined for Chlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing,...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591964 http://www.ncbi.nlm.nih.gov/pubmed/23275507 https://doi.org/10.1128/AEM.02899-12 |
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ftpubmed:oai:pubmedcentral.nih.gov:3591964 2023-05-15T14:30:11+02:00 Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) Stride, M. C. Polkinghorne, A. Miller, T. L. Groff, J. M. LaPatra, S. E. Nowak, B. F. 2013-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591964 http://www.ncbi.nlm.nih.gov/pubmed/23275507 https://doi.org/10.1128/AEM.02899-12 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591964 http://www.ncbi.nlm.nih.gov/pubmed/23275507 http://dx.doi.org/10.1128/AEM.02899-12 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Environmental Microbiology Text 2013 ftpubmed https://doi.org/10.1128/AEM.02899-12 2013-09-04T20:51:55Z Three cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined for Chlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the order Chlamydiales. A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported “Candidatus Piscichlamydia salmonis” (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35 Chlamydia and Chlamydia-like bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the order Chlamydiales. Based on these observations, we propose this bacterium of yellowtail kingfish be known as “Candidatus Parilichlamydia carangidicola” and that the new family be known as “Candidatus Parilichlamydiaceae.” Text Arctic charr Arctic Atlantic salmon PubMed Central (PMC) Arctic Applied and Environmental Microbiology 79 5 1590 1597 |
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English |
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Environmental Microbiology |
spellingShingle |
Environmental Microbiology Stride, M. C. Polkinghorne, A. Miller, T. L. Groff, J. M. LaPatra, S. E. Nowak, B. F. Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
topic_facet |
Environmental Microbiology |
description |
Three cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined for Chlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the order Chlamydiales. A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported “Candidatus Piscichlamydia salmonis” (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35 Chlamydia and Chlamydia-like bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the order Chlamydiales. Based on these observations, we propose this bacterium of yellowtail kingfish be known as “Candidatus Parilichlamydia carangidicola” and that the new family be known as “Candidatus Parilichlamydiaceae.” |
format |
Text |
author |
Stride, M. C. Polkinghorne, A. Miller, T. L. Groff, J. M. LaPatra, S. E. Nowak, B. F. |
author_facet |
Stride, M. C. Polkinghorne, A. Miller, T. L. Groff, J. M. LaPatra, S. E. Nowak, B. F. |
author_sort |
Stride, M. C. |
title |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_short |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_full |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_fullStr |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_full_unstemmed |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_sort |
molecular characterization of “candidatus parilichlamydia carangidicola,” a novel chlamydia-like epitheliocystis agent in yellowtail kingfish, seriola lalandi (valenciennes), and the proposal of a new family, “candidatus parilichlamydiaceae” fam. nov. (order chlamydiales) |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591964 http://www.ncbi.nlm.nih.gov/pubmed/23275507 https://doi.org/10.1128/AEM.02899-12 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Atlantic salmon |
genre_facet |
Arctic charr Arctic Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591964 http://www.ncbi.nlm.nih.gov/pubmed/23275507 http://dx.doi.org/10.1128/AEM.02899-12 |
op_rights |
Copyright © 2013, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.02899-12 |
container_title |
Applied and Environmental Microbiology |
container_volume |
79 |
container_issue |
5 |
container_start_page |
1590 |
op_container_end_page |
1597 |
_version_ |
1766304075687657472 |