Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant
Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma free Hb from red blood cell (rbc) re...
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ftpubmed:oai:pubmedcentral.nih.gov:3584015 2023-05-15T15:52:36+02:00 Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant Bian, Yuzhu Rong, Zhixia Chang, Thomas Ming Swi 2011-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584015 http://www.ncbi.nlm.nih.gov/pubmed/21574914 https://doi.org/10.3109/10731199.2011.581052 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584015 http://www.ncbi.nlm.nih.gov/pubmed/21574914 http://dx.doi.org/10.3109/10731199.2011.581052 Copyright © 2011 Informa Healthcare USA, Inc. Article Text 2011 ftpubmed https://doi.org/10.3109/10731199.2011.581052 2013-09-04T20:26:07Z Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma free Hb from red blood cell (rbc) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide. Text Carbonic acid PubMed Central (PMC) Artificial Cells, Blood Substitutes, and Biotechnology 39 3 127 136 |
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Article Bian, Yuzhu Rong, Zhixia Chang, Thomas Ming Swi Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant |
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Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma free Hb from red blood cell (rbc) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide. |
format |
Text |
author |
Bian, Yuzhu Rong, Zhixia Chang, Thomas Ming Swi |
author_facet |
Bian, Yuzhu Rong, Zhixia Chang, Thomas Ming Swi |
author_sort |
Bian, Yuzhu |
title |
Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant |
title_short |
Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant |
title_full |
Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant |
title_fullStr |
Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant |
title_full_unstemmed |
Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant |
title_sort |
polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584015 http://www.ncbi.nlm.nih.gov/pubmed/21574914 https://doi.org/10.3109/10731199.2011.581052 |
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Carbonic acid |
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Carbonic acid |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584015 http://www.ncbi.nlm.nih.gov/pubmed/21574914 http://dx.doi.org/10.3109/10731199.2011.581052 |
op_rights |
Copyright © 2011 Informa Healthcare USA, Inc. |
op_doi |
https://doi.org/10.3109/10731199.2011.581052 |
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Artificial Cells, Blood Substitutes, and Biotechnology |
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39 |
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3 |
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127 |
op_container_end_page |
136 |
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1766387730548260864 |