Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales
Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison (Bison bison) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from these samples...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426704 http://www.ncbi.nlm.nih.gov/pubmed/22773624 https://doi.org/10.1128/AEM.01036-12 |
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ftpubmed:oai:pubmedcentral.nih.gov:3426704 2023-05-15T18:44:18+02:00 Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales Stratilo, Chad W. Bader, Douglas E. 2012-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426704 http://www.ncbi.nlm.nih.gov/pubmed/22773624 https://doi.org/10.1128/AEM.01036-12 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426704 http://www.ncbi.nlm.nih.gov/pubmed/22773624 http://dx.doi.org/10.1128/AEM.01036-12 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Microbial Ecology Text 2012 ftpubmed https://doi.org/10.1128/AEM.01036-12 2013-09-04T11:58:59Z Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison (Bison bison) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from these samples and confirmed using phenotypic characterization and real-time PCR. Confirmed B. anthracis isolates were typed using multiple-locus variable-number tandem repeat analysis (MLVA15) and single-nucleotide-repeat analysis (SNRA). B. anthracis isolates split into two clades based on MLVA15, while SNRA allowed some isolates between carcass sites to be distinguished from each other. SNRA polymorphisms were also present within a single carcass site. Some isolates from different carcass sites having the same SNRA type had divergent MLVA types; this finding leads to questions about hierarchical typing methods and the robustness of the fine-scale typing of Bacillus anthracis. Text Wood Buffalo Wood Buffalo National Park Bison bison bison PubMed Central (PMC) Wood Buffalo ENVELOPE(-112.007,-112.007,57.664,57.664) Applied and Environmental Microbiology 78 18 6433 6437 |
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PubMed Central (PMC) |
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language |
English |
topic |
Microbial Ecology |
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Microbial Ecology Stratilo, Chad W. Bader, Douglas E. Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
topic_facet |
Microbial Ecology |
description |
Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison (Bison bison) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from these samples and confirmed using phenotypic characterization and real-time PCR. Confirmed B. anthracis isolates were typed using multiple-locus variable-number tandem repeat analysis (MLVA15) and single-nucleotide-repeat analysis (SNRA). B. anthracis isolates split into two clades based on MLVA15, while SNRA allowed some isolates between carcass sites to be distinguished from each other. SNRA polymorphisms were also present within a single carcass site. Some isolates from different carcass sites having the same SNRA type had divergent MLVA types; this finding leads to questions about hierarchical typing methods and the robustness of the fine-scale typing of Bacillus anthracis. |
format |
Text |
author |
Stratilo, Chad W. Bader, Douglas E. |
author_facet |
Stratilo, Chad W. Bader, Douglas E. |
author_sort |
Stratilo, Chad W. |
title |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_short |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_full |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_fullStr |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_full_unstemmed |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_sort |
genetic diversity among bacillus anthracis soil isolates at fine geographic scales |
publisher |
American Society for Microbiology |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426704 http://www.ncbi.nlm.nih.gov/pubmed/22773624 https://doi.org/10.1128/AEM.01036-12 |
long_lat |
ENVELOPE(-112.007,-112.007,57.664,57.664) |
geographic |
Wood Buffalo |
geographic_facet |
Wood Buffalo |
genre |
Wood Buffalo Wood Buffalo National Park Bison bison bison |
genre_facet |
Wood Buffalo Wood Buffalo National Park Bison bison bison |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426704 http://www.ncbi.nlm.nih.gov/pubmed/22773624 http://dx.doi.org/10.1128/AEM.01036-12 |
op_rights |
Copyright © 2012, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.01036-12 |
container_title |
Applied and Environmental Microbiology |
container_volume |
78 |
container_issue |
18 |
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6433 |
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6437 |
_version_ |
1766234947496968192 |