Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria
Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty...
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ftpubmed:oai:pubmedcentral.nih.gov:3509530 2023-05-15T14:48:14+02:00 Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria Dong, Sheng Yang, Jie Zhang, Xi-Ying Shi, Mei Song, Xiao-Yan Chen, Xiu-Lan Zhang, Yu-Zhong 2012-11-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509530 http://www.ncbi.nlm.nih.gov/pubmed/23203272 https://doi.org/10.3390/md10112481 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509530 http://www.ncbi.nlm.nih.gov/pubmed/23203272 http://dx.doi.org/10.3390/md10112481 © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). CC-BY Article Text 2012 ftpubmed https://doi.org/10.3390/md10112481 2013-09-04T16:32:18Z Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty five strains belonging to nine genera were recovered from six Laminaria samples, in which Psychrobacter (33/65), Psychromonas (10/65) and Polaribacter (8/65) were the predominant groups. Moreover, 21 alginate lyase-excreting strains were further screened from these Laminaria-associated bacteria. These alginate lyase-excreting strains belong to five genera. Psychromonas (8/21), Psedoalteromonas (6/21) and Polaribacter (4/21) are the predominant genera, and Psychrobacter, Winogradskyella, Psychromonas and Polaribacter were first found to produce alginate lyases. The optimal temperatures for the growth and algiante lyase production of many strains were as low as 10–20 °C, indicating that they are psychrophilic bacteria. The alginate lyases produced by 11 strains showed the highest activity at 20–30 °C, indicating that these enzymes are cold-adapted enzymes. Some strians showed high levels of extracellular alginate lyase activity around 200 U/mL. These results suggest that these algiante lyase-excreting bacteria from the Arctic alga are good materials for studying bacterial cold-adapted alginate lyases. Text Arctic Arctic Ocean PubMed Central (PMC) Arctic Arctic Ocean Marine Drugs 10 12 2481 2491 |
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Article Dong, Sheng Yang, Jie Zhang, Xi-Ying Shi, Mei Song, Xiao-Yan Chen, Xiu-Lan Zhang, Yu-Zhong Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
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description |
Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty five strains belonging to nine genera were recovered from six Laminaria samples, in which Psychrobacter (33/65), Psychromonas (10/65) and Polaribacter (8/65) were the predominant groups. Moreover, 21 alginate lyase-excreting strains were further screened from these Laminaria-associated bacteria. These alginate lyase-excreting strains belong to five genera. Psychromonas (8/21), Psedoalteromonas (6/21) and Polaribacter (4/21) are the predominant genera, and Psychrobacter, Winogradskyella, Psychromonas and Polaribacter were first found to produce alginate lyases. The optimal temperatures for the growth and algiante lyase production of many strains were as low as 10–20 °C, indicating that they are psychrophilic bacteria. The alginate lyases produced by 11 strains showed the highest activity at 20–30 °C, indicating that these enzymes are cold-adapted enzymes. Some strians showed high levels of extracellular alginate lyase activity around 200 U/mL. These results suggest that these algiante lyase-excreting bacteria from the Arctic alga are good materials for studying bacterial cold-adapted alginate lyases. |
format |
Text |
author |
Dong, Sheng Yang, Jie Zhang, Xi-Ying Shi, Mei Song, Xiao-Yan Chen, Xiu-Lan Zhang, Yu-Zhong |
author_facet |
Dong, Sheng Yang, Jie Zhang, Xi-Ying Shi, Mei Song, Xiao-Yan Chen, Xiu-Lan Zhang, Yu-Zhong |
author_sort |
Dong, Sheng |
title |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_short |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_full |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_fullStr |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_full_unstemmed |
Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria |
title_sort |
cultivable alginate lyase-excreting bacteria associated with the arctic brown alga laminaria |
publisher |
MDPI |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509530 http://www.ncbi.nlm.nih.gov/pubmed/23203272 https://doi.org/10.3390/md10112481 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509530 http://www.ncbi.nlm.nih.gov/pubmed/23203272 http://dx.doi.org/10.3390/md10112481 |
op_rights |
© 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md10112481 |
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Marine Drugs |
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10 |
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12 |
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2491 |
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