Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.

The host-parasite relationship in human visceral leishmaniasis remains poorly understood. In the present study, pathophysiological and immunological changes were examined in BALB/c mice infected with Leishmania donovani. These animals developed chronic infection with massive hepatosplenomegaly and h...

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Main Author: Reiner, N E
Format: Text
Language:English
Published: 1982
Subjects:
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879
http://www.ncbi.nlm.nih.gov/pubmed/7152667
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spelling ftpubmed:oai:pubmedcentral.nih.gov:347879 2023-05-15T18:26:51+02:00 Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes. Reiner, N E 1982-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879 http://www.ncbi.nlm.nih.gov/pubmed/7152667 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879 http://www.ncbi.nlm.nih.gov/pubmed/7152667 Research Article Text 1982 ftpubmed 2013-08-29T20:51:03Z The host-parasite relationship in human visceral leishmaniasis remains poorly understood. In the present study, pathophysiological and immunological changes were examined in BALB/c mice infected with Leishmania donovani. These animals developed chronic infection with massive hepatosplenomegaly and hypergammaglobulinemia. In contrast to mice inoculated with 0.8 X 10(6) or 4 X 10(6) amastigotes, mice infected with 20 X 10(6) amastigotes failed to reduce liver parasite loads during 2 to 8 weeks of observation. At 8 weeks, liver size was increased by 26, 63, and 94%, respectively, in groups infected with 0.8 X 10(6), 4 X 10(6), or 20 X 10(6) amastigotes. Serum immunoglobulin G and M levels at 8 weeks in animals with the heaviest infection were increased by 53 and 80%, respectively, compared with controls. Specific antileishmanial antibodies were detected in the absence of antigen-specific delayed-type hypersensitivity or in vitro lymphocyte responses. Infection did not suppress the in vivo responses of mice to the non-parasite-related antigens sperm whale myoglobin or pneumococcal polysaccharide. Splenic mononuclear cell responses to phytohemagglutinin were suppressed as early as 2 weeks, and by 8 weeks, mice infected with 0.8 X 10(6), 4 X 10(6), or 20 X 10(6) amastigotes had phytohemagglutinin responses which were, respectively, 27.7, 13.9, and 15.8% of controls. Decreased phytohemagglutinin responses could not be related to reductions in splenic T cells; however, splenic B cells and macrophages were increased at 8 weeks of infection. The course of L. donovani infection and disease in BALB/c mice resembles events occurring in humans and should prove useful in defining mechanisms of immune alterations in visceral leishmaniasis. Text Sperm whale PubMed Central (PMC)
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Research Article
spellingShingle Research Article
Reiner, N E
Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.
topic_facet Research Article
description The host-parasite relationship in human visceral leishmaniasis remains poorly understood. In the present study, pathophysiological and immunological changes were examined in BALB/c mice infected with Leishmania donovani. These animals developed chronic infection with massive hepatosplenomegaly and hypergammaglobulinemia. In contrast to mice inoculated with 0.8 X 10(6) or 4 X 10(6) amastigotes, mice infected with 20 X 10(6) amastigotes failed to reduce liver parasite loads during 2 to 8 weeks of observation. At 8 weeks, liver size was increased by 26, 63, and 94%, respectively, in groups infected with 0.8 X 10(6), 4 X 10(6), or 20 X 10(6) amastigotes. Serum immunoglobulin G and M levels at 8 weeks in animals with the heaviest infection were increased by 53 and 80%, respectively, compared with controls. Specific antileishmanial antibodies were detected in the absence of antigen-specific delayed-type hypersensitivity or in vitro lymphocyte responses. Infection did not suppress the in vivo responses of mice to the non-parasite-related antigens sperm whale myoglobin or pneumococcal polysaccharide. Splenic mononuclear cell responses to phytohemagglutinin were suppressed as early as 2 weeks, and by 8 weeks, mice infected with 0.8 X 10(6), 4 X 10(6), or 20 X 10(6) amastigotes had phytohemagglutinin responses which were, respectively, 27.7, 13.9, and 15.8% of controls. Decreased phytohemagglutinin responses could not be related to reductions in splenic T cells; however, splenic B cells and macrophages were increased at 8 weeks of infection. The course of L. donovani infection and disease in BALB/c mice resembles events occurring in humans and should prove useful in defining mechanisms of immune alterations in visceral leishmaniasis.
format Text
author Reiner, N E
author_facet Reiner, N E
author_sort Reiner, N E
title Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.
title_short Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.
title_full Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.
title_fullStr Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.
title_full_unstemmed Host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.
title_sort host-parasite relationship in murine leishmaniasis: pathophysiological and immunological changes.
publishDate 1982
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879
http://www.ncbi.nlm.nih.gov/pubmed/7152667
genre Sperm whale
genre_facet Sperm whale
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347879
http://www.ncbi.nlm.nih.gov/pubmed/7152667
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