Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma
A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels (Anguilla anguilla) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The add...
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ftpubmed:oai:pubmedcentral.nih.gov:353329 2023-05-15T13:26:51+02:00 Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma Nagabayashi, Toshihiko Wolf, Ken 1979-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC353329 http://www.ncbi.nlm.nih.gov/pubmed/16789177 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC353329 http://www.ncbi.nlm.nih.gov/pubmed/16789177 Animal Viruses Text 1979 ftpubmed 2013-08-29T21:06:39Z A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels (Anguilla anguilla) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The addition of actinomycin D during the first 2.75 h of infection inhibits viral replication. As determined in sucrose gradients, the buoyant density of the virus is 1.19 g/cm3. EV-2 has a moderately pleomorphic spherical morphology; its diameter ranges from 80 to 140 nm. The virion has narrow, regularly spaced surface projections about 10 nm long. Replication in FHM cells at 15°C shows new infectivity appearing at 10 h postinfection and reaching a plateau at 20 h. Cytopathic effects consist of cell fusion, syncytia, and irregularly rounded cell masses. Viral antigen was detected in the cytoplasm of infected cells by specific immunofluorescence. Text Anguilla anguilla PubMed Central (PMC) |
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Animal Viruses |
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Animal Viruses Nagabayashi, Toshihiko Wolf, Ken Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma |
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Animal Viruses |
description |
A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels (Anguilla anguilla) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The addition of actinomycin D during the first 2.75 h of infection inhibits viral replication. As determined in sucrose gradients, the buoyant density of the virus is 1.19 g/cm3. EV-2 has a moderately pleomorphic spherical morphology; its diameter ranges from 80 to 140 nm. The virion has narrow, regularly spaced surface projections about 10 nm long. Replication in FHM cells at 15°C shows new infectivity appearing at 10 h postinfection and reaching a plateau at 20 h. Cytopathic effects consist of cell fusion, syncytia, and irregularly rounded cell masses. Viral antigen was detected in the cytoplasm of infected cells by specific immunofluorescence. |
format |
Text |
author |
Nagabayashi, Toshihiko Wolf, Ken |
author_facet |
Nagabayashi, Toshihiko Wolf, Ken |
author_sort |
Nagabayashi, Toshihiko |
title |
Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma |
title_short |
Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma |
title_full |
Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma |
title_fullStr |
Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma |
title_full_unstemmed |
Characterization of EV-2, a Virus Isolated from European Eels (Anguilla anguilla) with Stomatopapilloma |
title_sort |
characterization of ev-2, a virus isolated from european eels (anguilla anguilla) with stomatopapilloma |
publishDate |
1979 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC353329 http://www.ncbi.nlm.nih.gov/pubmed/16789177 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC353329 http://www.ncbi.nlm.nih.gov/pubmed/16789177 |
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1766394863397371904 |