Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells
Infectious salmon anaemia virus (ISAV), a member of the Orthomyxoviridae family, infects and causes disease in farmed Atlantic salmon (Salmo salar L.). Previous studies have shown Atlantic salmon endothelial cells to be the primary targets of ISAV infection. However, it is not known if cells other t...
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ftpubmed:oai:pubmedcentral.nih.gov:3560113 2023-05-15T15:28:07+02:00 Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells Weli, Simon Chioma Aamelfot, Maria Dale, Ole Bendik Koppang, Erling Olaf Falk, Knut 2013-01-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560113 http://www.ncbi.nlm.nih.gov/pubmed/23282149 https://doi.org/10.1186/1743-422X-10-5 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560113 http://www.ncbi.nlm.nih.gov/pubmed/23282149 http://dx.doi.org/10.1186/1743-422X-10-5 Copyright ©2013 Weli et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Text 2013 ftpubmed https://doi.org/10.1186/1743-422X-10-5 2013-09-04T19:13:12Z Infectious salmon anaemia virus (ISAV), a member of the Orthomyxoviridae family, infects and causes disease in farmed Atlantic salmon (Salmo salar L.). Previous studies have shown Atlantic salmon endothelial cells to be the primary targets of ISAV infection. However, it is not known if cells other than endothelial cells play a role in ISAV tropism. To further assess cell tropism, we examined ISAV infection of Atlantic salmon gill epithelial cells in vivo and in vitro. We demonstrated the susceptibility of epithelial cells to ISAV infection. On comparison of primary gill epithelial cell cultures with ISAV permissive fish cell cultures, we found the virus yield in primary gill epithelial cells to be comparable with that of salmon head kidney (SHK)-1 cells, but lower than TO or Atlantic salmon kidney (ASK)-II cells. Light and transmission electron microscopy (TEM) revealed that the primary gill cells possessed characteristics consistent with epithelial cells. Virus histochemistry showed that gill epithelial cells expressed 4-O-acetylated sialic acid which is recognized as the ISAV receptor. To the best of our knowledge, this is the first demonstration of ISAV infection in Atlantic salmon primary gill epithelial cells. This study thus broadens our understanding of cell tropism and transmission of ISAV in Atlantic salmon. Text Atlantic salmon Salmo salar PubMed Central (PMC) Virology Journal 10 1 |
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Research Weli, Simon Chioma Aamelfot, Maria Dale, Ole Bendik Koppang, Erling Olaf Falk, Knut Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
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Research |
description |
Infectious salmon anaemia virus (ISAV), a member of the Orthomyxoviridae family, infects and causes disease in farmed Atlantic salmon (Salmo salar L.). Previous studies have shown Atlantic salmon endothelial cells to be the primary targets of ISAV infection. However, it is not known if cells other than endothelial cells play a role in ISAV tropism. To further assess cell tropism, we examined ISAV infection of Atlantic salmon gill epithelial cells in vivo and in vitro. We demonstrated the susceptibility of epithelial cells to ISAV infection. On comparison of primary gill epithelial cell cultures with ISAV permissive fish cell cultures, we found the virus yield in primary gill epithelial cells to be comparable with that of salmon head kidney (SHK)-1 cells, but lower than TO or Atlantic salmon kidney (ASK)-II cells. Light and transmission electron microscopy (TEM) revealed that the primary gill cells possessed characteristics consistent with epithelial cells. Virus histochemistry showed that gill epithelial cells expressed 4-O-acetylated sialic acid which is recognized as the ISAV receptor. To the best of our knowledge, this is the first demonstration of ISAV infection in Atlantic salmon primary gill epithelial cells. This study thus broadens our understanding of cell tropism and transmission of ISAV in Atlantic salmon. |
format |
Text |
author |
Weli, Simon Chioma Aamelfot, Maria Dale, Ole Bendik Koppang, Erling Olaf Falk, Knut |
author_facet |
Weli, Simon Chioma Aamelfot, Maria Dale, Ole Bendik Koppang, Erling Olaf Falk, Knut |
author_sort |
Weli, Simon Chioma |
title |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_short |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_full |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_fullStr |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_full_unstemmed |
Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells |
title_sort |
infectious salmon anaemia virus infection of atlantic salmon gill epithelial cells |
publisher |
BioMed Central |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560113 http://www.ncbi.nlm.nih.gov/pubmed/23282149 https://doi.org/10.1186/1743-422X-10-5 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560113 http://www.ncbi.nlm.nih.gov/pubmed/23282149 http://dx.doi.org/10.1186/1743-422X-10-5 |
op_rights |
Copyright ©2013 Weli et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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CC-BY |
op_doi |
https://doi.org/10.1186/1743-422X-10-5 |
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Virology Journal |
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10 |
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1 |
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1766358498789031936 |