The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function
Dietary inclusion of a bacterial meal has recently been shown to efficiently abolish soybean meal-induced enteritis in Atlantic salmon. The objective of this study was to investigate whether inclusion of this bacterial meal in the diet could abrogate disease development in a murine model of epitheli...
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2013
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536074 http://www.ncbi.nlm.nih.gov/pubmed/23064342 https://doi.org/10.1128/AEM.02464-12 |
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ftpubmed:oai:pubmedcentral.nih.gov:3536074 2023-05-15T15:32:46+02:00 The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function Kleiveland, Charlotte R. Hult, Lene T. Olsen Spetalen, Signe Kaldhusdal, Magne Christofferesen, Trine Eker Bengtsson, Oskar Romarheim, Odd Helge Jacobsen, Morten Lea, Tor 2013-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536074 http://www.ncbi.nlm.nih.gov/pubmed/23064342 https://doi.org/10.1128/AEM.02464-12 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536074 http://www.ncbi.nlm.nih.gov/pubmed/23064342 http://dx.doi.org/10.1128/AEM.02464-12 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Food Microbiology Text 2013 ftpubmed https://doi.org/10.1128/AEM.02464-12 2013-09-04T17:56:13Z Dietary inclusion of a bacterial meal has recently been shown to efficiently abolish soybean meal-induced enteritis in Atlantic salmon. The objective of this study was to investigate whether inclusion of this bacterial meal in the diet could abrogate disease development in a murine model of epithelial injury and colitis and thus possibly have therapeutic potential in human inflammatory bowel disease. C57BL/6N mice were fed ad libitum a control diet or an experimental diet containing 254 g/kg of body weight BioProtein, a bacterial meal consisting of Methylococcus capsulatus (Bath), together with the heterogenic bacteria Ralstonia sp., Brevibacillus agri, and Aneurinibacillus sp. At day 8, colitis was induced by 3.5% dextran sulfate sodium (DSS) ad libitum in the drinking water for 6 days. Symptoms of DSS treatment were less profound after prophylactic treatment with the diet containing the BioProtein. Colitis-associated parameters such as reduced body weight, colon shortening, and epithelial damage also showed significant improvement. Levels of acute-phase reactants, proteins whose plasma concentrations increase in response to inflammation, and neutrophil infiltration were reduced. On the other, increased epithelial cell proliferation and enhanced mucin 2 (Muc2) transcription indicated improved integrity of the colonic epithelial layer. BioProtein mainly consists of Methylococcus capsulatus (Bath) (88%). The results that we obtained when using a bacterial meal consisting of M. capsulatus (Bath) were similar to those obtained when using BioProtein in the DSS model. Our results show that a bacterial meal of the noncommensal bacterium M. capsulatus (Bath) has the potential to attenuate DSS-induced colitis in mice by enhancing colonic barrier function, as judged by increased epithelial proliferation and increased Muc2 transcription. Text Atlantic salmon PubMed Central (PMC) Applied and Environmental Microbiology 79 1 48 56 |
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English |
topic |
Food Microbiology |
spellingShingle |
Food Microbiology Kleiveland, Charlotte R. Hult, Lene T. Olsen Spetalen, Signe Kaldhusdal, Magne Christofferesen, Trine Eker Bengtsson, Oskar Romarheim, Odd Helge Jacobsen, Morten Lea, Tor The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function |
topic_facet |
Food Microbiology |
description |
Dietary inclusion of a bacterial meal has recently been shown to efficiently abolish soybean meal-induced enteritis in Atlantic salmon. The objective of this study was to investigate whether inclusion of this bacterial meal in the diet could abrogate disease development in a murine model of epithelial injury and colitis and thus possibly have therapeutic potential in human inflammatory bowel disease. C57BL/6N mice were fed ad libitum a control diet or an experimental diet containing 254 g/kg of body weight BioProtein, a bacterial meal consisting of Methylococcus capsulatus (Bath), together with the heterogenic bacteria Ralstonia sp., Brevibacillus agri, and Aneurinibacillus sp. At day 8, colitis was induced by 3.5% dextran sulfate sodium (DSS) ad libitum in the drinking water for 6 days. Symptoms of DSS treatment were less profound after prophylactic treatment with the diet containing the BioProtein. Colitis-associated parameters such as reduced body weight, colon shortening, and epithelial damage also showed significant improvement. Levels of acute-phase reactants, proteins whose plasma concentrations increase in response to inflammation, and neutrophil infiltration were reduced. On the other, increased epithelial cell proliferation and enhanced mucin 2 (Muc2) transcription indicated improved integrity of the colonic epithelial layer. BioProtein mainly consists of Methylococcus capsulatus (Bath) (88%). The results that we obtained when using a bacterial meal consisting of M. capsulatus (Bath) were similar to those obtained when using BioProtein in the DSS model. Our results show that a bacterial meal of the noncommensal bacterium M. capsulatus (Bath) has the potential to attenuate DSS-induced colitis in mice by enhancing colonic barrier function, as judged by increased epithelial proliferation and increased Muc2 transcription. |
format |
Text |
author |
Kleiveland, Charlotte R. Hult, Lene T. Olsen Spetalen, Signe Kaldhusdal, Magne Christofferesen, Trine Eker Bengtsson, Oskar Romarheim, Odd Helge Jacobsen, Morten Lea, Tor |
author_facet |
Kleiveland, Charlotte R. Hult, Lene T. Olsen Spetalen, Signe Kaldhusdal, Magne Christofferesen, Trine Eker Bengtsson, Oskar Romarheim, Odd Helge Jacobsen, Morten Lea, Tor |
author_sort |
Kleiveland, Charlotte R. |
title |
The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function |
title_short |
The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function |
title_full |
The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function |
title_fullStr |
The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function |
title_full_unstemmed |
The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function |
title_sort |
noncommensal bacterium methylococcus capsulatus (bath) ameliorates dextran sulfate (sodium salt)-induced ulcerative colitis by influencing mechanisms essential for maintenance of the colonic barrier function |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536074 http://www.ncbi.nlm.nih.gov/pubmed/23064342 https://doi.org/10.1128/AEM.02464-12 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536074 http://www.ncbi.nlm.nih.gov/pubmed/23064342 http://dx.doi.org/10.1128/AEM.02464-12 |
op_rights |
Copyright © 2013, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.02464-12 |
container_title |
Applied and Environmental Microbiology |
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79 |
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1 |
container_start_page |
48 |
op_container_end_page |
56 |
_version_ |
1766363250513936384 |