Enhancing Nitrification at Low Temperature with Zeolite in a Mining Operations Retention Pond
Ammonium nitrate explosives are used in mining operations at Diavik Diamond Mines Inc. in the Northwest Territories, Canada. Residual nitrogen is washed into the mine pit and piped to a nearby retention pond where its removal is accomplished by microbial activity prior to a final water treatment ste...
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ftpubmed:oai:pubmedcentral.nih.gov:3407968 2023-05-15T15:14:18+02:00 Enhancing Nitrification at Low Temperature with Zeolite in a Mining Operations Retention Pond Miazga-Rodriguez, Misha Han, Sukkyun Yakiwchuk, Brian Wei, Kai English, Colleen Bourn, Steven Bohnert, Seth Stein, Lisa Y. 2012-07-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407968 http://www.ncbi.nlm.nih.gov/pubmed/22866052 https://doi.org/10.3389/fmicb.2012.00271 en eng Frontiers Research Foundation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407968 http://www.ncbi.nlm.nih.gov/pubmed/22866052 http://dx.doi.org/10.3389/fmicb.2012.00271 Copyright © 2012 Miazga-Rodriguez, Han, Yakiwchuk, Wei, English, Bourn, Bohnert and Stein. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. CC-BY Microbiology Text 2012 ftpubmed https://doi.org/10.3389/fmicb.2012.00271 2013-09-04T10:47:53Z Ammonium nitrate explosives are used in mining operations at Diavik Diamond Mines Inc. in the Northwest Territories, Canada. Residual nitrogen is washed into the mine pit and piped to a nearby retention pond where its removal is accomplished by microbial activity prior to a final water treatment step and release into the sub-Arctic lake, Lac de Gras. Microbial removal of ammonium in the retention pond is rapid during the brief ice-free summer, but often slows under ice cover that persists up to 9 months of the year. The aluminosilicate mineral zeolite was tested as an additive to retention pond water to increase rates of ammonium removal at 4°C. Water samples were collected across the length of the retention pond monthly over a year. The structure of the microbial community (bacteria, archaea, and eukarya), as determined by denaturing gradient gel electrophoresis of PCR-amplified small subunit ribosomal RNA genes, was more stable during cold months than during July–September, when there was a marked phytoplankton bloom. Of the ammonia-oxidizing community, only bacterial amoA genes were consistently detected. Zeolite (10 g) was added to retention pond water (100 mL) amended with 5 mM ammonium and incubated at 12°C to encourage development of a nitrifying biofilm. The biofilm community was composed of different amoA phylotypes from those identified in gene clone libraries of native water samples. Zeolite biofilm was added to fresh water samples collected at different times of the year, resulting in a significant increase in laboratory measurements of potential nitrification activity at 4°C. A significant positive correlation between the amount of zeolite biofilm and potential nitrification activity was observed; rates were unaffected in incubations containing 1–20 mM ammonium. Addition of zeolite to retention ponds in cold environments could effectively increase nitrification rates year-round by concentrating active nitrifying biomass. Text Arctic Northwest Territories Phytoplankton PubMed Central (PMC) Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Canada Lac de Gras ENVELOPE(-110.501,-110.501,64.500,64.500) Northwest Territories Frontiers in Microbiology 3 |
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English |
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Microbiology |
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Microbiology Miazga-Rodriguez, Misha Han, Sukkyun Yakiwchuk, Brian Wei, Kai English, Colleen Bourn, Steven Bohnert, Seth Stein, Lisa Y. Enhancing Nitrification at Low Temperature with Zeolite in a Mining Operations Retention Pond |
topic_facet |
Microbiology |
description |
Ammonium nitrate explosives are used in mining operations at Diavik Diamond Mines Inc. in the Northwest Territories, Canada. Residual nitrogen is washed into the mine pit and piped to a nearby retention pond where its removal is accomplished by microbial activity prior to a final water treatment step and release into the sub-Arctic lake, Lac de Gras. Microbial removal of ammonium in the retention pond is rapid during the brief ice-free summer, but often slows under ice cover that persists up to 9 months of the year. The aluminosilicate mineral zeolite was tested as an additive to retention pond water to increase rates of ammonium removal at 4°C. Water samples were collected across the length of the retention pond monthly over a year. The structure of the microbial community (bacteria, archaea, and eukarya), as determined by denaturing gradient gel electrophoresis of PCR-amplified small subunit ribosomal RNA genes, was more stable during cold months than during July–September, when there was a marked phytoplankton bloom. Of the ammonia-oxidizing community, only bacterial amoA genes were consistently detected. Zeolite (10 g) was added to retention pond water (100 mL) amended with 5 mM ammonium and incubated at 12°C to encourage development of a nitrifying biofilm. The biofilm community was composed of different amoA phylotypes from those identified in gene clone libraries of native water samples. Zeolite biofilm was added to fresh water samples collected at different times of the year, resulting in a significant increase in laboratory measurements of potential nitrification activity at 4°C. A significant positive correlation between the amount of zeolite biofilm and potential nitrification activity was observed; rates were unaffected in incubations containing 1–20 mM ammonium. Addition of zeolite to retention ponds in cold environments could effectively increase nitrification rates year-round by concentrating active nitrifying biomass. |
format |
Text |
author |
Miazga-Rodriguez, Misha Han, Sukkyun Yakiwchuk, Brian Wei, Kai English, Colleen Bourn, Steven Bohnert, Seth Stein, Lisa Y. |
author_facet |
Miazga-Rodriguez, Misha Han, Sukkyun Yakiwchuk, Brian Wei, Kai English, Colleen Bourn, Steven Bohnert, Seth Stein, Lisa Y. |
author_sort |
Miazga-Rodriguez, Misha |
title |
Enhancing Nitrification at Low Temperature with Zeolite in a Mining Operations Retention Pond |
title_short |
Enhancing Nitrification at Low Temperature with Zeolite in a Mining Operations Retention Pond |
title_full |
Enhancing Nitrification at Low Temperature with Zeolite in a Mining Operations Retention Pond |
title_fullStr |
Enhancing Nitrification at Low Temperature with Zeolite in a Mining Operations Retention Pond |
title_full_unstemmed |
Enhancing Nitrification at Low Temperature with Zeolite in a Mining Operations Retention Pond |
title_sort |
enhancing nitrification at low temperature with zeolite in a mining operations retention pond |
publisher |
Frontiers Research Foundation |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407968 http://www.ncbi.nlm.nih.gov/pubmed/22866052 https://doi.org/10.3389/fmicb.2012.00271 |
long_lat |
ENVELOPE(-130.826,-130.826,57.231,57.231) ENVELOPE(-110.501,-110.501,64.500,64.500) |
geographic |
Arctic Arctic Lake Canada Lac de Gras Northwest Territories |
geographic_facet |
Arctic Arctic Lake Canada Lac de Gras Northwest Territories |
genre |
Arctic Northwest Territories Phytoplankton |
genre_facet |
Arctic Northwest Territories Phytoplankton |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407968 http://www.ncbi.nlm.nih.gov/pubmed/22866052 http://dx.doi.org/10.3389/fmicb.2012.00271 |
op_rights |
Copyright © 2012 Miazga-Rodriguez, Han, Yakiwchuk, Wei, English, Bourn, Bohnert and Stein. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2012.00271 |
container_title |
Frontiers in Microbiology |
container_volume |
3 |
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1766344768842891264 |