Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.
The host-parasite relationship in human visceral leishmaniasis remains poorly understood. In the present study, pathophysiological and immunological changes were examined in BALB/c mice infected with Leishmania donovani. These animals developed chronic infection with massive hepatosplenomegaly and h...
Main Author: | |
---|---|
Format: | Text |
Language: | English |
Published: |
1982
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879 http://www.ncbi.nlm.nih.gov/pubmed/7152667 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:347879 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:347879 2023-05-15T18:26:51+02:00 Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. Reiner, N E 1982-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879 http://www.ncbi.nlm.nih.gov/pubmed/7152667 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879 http://www.ncbi.nlm.nih.gov/pubmed/7152667 Research Article Text 1982 ftpubmed 2013-08-29T20:51:03Z The host-parasite relationship in human visceral leishmaniasis remains poorly understood. In the present study, pathophysiological and immunological changes were examined in BALB/c mice infected with Leishmania donovani. These animals developed chronic infection with massive hepatosplenomegaly and hypergammaglobulinemia. In contrast to mice inoculated with 0.8 X 10(6) or 4 X 10(6) amastigotes, mice infected with 20 X 10(6) amastigotes failed to reduce liver parasite loads during 2 to 8 weeks of observation. At 8 weeks, liver size was increased by 26, 63, and 94%, respectively, in groups infected with 0.8 X 10(6), 4 X 10(6), or 20 X 10(6) amastigotes. Serum immunoglobulin G and M levels at 8 weeks in animals with the heaviest infection were increased by 53 and 80%, respectively, compared with controls. Specific antileishmanial antibodies were detected in the absence of antigen-specific delayed-type hypersensitivity or in vitro lymphocyte responses. Infection did not suppress the in vivo responses of mice to the non-parasite-related antigens sperm whale myoglobin or pneumococcal polysaccharide. Splenic mononuclear cell responses to phytohemagglutinin were suppressed as early as 2 weeks, and by 8 weeks, mice infected with 0.8 X 10(6), 4 X 10(6), or 20 X 10(6) amastigotes had phytohemagglutinin responses which were, respectively, 27.7, 13.9, and 15.8% of controls. Decreased phytohemagglutinin responses could not be related to reductions in splenic T cells; however, splenic B cells and macrophages were increased at 8 weeks of infection. The course of L. donovani infection and disease in BALB/c mice resembles events occurring in humans and should prove useful in defining mechanisms of immune alterations in visceral leishmaniasis. Text Sperm whale PubMed Central (PMC) |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Reiner, N E Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. |
topic_facet |
Research Article |
description |
The host-parasite relationship in human visceral leishmaniasis remains poorly understood. In the present study, pathophysiological and immunological changes were examined in BALB/c mice infected with Leishmania donovani. These animals developed chronic infection with massive hepatosplenomegaly and hypergammaglobulinemia. In contrast to mice inoculated with 0.8 X 10(6) or 4 X 10(6) amastigotes, mice infected with 20 X 10(6) amastigotes failed to reduce liver parasite loads during 2 to 8 weeks of observation. At 8 weeks, liver size was increased by 26, 63, and 94%, respectively, in groups infected with 0.8 X 10(6), 4 X 10(6), or 20 X 10(6) amastigotes. Serum immunoglobulin G and M levels at 8 weeks in animals with the heaviest infection were increased by 53 and 80%, respectively, compared with controls. Specific antileishmanial antibodies were detected in the absence of antigen-specific delayed-type hypersensitivity or in vitro lymphocyte responses. Infection did not suppress the in vivo responses of mice to the non-parasite-related antigens sperm whale myoglobin or pneumococcal polysaccharide. Splenic mononuclear cell responses to phytohemagglutinin were suppressed as early as 2 weeks, and by 8 weeks, mice infected with 0.8 X 10(6), 4 X 10(6), or 20 X 10(6) amastigotes had phytohemagglutinin responses which were, respectively, 27.7, 13.9, and 15.8% of controls. Decreased phytohemagglutinin responses could not be related to reductions in splenic T cells; however, splenic B cells and macrophages were increased at 8 weeks of infection. The course of L. donovani infection and disease in BALB/c mice resembles events occurring in humans and should prove useful in defining mechanisms of immune alterations in visceral leishmaniasis. |
format |
Text |
author |
Reiner, N E |
author_facet |
Reiner, N E |
author_sort |
Reiner, N E |
title |
Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. |
title_short |
Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. |
title_full |
Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. |
title_fullStr |
Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. |
title_full_unstemmed |
Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. |
title_sort |
host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. |
publishDate |
1982 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879 http://www.ncbi.nlm.nih.gov/pubmed/7152667 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879 http://www.ncbi.nlm.nih.gov/pubmed/7152667 |
_version_ |
1766208818362974208 |