Expression of the Infectious Salmon Anemia Virus Receptor on Atlantic Salmon Endothelial Cells Correlates with the Cell Tropism of the Virus
Infectious salmon anemia (ISA) is a World Organization for Animal Health (OIE)-listed disease of farmed Atlantic salmon, characterized by slowly developing anemia and circulatory disturbances. The disease is caused by ISA virus (ISAV) in the Orthomyxoviridae family; hence, it is related to influenza...
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American Society for Microbiology
2012
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457268 http://www.ncbi.nlm.nih.gov/pubmed/22811536 https://doi.org/10.1128/JVI.00047-12 |
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ftpubmed:oai:pubmedcentral.nih.gov:3457268 2023-05-15T15:30:17+02:00 Expression of the Infectious Salmon Anemia Virus Receptor on Atlantic Salmon Endothelial Cells Correlates with the Cell Tropism of the Virus Aamelfot, Maria Dale, Ole Bendik Weli, Simon Chioma Koppang, Erling Olaf Falk, Knut 2012-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457268 http://www.ncbi.nlm.nih.gov/pubmed/22811536 https://doi.org/10.1128/JVI.00047-12 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457268 http://www.ncbi.nlm.nih.gov/pubmed/22811536 http://dx.doi.org/10.1128/JVI.00047-12 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Virus-Cell Interactions Text 2012 ftpubmed https://doi.org/10.1128/JVI.00047-12 2013-09-04T13:43:24Z Infectious salmon anemia (ISA) is a World Organization for Animal Health (OIE)-listed disease of farmed Atlantic salmon, characterized by slowly developing anemia and circulatory disturbances. The disease is caused by ISA virus (ISAV) in the Orthomyxoviridae family; hence, it is related to influenza. Here we explore the pathogenesis of ISA by focusing on virus tropism, receptor tissue distribution, and pathological changes in experimentally and naturally infected Atlantic salmon. Using immunohistochemistry on ISAV-infected Atlantic salmon tissues with antibody to viral nucleoprotein, endotheliotropism was demonstrated. Endothelial cells lining the circulatory system were found to be infected, seemingly noncytolytic, and without vasculitis. No virus could be found in necrotic parenchymal cells. From endothelium, the virus budded apically and adsorbed to red blood cells (RBCs). No infection or replication within RBCs was detected, but hemophagocytosis was observed, possibly contributing to the severe anemia in fish with this disease. Similarly to what has been done in studies of influenza, we examined the pattern of virus attachment by using ISAV as a probe. Here we detected the preferred receptor of ISAV, 4-O-acetylated sialic acid (Neu4,5Ac2). To our knowledge, this is the first report demonstrating the in situ distribution of this sialic acid derivate. The pattern of virus attachment mirrored closely the distribution of infection, showing that the virus receptor is important for cell tropism, as well as for adsorption to RBCs. Text Atlantic salmon PubMed Central (PMC) Journal of Virology 86 19 10571 10578 |
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Virus-Cell Interactions |
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Virus-Cell Interactions Aamelfot, Maria Dale, Ole Bendik Weli, Simon Chioma Koppang, Erling Olaf Falk, Knut Expression of the Infectious Salmon Anemia Virus Receptor on Atlantic Salmon Endothelial Cells Correlates with the Cell Tropism of the Virus |
topic_facet |
Virus-Cell Interactions |
description |
Infectious salmon anemia (ISA) is a World Organization for Animal Health (OIE)-listed disease of farmed Atlantic salmon, characterized by slowly developing anemia and circulatory disturbances. The disease is caused by ISA virus (ISAV) in the Orthomyxoviridae family; hence, it is related to influenza. Here we explore the pathogenesis of ISA by focusing on virus tropism, receptor tissue distribution, and pathological changes in experimentally and naturally infected Atlantic salmon. Using immunohistochemistry on ISAV-infected Atlantic salmon tissues with antibody to viral nucleoprotein, endotheliotropism was demonstrated. Endothelial cells lining the circulatory system were found to be infected, seemingly noncytolytic, and without vasculitis. No virus could be found in necrotic parenchymal cells. From endothelium, the virus budded apically and adsorbed to red blood cells (RBCs). No infection or replication within RBCs was detected, but hemophagocytosis was observed, possibly contributing to the severe anemia in fish with this disease. Similarly to what has been done in studies of influenza, we examined the pattern of virus attachment by using ISAV as a probe. Here we detected the preferred receptor of ISAV, 4-O-acetylated sialic acid (Neu4,5Ac2). To our knowledge, this is the first report demonstrating the in situ distribution of this sialic acid derivate. The pattern of virus attachment mirrored closely the distribution of infection, showing that the virus receptor is important for cell tropism, as well as for adsorption to RBCs. |
format |
Text |
author |
Aamelfot, Maria Dale, Ole Bendik Weli, Simon Chioma Koppang, Erling Olaf Falk, Knut |
author_facet |
Aamelfot, Maria Dale, Ole Bendik Weli, Simon Chioma Koppang, Erling Olaf Falk, Knut |
author_sort |
Aamelfot, Maria |
title |
Expression of the Infectious Salmon Anemia Virus Receptor on Atlantic Salmon Endothelial Cells Correlates with the Cell Tropism of the Virus |
title_short |
Expression of the Infectious Salmon Anemia Virus Receptor on Atlantic Salmon Endothelial Cells Correlates with the Cell Tropism of the Virus |
title_full |
Expression of the Infectious Salmon Anemia Virus Receptor on Atlantic Salmon Endothelial Cells Correlates with the Cell Tropism of the Virus |
title_fullStr |
Expression of the Infectious Salmon Anemia Virus Receptor on Atlantic Salmon Endothelial Cells Correlates with the Cell Tropism of the Virus |
title_full_unstemmed |
Expression of the Infectious Salmon Anemia Virus Receptor on Atlantic Salmon Endothelial Cells Correlates with the Cell Tropism of the Virus |
title_sort |
expression of the infectious salmon anemia virus receptor on atlantic salmon endothelial cells correlates with the cell tropism of the virus |
publisher |
American Society for Microbiology |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457268 http://www.ncbi.nlm.nih.gov/pubmed/22811536 https://doi.org/10.1128/JVI.00047-12 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457268 http://www.ncbi.nlm.nih.gov/pubmed/22811536 http://dx.doi.org/10.1128/JVI.00047-12 |
op_rights |
Copyright © 2012, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/JVI.00047-12 |
container_title |
Journal of Virology |
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86 |
container_issue |
19 |
container_start_page |
10571 |
op_container_end_page |
10578 |
_version_ |
1766360733724966912 |