Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions
The bivalve Arctica islandica is extremely long lived (>400 years) and can tolerate long periods of hypoxia and anoxia. European populations differ in maximum life spans (MLSP) from 40 years in the Baltic to >400 years around Iceland. Characteristic behavior of A. islandica involves phases of...
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ftpubmed:oai:pubmedcentral.nih.gov:3446923 2023-05-15T15:22:31+02:00 Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions Philipp, Eva E. R. Wessels, Wiebke Gruber, Heike Strahl, Julia Wagner, Anika E. Ernst, Insa M. A. Rimbach, Gerald Kraemer, Lars Schreiber, Stefan Abele, Doris Rosenstiel, Philip 2012-09-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446923 http://www.ncbi.nlm.nih.gov/pubmed/23028566 https://doi.org/10.1371/journal.pone.0044621 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446923 http://www.ncbi.nlm.nih.gov/pubmed/23028566 http://dx.doi.org/10.1371/journal.pone.0044621 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2012 ftpubmed https://doi.org/10.1371/journal.pone.0044621 2013-09-04T13:09:59Z The bivalve Arctica islandica is extremely long lived (>400 years) and can tolerate long periods of hypoxia and anoxia. European populations differ in maximum life spans (MLSP) from 40 years in the Baltic to >400 years around Iceland. Characteristic behavior of A. islandica involves phases of metabolic rate depression (MRD) during which the animals burry into the sediment for several days. During these phases the shell water oxygen concentrations reaches hypoxic to anoxic levels, which possibly support the long life span of some populations. We investigated gene regulation in A. islandica from a long-lived (MLSP 150 years) German Bight population and the short-lived Baltic Sea population, experimentally exposed to different oxygen levels. A new A. islandica transcriptome enabled the identification of genes important during hypoxia/anoxia events and, more generally, gene mining for putative stress response and (anti-) aging genes. Expression changes of a) antioxidant defense: Catalase, Glutathione peroxidase, manganese and copper-zinc Superoxide dismutase; b) oxygen sensing and general stress response: Hypoxia inducible factor alpha, Prolyl hydroxylase and Heat-shock protein 70; and c) anaerobic capacity: Malate dehydrogenase and Octopine dehydrogenase, related transcripts were investigated. Exposed to low oxygen, German Bight individuals suppressed transcription of all investigated genes, whereas Baltic Sea bivalves enhanced gene transcription under anoxic incubation (0 kPa) and, further, decreased these transcription levels again during 6 h of re-oxygenation. Hypoxic and anoxic exposure and subsequent re-oxygenation in Baltic Sea animals did not lead to increased protein oxidation or induction of apoptosis, emphasizing considerable hypoxia/re-oxygenation tolerance in this species. The data suggest that the energy saving effect of MRD may not be an attribute of Baltic Sea A. islandica chronically exposed to high environmental variability (oxygenation, temperature, salinity). Contrary, higher physiological ... Text Arctica islandica Iceland PubMed Central (PMC) PLoS ONE 7 9 e44621 |
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Research Article Philipp, Eva E. R. Wessels, Wiebke Gruber, Heike Strahl, Julia Wagner, Anika E. Ernst, Insa M. A. Rimbach, Gerald Kraemer, Lars Schreiber, Stefan Abele, Doris Rosenstiel, Philip Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions |
topic_facet |
Research Article |
description |
The bivalve Arctica islandica is extremely long lived (>400 years) and can tolerate long periods of hypoxia and anoxia. European populations differ in maximum life spans (MLSP) from 40 years in the Baltic to >400 years around Iceland. Characteristic behavior of A. islandica involves phases of metabolic rate depression (MRD) during which the animals burry into the sediment for several days. During these phases the shell water oxygen concentrations reaches hypoxic to anoxic levels, which possibly support the long life span of some populations. We investigated gene regulation in A. islandica from a long-lived (MLSP 150 years) German Bight population and the short-lived Baltic Sea population, experimentally exposed to different oxygen levels. A new A. islandica transcriptome enabled the identification of genes important during hypoxia/anoxia events and, more generally, gene mining for putative stress response and (anti-) aging genes. Expression changes of a) antioxidant defense: Catalase, Glutathione peroxidase, manganese and copper-zinc Superoxide dismutase; b) oxygen sensing and general stress response: Hypoxia inducible factor alpha, Prolyl hydroxylase and Heat-shock protein 70; and c) anaerobic capacity: Malate dehydrogenase and Octopine dehydrogenase, related transcripts were investigated. Exposed to low oxygen, German Bight individuals suppressed transcription of all investigated genes, whereas Baltic Sea bivalves enhanced gene transcription under anoxic incubation (0 kPa) and, further, decreased these transcription levels again during 6 h of re-oxygenation. Hypoxic and anoxic exposure and subsequent re-oxygenation in Baltic Sea animals did not lead to increased protein oxidation or induction of apoptosis, emphasizing considerable hypoxia/re-oxygenation tolerance in this species. The data suggest that the energy saving effect of MRD may not be an attribute of Baltic Sea A. islandica chronically exposed to high environmental variability (oxygenation, temperature, salinity). Contrary, higher physiological ... |
format |
Text |
author |
Philipp, Eva E. R. Wessels, Wiebke Gruber, Heike Strahl, Julia Wagner, Anika E. Ernst, Insa M. A. Rimbach, Gerald Kraemer, Lars Schreiber, Stefan Abele, Doris Rosenstiel, Philip |
author_facet |
Philipp, Eva E. R. Wessels, Wiebke Gruber, Heike Strahl, Julia Wagner, Anika E. Ernst, Insa M. A. Rimbach, Gerald Kraemer, Lars Schreiber, Stefan Abele, Doris Rosenstiel, Philip |
author_sort |
Philipp, Eva E. R. |
title |
Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions |
title_short |
Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions |
title_full |
Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions |
title_fullStr |
Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions |
title_full_unstemmed |
Gene Expression and Physiological Changes of Different Populations of the Long-Lived Bivalve Arctica islandica under Low Oxygen Conditions |
title_sort |
gene expression and physiological changes of different populations of the long-lived bivalve arctica islandica under low oxygen conditions |
publisher |
Public Library of Science |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446923 http://www.ncbi.nlm.nih.gov/pubmed/23028566 https://doi.org/10.1371/journal.pone.0044621 |
genre |
Arctica islandica Iceland |
genre_facet |
Arctica islandica Iceland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446923 http://www.ncbi.nlm.nih.gov/pubmed/23028566 http://dx.doi.org/10.1371/journal.pone.0044621 |
op_rights |
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0044621 |
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PLoS ONE |
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7 |
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9 |
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e44621 |
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1766353170077843456 |