Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry
Although marine oysters contain abundant amounts of ether-linked aminophospholipids, the structural identification of the various molecular species has not been reported. We developed a normal-phase silica liquid chromatography/negative-ion electrospray ionization/quadrupole multiple-stage linear io...
Published in: | Analytica Chimica Acta |
---|---|
Main Authors: | , , |
Format: | Text |
Language: | English |
Published: |
2012
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566561 http://www.ncbi.nlm.nih.gov/pubmed/22713920 https://doi.org/10.1016/j.aca.2012.05.035 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:3566561 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:3566561 2023-05-15T17:54:20+02:00 Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry Chen, Su Belikova, Natalia A. Subbaiah, Papasani V. 2012-05-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566561 http://www.ncbi.nlm.nih.gov/pubmed/22713920 https://doi.org/10.1016/j.aca.2012.05.035 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566561 http://www.ncbi.nlm.nih.gov/pubmed/22713920 http://dx.doi.org/10.1016/j.aca.2012.05.035 © 2012 Elsevier B.V. All rights reserved. Article Text 2012 ftpubmed https://doi.org/10.1016/j.aca.2012.05.035 2013-09-04T19:33:33Z Although marine oysters contain abundant amounts of ether-linked aminophospholipids, the structural identification of the various molecular species has not been reported. We developed a normal-phase silica liquid chromatography/negative-ion electrospray ionization/quadrupole multiple-stage linear ion-trap mass spectrometric (NPLC-NI-ESI/Q-TRAP-MS3) method for the structural elucidation of ether molecular species of serine and ethanolamine phospholipids from marine oysters. The major advantages of the approach are (i) to avoid incorrect selection of isobaric precursor ions derived from different phospholipid classes in a lipid mixture, and to generate informative and clear MSn product ion mass spectra of the species for the identification of the sn-1 plasmanyl or plasmenyl linkages, and (ii) to increase precursor ion intensities by “concentrating” lipid molecules of each phospholipid class for further structural determination of minor molecular species. Employing a combination of NPLC-NI-ESI/MS3 and NPLC-NI-ESI/MS2, we elucidated, for the first time, the chemical structures of docosahexaenoyl and eicosapentaenoyl plasmenyl phosphatidylserine (PS) species and differentiated up to six isobaric species of diacyl/alkylacyl/alkenylacyl phosphatidylethanolamine (PE) in the US pacific oysters. The presence of a high content of both omega-3 plasmenyl PS/plasmenyl PE species and multiple isobaric molecular species isomers is the noteworthy characteristic of the marine oyster. The simple and robust NPLC-NI-ESI/MSn-based methodology should be particularly valuable in the detailed characterization of marine lipid dietary supplements with respect to omega-3 aminophospholipids. Text Pacific oyster PubMed Central (PMC) Pacific Analytica Chimica Acta 735 76 89 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Chen, Su Belikova, Natalia A. Subbaiah, Papasani V. Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry |
topic_facet |
Article |
description |
Although marine oysters contain abundant amounts of ether-linked aminophospholipids, the structural identification of the various molecular species has not been reported. We developed a normal-phase silica liquid chromatography/negative-ion electrospray ionization/quadrupole multiple-stage linear ion-trap mass spectrometric (NPLC-NI-ESI/Q-TRAP-MS3) method for the structural elucidation of ether molecular species of serine and ethanolamine phospholipids from marine oysters. The major advantages of the approach are (i) to avoid incorrect selection of isobaric precursor ions derived from different phospholipid classes in a lipid mixture, and to generate informative and clear MSn product ion mass spectra of the species for the identification of the sn-1 plasmanyl or plasmenyl linkages, and (ii) to increase precursor ion intensities by “concentrating” lipid molecules of each phospholipid class for further structural determination of minor molecular species. Employing a combination of NPLC-NI-ESI/MS3 and NPLC-NI-ESI/MS2, we elucidated, for the first time, the chemical structures of docosahexaenoyl and eicosapentaenoyl plasmenyl phosphatidylserine (PS) species and differentiated up to six isobaric species of diacyl/alkylacyl/alkenylacyl phosphatidylethanolamine (PE) in the US pacific oysters. The presence of a high content of both omega-3 plasmenyl PS/plasmenyl PE species and multiple isobaric molecular species isomers is the noteworthy characteristic of the marine oyster. The simple and robust NPLC-NI-ESI/MSn-based methodology should be particularly valuable in the detailed characterization of marine lipid dietary supplements with respect to omega-3 aminophospholipids. |
format |
Text |
author |
Chen, Su Belikova, Natalia A. Subbaiah, Papasani V. |
author_facet |
Chen, Su Belikova, Natalia A. Subbaiah, Papasani V. |
author_sort |
Chen, Su |
title |
Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry |
title_short |
Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry |
title_full |
Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry |
title_fullStr |
Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry |
title_full_unstemmed |
Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry |
title_sort |
structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566561 http://www.ncbi.nlm.nih.gov/pubmed/22713920 https://doi.org/10.1016/j.aca.2012.05.035 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566561 http://www.ncbi.nlm.nih.gov/pubmed/22713920 http://dx.doi.org/10.1016/j.aca.2012.05.035 |
op_rights |
© 2012 Elsevier B.V. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.aca.2012.05.035 |
container_title |
Analytica Chimica Acta |
container_volume |
735 |
container_start_page |
76 |
op_container_end_page |
89 |
_version_ |
1766162088544174080 |