Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages
Archaea assemblages from the Arctic Ocean and Antarctic waters were compared by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes amplified using the Archaea-specific primers 344f and 517r. Inspection of the DGGE fingerprints of 33 samples from the Arctic Ocean (from SCIC...
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ftpubmed:oai:pubmedcentral.nih.gov:348845 2023-05-15T13:43:50+02:00 Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages Bano, Nasreen Ruffin, Shomari Ransom, Briana Hollibaugh, James T. 2004-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC348845 http://www.ncbi.nlm.nih.gov/pubmed/14766555 https://doi.org/10.1128/AEM.70.2.781-789.2004 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC348845 http://www.ncbi.nlm.nih.gov/pubmed/14766555 http://dx.doi.org/10.1128/AEM.70.2.781-789.2004 Copyright © 2004, American Society for Microbiology Microbial Ecology Text 2004 ftpubmed https://doi.org/10.1128/AEM.70.2.781-789.2004 2013-08-29T20:53:52Z Archaea assemblages from the Arctic Ocean and Antarctic waters were compared by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes amplified using the Archaea-specific primers 344f and 517r. Inspection of the DGGE fingerprints of 33 samples from the Arctic Ocean (from SCICEX submarine cruises in 1995, 1996, and 1997) and 7 Antarctic samples from Gerlache Strait and Dallman Bay revealed that the richness of Archaea assemblages was greater in samples from deep water than in those from the upper water column in both polar oceans. DGGE banding patterns suggested that most of the Archaea ribotypes were common to both the Arctic Ocean and the Antarctic Ocean. However, some of the Euryarchaeota ribotypes were unique to each system. Cluster analysis of DGGE fingerprints revealed no seasonal variation but supported depth-related differences in the composition of the Arctic Ocean Archaea assemblage. The phylogenetic composition of the Archaea assemblage was determined by cloning and then sequencing amplicons obtained from the Archaea-specific primers 21f and 958r. Sequences of 198 clones from nine samples covering three seasons and all depths grouped with marine group I Crenarchaeota (111 clones), marine group II Euryarchaeota (86 clones), and group IV Euryarchaeota (1 clone). A sequence obtained only from a DGGE band was similar to those of the marine group III Euryarchaeota. Text Antarc* Antarctic Antarctic Ocean Arctic Arctic Ocean SCICEX PubMed Central (PMC) Antarctic Antarctic Ocean Arctic Arctic Ocean Gerlache ENVELOPE(99.033,99.033,-66.500,-66.500) Gerlache Strait ENVELOPE(-62.333,-62.333,-64.500,-64.500) The Antarctic Applied and Environmental Microbiology 70 2 781 789 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Bano, Nasreen Ruffin, Shomari Ransom, Briana Hollibaugh, James T. Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages |
topic_facet |
Microbial Ecology |
description |
Archaea assemblages from the Arctic Ocean and Antarctic waters were compared by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes amplified using the Archaea-specific primers 344f and 517r. Inspection of the DGGE fingerprints of 33 samples from the Arctic Ocean (from SCICEX submarine cruises in 1995, 1996, and 1997) and 7 Antarctic samples from Gerlache Strait and Dallman Bay revealed that the richness of Archaea assemblages was greater in samples from deep water than in those from the upper water column in both polar oceans. DGGE banding patterns suggested that most of the Archaea ribotypes were common to both the Arctic Ocean and the Antarctic Ocean. However, some of the Euryarchaeota ribotypes were unique to each system. Cluster analysis of DGGE fingerprints revealed no seasonal variation but supported depth-related differences in the composition of the Arctic Ocean Archaea assemblage. The phylogenetic composition of the Archaea assemblage was determined by cloning and then sequencing amplicons obtained from the Archaea-specific primers 21f and 958r. Sequences of 198 clones from nine samples covering three seasons and all depths grouped with marine group I Crenarchaeota (111 clones), marine group II Euryarchaeota (86 clones), and group IV Euryarchaeota (1 clone). A sequence obtained only from a DGGE band was similar to those of the marine group III Euryarchaeota. |
format |
Text |
author |
Bano, Nasreen Ruffin, Shomari Ransom, Briana Hollibaugh, James T. |
author_facet |
Bano, Nasreen Ruffin, Shomari Ransom, Briana Hollibaugh, James T. |
author_sort |
Bano, Nasreen |
title |
Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages |
title_short |
Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages |
title_full |
Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages |
title_fullStr |
Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages |
title_full_unstemmed |
Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages |
title_sort |
phylogenetic composition of arctic ocean archaeal assemblages and comparison with antarctic assemblages |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC348845 http://www.ncbi.nlm.nih.gov/pubmed/14766555 https://doi.org/10.1128/AEM.70.2.781-789.2004 |
long_lat |
ENVELOPE(99.033,99.033,-66.500,-66.500) ENVELOPE(-62.333,-62.333,-64.500,-64.500) |
geographic |
Antarctic Antarctic Ocean Arctic Arctic Ocean Gerlache Gerlache Strait The Antarctic |
geographic_facet |
Antarctic Antarctic Ocean Arctic Arctic Ocean Gerlache Gerlache Strait The Antarctic |
genre |
Antarc* Antarctic Antarctic Ocean Arctic Arctic Ocean SCICEX |
genre_facet |
Antarc* Antarctic Antarctic Ocean Arctic Arctic Ocean SCICEX |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC348845 http://www.ncbi.nlm.nih.gov/pubmed/14766555 http://dx.doi.org/10.1128/AEM.70.2.781-789.2004 |
op_rights |
Copyright © 2004, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.70.2.781-789.2004 |
container_title |
Applied and Environmental Microbiology |
container_volume |
70 |
container_issue |
2 |
container_start_page |
781 |
op_container_end_page |
789 |
_version_ |
1766193848960155648 |