New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel†
The enzymatic preparation of biodiesel has been hampered by the lack of suitable solvents with desirable properties such as high lipase compatibility, low cost, low viscosity, high biodegradability, and ease of product separation. Recent interest in using ionic liquids (ILs) as advanced reaction med...
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ftpubmed:oai:pubmedcentral.nih.gov:3416871 2023-05-15T13:50:09+02:00 New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel† Zhao, Hua Baker, Gary A. Holmes, Shaletha 2011-01-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416871 http://www.ncbi.nlm.nih.gov/pubmed/21283901 https://doi.org/10.1039/c0ob01011a en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416871 http://www.ncbi.nlm.nih.gov/pubmed/21283901 http://dx.doi.org/10.1039/c0ob01011a © The Royal Society of Chemistry 2011 Article Text 2011 ftpubmed https://doi.org/10.1039/c0ob01011a 2013-09-04T11:21:55Z The enzymatic preparation of biodiesel has been hampered by the lack of suitable solvents with desirable properties such as high lipase compatibility, low cost, low viscosity, high biodegradability, and ease of product separation. Recent interest in using ionic liquids (ILs) as advanced reaction media has led to fast reaction rates and high yields in the enzymatic synthesis of biodiesel. However, conventional (i.e., cation–anion paired) ILs based on imidazolium and other quaternary ammonium salts remain too expensive for wide application at industrial scales. In this study, we report on newly-synthesized eutectic ILs derived from choline acetate or choline chloride coupled with biocompatible hydrogen-bond donors, such as glycerol. These eutectic solvents have favorable properties including low viscosity, high biodegradability, and excellent compatibility with Novozym® 435, a commercial immobilized Candida antarctica lipase B. Furthermore, in a model biodiesel synthesis system, we demonstrate high reaction rates for the enzymatic transesterification of Miglyol® oil 812 with methanol, catalyzed by Novozym® 435 in choline acetate/glycerol (1 : 1.5 molar ratio). The high conversion (97%) of the triglyceride obtained within 3 h, under optimal conditions, suggests that these novel eutectic solvents warrant further exploration as potential media in the enzymatic production of biodiesel. Text Antarc* Antarctica PubMed Central (PMC) Organic & Biomolecular Chemistry 9 6 1908 |
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Article Zhao, Hua Baker, Gary A. Holmes, Shaletha New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel† |
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description |
The enzymatic preparation of biodiesel has been hampered by the lack of suitable solvents with desirable properties such as high lipase compatibility, low cost, low viscosity, high biodegradability, and ease of product separation. Recent interest in using ionic liquids (ILs) as advanced reaction media has led to fast reaction rates and high yields in the enzymatic synthesis of biodiesel. However, conventional (i.e., cation–anion paired) ILs based on imidazolium and other quaternary ammonium salts remain too expensive for wide application at industrial scales. In this study, we report on newly-synthesized eutectic ILs derived from choline acetate or choline chloride coupled with biocompatible hydrogen-bond donors, such as glycerol. These eutectic solvents have favorable properties including low viscosity, high biodegradability, and excellent compatibility with Novozym® 435, a commercial immobilized Candida antarctica lipase B. Furthermore, in a model biodiesel synthesis system, we demonstrate high reaction rates for the enzymatic transesterification of Miglyol® oil 812 with methanol, catalyzed by Novozym® 435 in choline acetate/glycerol (1 : 1.5 molar ratio). The high conversion (97%) of the triglyceride obtained within 3 h, under optimal conditions, suggests that these novel eutectic solvents warrant further exploration as potential media in the enzymatic production of biodiesel. |
format |
Text |
author |
Zhao, Hua Baker, Gary A. Holmes, Shaletha |
author_facet |
Zhao, Hua Baker, Gary A. Holmes, Shaletha |
author_sort |
Zhao, Hua |
title |
New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel† |
title_short |
New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel† |
title_full |
New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel† |
title_fullStr |
New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel† |
title_full_unstemmed |
New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel† |
title_sort |
new eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel† |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416871 http://www.ncbi.nlm.nih.gov/pubmed/21283901 https://doi.org/10.1039/c0ob01011a |
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Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416871 http://www.ncbi.nlm.nih.gov/pubmed/21283901 http://dx.doi.org/10.1039/c0ob01011a |
op_rights |
© The Royal Society of Chemistry 2011 |
op_doi |
https://doi.org/10.1039/c0ob01011a |
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Organic & Biomolecular Chemistry |
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9 |
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6 |
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1908 |
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1766253123053027328 |