Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff
By next generation transcriptome sequencing, it is possible to obtain data on both nucleotide sequence variation and gene expression. We have used this approach (RNA-Seq) to investigate the genetic basis for differences in plumage coloration and mating strategies in a non-model bird species, the ruf...
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ftpubmed:oai:pubmedcentral.nih.gov:3492775 2023-05-15T17:58:41+02:00 Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff Ekblom, Robert Farrell, Lindsay L Lank, David B Burke, Terry 2012-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492775 http://www.ncbi.nlm.nih.gov/pubmed/23145334 https://doi.org/10.1002/ece3.370 en eng Blackwell Publishing Ltd http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492775 http://www.ncbi.nlm.nih.gov/pubmed/23145334 http://dx.doi.org/10.1002/ece3.370 © 2012 Published by Blackwell Publishing Ltd. http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. CC-BY Original Research Text 2012 ftpubmed https://doi.org/10.1002/ece3.370 2013-09-04T15:38:46Z By next generation transcriptome sequencing, it is possible to obtain data on both nucleotide sequence variation and gene expression. We have used this approach (RNA-Seq) to investigate the genetic basis for differences in plumage coloration and mating strategies in a non-model bird species, the ruff (Philomachus pugnax). Ruff males show enormous variation in the coloration of ornamental feathers, used for individual recognition. This polymorphism is linked to reproductive strategies, with dark males (Independents) defending territories on leks against other Independents, whereas white morphs (Satellites) co-occupy Independent's courts without agonistic interactions. Previous work found a strong genetic component for mating strategy, but the genes involved were not identified. We present feather transcriptome data of more than 6,000 de-novo sequenced ruff genes (although with limited coverage for many of them). None of the identified genes showed significant expression divergence between males, but many genetic markers showed nucleotide differentiation between different color morphs and mating strategies. These include several feather keratin genes, splicing factors, and the Xg blood-group gene. Many of the genes with significant genetic structure between mating strategies have not yet been annotated and their functions remain to be elucidated. We also conducted in-depth investigations of 28 pre-identified coloration candidate genes. Two of these (EDNRB and TYR) were specifically expressed in black- and rust-colored males, respectively. We have demonstrated the utility of next generation transcriptome sequencing for identifying and genotyping large number of genetic markers in a non-model species without previous genomic resources, and highlight the potential of this approach for addressing the genetic basis of ecologically important variation. Text Philomachus pugnax Ruff PubMed Central (PMC) Ecology and Evolution 2 10 2485 2505 |
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Original Research Ekblom, Robert Farrell, Lindsay L Lank, David B Burke, Terry Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
topic_facet |
Original Research |
description |
By next generation transcriptome sequencing, it is possible to obtain data on both nucleotide sequence variation and gene expression. We have used this approach (RNA-Seq) to investigate the genetic basis for differences in plumage coloration and mating strategies in a non-model bird species, the ruff (Philomachus pugnax). Ruff males show enormous variation in the coloration of ornamental feathers, used for individual recognition. This polymorphism is linked to reproductive strategies, with dark males (Independents) defending territories on leks against other Independents, whereas white morphs (Satellites) co-occupy Independent's courts without agonistic interactions. Previous work found a strong genetic component for mating strategy, but the genes involved were not identified. We present feather transcriptome data of more than 6,000 de-novo sequenced ruff genes (although with limited coverage for many of them). None of the identified genes showed significant expression divergence between males, but many genetic markers showed nucleotide differentiation between different color morphs and mating strategies. These include several feather keratin genes, splicing factors, and the Xg blood-group gene. Many of the genes with significant genetic structure between mating strategies have not yet been annotated and their functions remain to be elucidated. We also conducted in-depth investigations of 28 pre-identified coloration candidate genes. Two of these (EDNRB and TYR) were specifically expressed in black- and rust-colored males, respectively. We have demonstrated the utility of next generation transcriptome sequencing for identifying and genotyping large number of genetic markers in a non-model species without previous genomic resources, and highlight the potential of this approach for addressing the genetic basis of ecologically important variation. |
format |
Text |
author |
Ekblom, Robert Farrell, Lindsay L Lank, David B Burke, Terry |
author_facet |
Ekblom, Robert Farrell, Lindsay L Lank, David B Burke, Terry |
author_sort |
Ekblom, Robert |
title |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_short |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_full |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_fullStr |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_full_unstemmed |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_sort |
gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
publisher |
Blackwell Publishing Ltd |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492775 http://www.ncbi.nlm.nih.gov/pubmed/23145334 https://doi.org/10.1002/ece3.370 |
genre |
Philomachus pugnax Ruff |
genre_facet |
Philomachus pugnax Ruff |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492775 http://www.ncbi.nlm.nih.gov/pubmed/23145334 http://dx.doi.org/10.1002/ece3.370 |
op_rights |
© 2012 Published by Blackwell Publishing Ltd. http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.370 |
container_title |
Ecology and Evolution |
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2 |
container_issue |
10 |
container_start_page |
2485 |
op_container_end_page |
2505 |
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1766167380174569472 |