Small Changes in pH Have Direct Effects on Marine Bacterial Community Composition: A Microcosm Approach
As the atmospheric CO2 concentration rises, more CO2 will dissolve in the oceans, leading to a reduction in pH. Effects of ocean acidification on bacterial communities have mainly been studied in biologically complex systems, in which indirect effects, mediated through food web interactions, come in...
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ftpubmed:oai:pubmedcentral.nih.gov:3469576 2023-05-15T17:51:38+02:00 Small Changes in pH Have Direct Effects on Marine Bacterial Community Composition: A Microcosm Approach Krause, Evamaria Wichels, Antje Giménez, Luis Lunau, Mirko Schilhabel, Markus B. Gerdts, Gunnar 2012-10-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469576 http://www.ncbi.nlm.nih.gov/pubmed/23071704 https://doi.org/10.1371/journal.pone.0047035 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469576 http://www.ncbi.nlm.nih.gov/pubmed/23071704 http://dx.doi.org/10.1371/journal.pone.0047035 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2012 ftpubmed https://doi.org/10.1371/journal.pone.0047035 2013-09-04T14:23:44Z As the atmospheric CO2 concentration rises, more CO2 will dissolve in the oceans, leading to a reduction in pH. Effects of ocean acidification on bacterial communities have mainly been studied in biologically complex systems, in which indirect effects, mediated through food web interactions, come into play. These approaches come close to nature but suffer from low replication and neglect seasonality. To comprehensively investigate direct pH effects, we conducted highly-replicated laboratory acidification experiments with the natural bacterial community from Helgoland Roads (North Sea). Seasonal variability was accounted for by repeating the experiment four times (spring, summer, autumn, winter). Three dilution approaches were used to select for different ecological strategies, i.e. fast-growing or low-nutrient adapted bacteria. The pH levels investigated were in situ seawater pH (8.15–8.22), pH 7.82 and pH 7.67, representing the present-day situation and two acidification scenarios projected for the North Sea for the year 2100. In all seasons, both automated ribosomal intergenic spacer analysis and 16S ribosomal amplicon pyrosequencing revealed pH-dependent community shifts for two of the dilution approaches. Bacteria susceptible to changes in pH were different members of Gammaproteobacteria, Flavobacteriaceae, Rhodobacteraceae, Campylobacteraceae and further less abundant groups. Their specific response to reduced pH was often context-dependent. Bacterial abundance was not influenced by pH. Our findings suggest that already moderate changes in pH have the potential to cause compositional shifts, depending on the community assembly and environmental factors. By identifying pH-susceptible groups, this study provides insights for more directed, in-depth community analyses in large-scale and long-term experiments. Text Ocean acidification PubMed Central (PMC) Helgoland PLoS ONE 7 10 e47035 |
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Research Article Krause, Evamaria Wichels, Antje Giménez, Luis Lunau, Mirko Schilhabel, Markus B. Gerdts, Gunnar Small Changes in pH Have Direct Effects on Marine Bacterial Community Composition: A Microcosm Approach |
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Research Article |
description |
As the atmospheric CO2 concentration rises, more CO2 will dissolve in the oceans, leading to a reduction in pH. Effects of ocean acidification on bacterial communities have mainly been studied in biologically complex systems, in which indirect effects, mediated through food web interactions, come into play. These approaches come close to nature but suffer from low replication and neglect seasonality. To comprehensively investigate direct pH effects, we conducted highly-replicated laboratory acidification experiments with the natural bacterial community from Helgoland Roads (North Sea). Seasonal variability was accounted for by repeating the experiment four times (spring, summer, autumn, winter). Three dilution approaches were used to select for different ecological strategies, i.e. fast-growing or low-nutrient adapted bacteria. The pH levels investigated were in situ seawater pH (8.15–8.22), pH 7.82 and pH 7.67, representing the present-day situation and two acidification scenarios projected for the North Sea for the year 2100. In all seasons, both automated ribosomal intergenic spacer analysis and 16S ribosomal amplicon pyrosequencing revealed pH-dependent community shifts for two of the dilution approaches. Bacteria susceptible to changes in pH were different members of Gammaproteobacteria, Flavobacteriaceae, Rhodobacteraceae, Campylobacteraceae and further less abundant groups. Their specific response to reduced pH was often context-dependent. Bacterial abundance was not influenced by pH. Our findings suggest that already moderate changes in pH have the potential to cause compositional shifts, depending on the community assembly and environmental factors. By identifying pH-susceptible groups, this study provides insights for more directed, in-depth community analyses in large-scale and long-term experiments. |
format |
Text |
author |
Krause, Evamaria Wichels, Antje Giménez, Luis Lunau, Mirko Schilhabel, Markus B. Gerdts, Gunnar |
author_facet |
Krause, Evamaria Wichels, Antje Giménez, Luis Lunau, Mirko Schilhabel, Markus B. Gerdts, Gunnar |
author_sort |
Krause, Evamaria |
title |
Small Changes in pH Have Direct Effects on Marine Bacterial Community Composition: A Microcosm Approach |
title_short |
Small Changes in pH Have Direct Effects on Marine Bacterial Community Composition: A Microcosm Approach |
title_full |
Small Changes in pH Have Direct Effects on Marine Bacterial Community Composition: A Microcosm Approach |
title_fullStr |
Small Changes in pH Have Direct Effects on Marine Bacterial Community Composition: A Microcosm Approach |
title_full_unstemmed |
Small Changes in pH Have Direct Effects on Marine Bacterial Community Composition: A Microcosm Approach |
title_sort |
small changes in ph have direct effects on marine bacterial community composition: a microcosm approach |
publisher |
Public Library of Science |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469576 http://www.ncbi.nlm.nih.gov/pubmed/23071704 https://doi.org/10.1371/journal.pone.0047035 |
geographic |
Helgoland |
geographic_facet |
Helgoland |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469576 http://www.ncbi.nlm.nih.gov/pubmed/23071704 http://dx.doi.org/10.1371/journal.pone.0047035 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0047035 |
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PLoS ONE |
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7 |
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10 |
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e47035 |
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