Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada
The distribution and levels of Clostridium botulinum type E were determined from field sites used by Inuit hunters for butchering seals along the coast of Nunavik. The incidence rates of C. botulinum type E in shoreline soil along the coast were 0, 50, and 87.5% among samples tested for the Hudson S...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
American Society for Microbiology
2013
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553760 http://www.ncbi.nlm.nih.gov/pubmed/23160120 https://doi.org/10.1128/AEM.05999-11 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:3553760 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:3553760 2023-05-15T16:35:23+02:00 Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada Leclair, Daniel Farber, Jeffrey M. Doidge, Bill Blanchfield, Burke Suppa, Sandy Pagotto, Franco Austin, John W. 2013-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553760 http://www.ncbi.nlm.nih.gov/pubmed/23160120 https://doi.org/10.1128/AEM.05999-11 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553760 http://www.ncbi.nlm.nih.gov/pubmed/23160120 http://dx.doi.org/10.1128/AEM.05999-11 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Environmental Microbiology Text 2013 ftpubmed https://doi.org/10.1128/AEM.05999-11 2013-09-04T18:51:52Z The distribution and levels of Clostridium botulinum type E were determined from field sites used by Inuit hunters for butchering seals along the coast of Nunavik. The incidence rates of C. botulinum type E in shoreline soil along the coast were 0, 50, and 87.5% among samples tested for the Hudson Strait, Hudson Bay, and Ungava Bay regions, respectively. Spores were detected in seawater or coastal rock surfaces from 17.6% of butchering sites, almost all of which were located in southern Ungava Bay. Concentrations of C. botulinum type E along the Ungava Bay coast were significantly higher than on the coasts of Hudson Strait and Hudson Bay, with the highest concentrations (270 to 1,800/kg of sample) found near butchering sites located along the mouths of large rivers. The Koksoak River contained high levels of C. botulinum type E, with the highest median concentration (270/kg) found in sediments of the marine portion of the river. C. botulinum type E was found in the intestinal contents (4.4%) and skins (1.4%) of seals. A high genetic biodiversity of C. botulinum type E isolates was observed among the 21 butchering sites and their surroundings along the Nunavik coastline, with 83% of isolates (44/53) yielding distinct pulsed-field gel electrophoresis genotypes. Multiple sources of C. botulinum type E may be involved in the contamination of seal meat during butchering in this region, but the risk of contamination appears to be much higher from environmental sources along the shoreline of southern Ungava Bay and the sediments of the Koksoak River. Text Hudson Bay Hudson Strait inuit Ungava Bay Nunavik PubMed Central (PMC) Canada Hudson Hudson Bay Hudson Strait ENVELOPE(-70.000,-70.000,62.000,62.000) Koksoak River ENVELOPE(-68.165,-68.165,58.534,58.534) Nunavik Ungava Bay ENVELOPE(-67.489,-67.489,59.498,59.498) Applied and Environmental Microbiology 79 2 646 654 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Environmental Microbiology |
spellingShingle |
Environmental Microbiology Leclair, Daniel Farber, Jeffrey M. Doidge, Bill Blanchfield, Burke Suppa, Sandy Pagotto, Franco Austin, John W. Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
topic_facet |
Environmental Microbiology |
description |
The distribution and levels of Clostridium botulinum type E were determined from field sites used by Inuit hunters for butchering seals along the coast of Nunavik. The incidence rates of C. botulinum type E in shoreline soil along the coast were 0, 50, and 87.5% among samples tested for the Hudson Strait, Hudson Bay, and Ungava Bay regions, respectively. Spores were detected in seawater or coastal rock surfaces from 17.6% of butchering sites, almost all of which were located in southern Ungava Bay. Concentrations of C. botulinum type E along the Ungava Bay coast were significantly higher than on the coasts of Hudson Strait and Hudson Bay, with the highest concentrations (270 to 1,800/kg of sample) found near butchering sites located along the mouths of large rivers. The Koksoak River contained high levels of C. botulinum type E, with the highest median concentration (270/kg) found in sediments of the marine portion of the river. C. botulinum type E was found in the intestinal contents (4.4%) and skins (1.4%) of seals. A high genetic biodiversity of C. botulinum type E isolates was observed among the 21 butchering sites and their surroundings along the Nunavik coastline, with 83% of isolates (44/53) yielding distinct pulsed-field gel electrophoresis genotypes. Multiple sources of C. botulinum type E may be involved in the contamination of seal meat during butchering in this region, but the risk of contamination appears to be much higher from environmental sources along the shoreline of southern Ungava Bay and the sediments of the Koksoak River. |
format |
Text |
author |
Leclair, Daniel Farber, Jeffrey M. Doidge, Bill Blanchfield, Burke Suppa, Sandy Pagotto, Franco Austin, John W. |
author_facet |
Leclair, Daniel Farber, Jeffrey M. Doidge, Bill Blanchfield, Burke Suppa, Sandy Pagotto, Franco Austin, John W. |
author_sort |
Leclair, Daniel |
title |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_short |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_full |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_fullStr |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_full_unstemmed |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_sort |
distribution of clostridium botulinum type e strains in nunavik, northern quebec, canada |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553760 http://www.ncbi.nlm.nih.gov/pubmed/23160120 https://doi.org/10.1128/AEM.05999-11 |
long_lat |
ENVELOPE(-70.000,-70.000,62.000,62.000) ENVELOPE(-68.165,-68.165,58.534,58.534) ENVELOPE(-67.489,-67.489,59.498,59.498) |
geographic |
Canada Hudson Hudson Bay Hudson Strait Koksoak River Nunavik Ungava Bay |
geographic_facet |
Canada Hudson Hudson Bay Hudson Strait Koksoak River Nunavik Ungava Bay |
genre |
Hudson Bay Hudson Strait inuit Ungava Bay Nunavik |
genre_facet |
Hudson Bay Hudson Strait inuit Ungava Bay Nunavik |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553760 http://www.ncbi.nlm.nih.gov/pubmed/23160120 http://dx.doi.org/10.1128/AEM.05999-11 |
op_rights |
Copyright © 2013, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.05999-11 |
container_title |
Applied and Environmental Microbiology |
container_volume |
79 |
container_issue |
2 |
container_start_page |
646 |
op_container_end_page |
654 |
_version_ |
1766025611447369728 |