Identification of Surrogates of Protection against Yersiniosis in Immersion Vaccinated Atlantic Salmon
Simple cost-effective bacterins are the earliest and most successfully used commercial vaccines in fish. In particular, those prepared from Yersinia ruckeri have proven effective at controlling Enteric Red Mouth Disease (ERM) and yersiniosis in rainbow trout and Atlantic salmon, respectively. Howeve...
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ftpubmed:oai:pubmedcentral.nih.gov:3395641 2023-05-15T15:29:58+02:00 Identification of Surrogates of Protection against Yersiniosis in Immersion Vaccinated Atlantic Salmon Bridle, Andrew R. Koop, Ben F. Nowak, Barbara F. 2012-07-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395641 http://www.ncbi.nlm.nih.gov/pubmed/22808275 https://doi.org/10.1371/journal.pone.0040841 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395641 http://www.ncbi.nlm.nih.gov/pubmed/22808275 http://dx.doi.org/10.1371/journal.pone.0040841 Bridle et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2012 ftpubmed https://doi.org/10.1371/journal.pone.0040841 2013-09-04T10:01:56Z Simple cost-effective bacterins are the earliest and most successfully used commercial vaccines in fish. In particular, those prepared from Yersinia ruckeri have proven effective at controlling Enteric Red Mouth Disease (ERM) and yersiniosis in rainbow trout and Atlantic salmon, respectively. However, the emergence of outbreaks of ERM caused by atypical biotypes of Y. ruckeri and reports of vaccine failure resulting in mass mortality of hatchery Atlantic salmon has reinvigorated interest in vaccines against fish bacterial diseases. Therefore the objective of this study was to identify surrogates of protection against yersiniosis using cDNA microarray to characterise the response of host genes in the gills of unvaccinated and vaccinated Atlantic salmon challenged with Y. ruckeri. Differentially expressed genes were identified using two-way ANOVA and restricted to those with >2.5-fold change at P<0.05. Using cDNA microarray we identified the expression of 6 genes in response to infection and 4 genes associated with the protective host response to yersiniosis. Analysis by real-time PCR confirmed that three immunologically relevant genes, namely a cathelicidin (47-fold) and a C-type lectin (19-fold) increased in response to yersiniosis. Including collagenase (17-fold increase), an important tissue remodelling and repair enzyme, these genes represent 3 of 6 non-protective and/or pathological responses to yersiniosis. Genes associated with the protective host response included an immunoglobulin gene and a selenoprotein that showed significant fold changes (15-fold increases each), highlighting the importance of antibody-mediated protection against yersiniosis. These findings provide much needed knowledge of the host-pathogen interaction in response to bacterial infection and immunisation in fish. Significantly, we identified a transcriptional biosignature consisting of predominantly immune-relevant genes (14 up and 3 down-regulated) in the gills of Atlantic salmon after immersion vaccination and before bacterial ... Text Atlantic salmon PubMed Central (PMC) PLoS ONE 7 7 e40841 |
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Research Article Bridle, Andrew R. Koop, Ben F. Nowak, Barbara F. Identification of Surrogates of Protection against Yersiniosis in Immersion Vaccinated Atlantic Salmon |
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Research Article |
description |
Simple cost-effective bacterins are the earliest and most successfully used commercial vaccines in fish. In particular, those prepared from Yersinia ruckeri have proven effective at controlling Enteric Red Mouth Disease (ERM) and yersiniosis in rainbow trout and Atlantic salmon, respectively. However, the emergence of outbreaks of ERM caused by atypical biotypes of Y. ruckeri and reports of vaccine failure resulting in mass mortality of hatchery Atlantic salmon has reinvigorated interest in vaccines against fish bacterial diseases. Therefore the objective of this study was to identify surrogates of protection against yersiniosis using cDNA microarray to characterise the response of host genes in the gills of unvaccinated and vaccinated Atlantic salmon challenged with Y. ruckeri. Differentially expressed genes were identified using two-way ANOVA and restricted to those with >2.5-fold change at P<0.05. Using cDNA microarray we identified the expression of 6 genes in response to infection and 4 genes associated with the protective host response to yersiniosis. Analysis by real-time PCR confirmed that three immunologically relevant genes, namely a cathelicidin (47-fold) and a C-type lectin (19-fold) increased in response to yersiniosis. Including collagenase (17-fold increase), an important tissue remodelling and repair enzyme, these genes represent 3 of 6 non-protective and/or pathological responses to yersiniosis. Genes associated with the protective host response included an immunoglobulin gene and a selenoprotein that showed significant fold changes (15-fold increases each), highlighting the importance of antibody-mediated protection against yersiniosis. These findings provide much needed knowledge of the host-pathogen interaction in response to bacterial infection and immunisation in fish. Significantly, we identified a transcriptional biosignature consisting of predominantly immune-relevant genes (14 up and 3 down-regulated) in the gills of Atlantic salmon after immersion vaccination and before bacterial ... |
format |
Text |
author |
Bridle, Andrew R. Koop, Ben F. Nowak, Barbara F. |
author_facet |
Bridle, Andrew R. Koop, Ben F. Nowak, Barbara F. |
author_sort |
Bridle, Andrew R. |
title |
Identification of Surrogates of Protection against Yersiniosis in Immersion Vaccinated Atlantic Salmon |
title_short |
Identification of Surrogates of Protection against Yersiniosis in Immersion Vaccinated Atlantic Salmon |
title_full |
Identification of Surrogates of Protection against Yersiniosis in Immersion Vaccinated Atlantic Salmon |
title_fullStr |
Identification of Surrogates of Protection against Yersiniosis in Immersion Vaccinated Atlantic Salmon |
title_full_unstemmed |
Identification of Surrogates of Protection against Yersiniosis in Immersion Vaccinated Atlantic Salmon |
title_sort |
identification of surrogates of protection against yersiniosis in immersion vaccinated atlantic salmon |
publisher |
Public Library of Science |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395641 http://www.ncbi.nlm.nih.gov/pubmed/22808275 https://doi.org/10.1371/journal.pone.0040841 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395641 http://www.ncbi.nlm.nih.gov/pubmed/22808275 http://dx.doi.org/10.1371/journal.pone.0040841 |
op_rights |
Bridle et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0040841 |
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PLoS ONE |
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7 |
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7 |
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e40841 |
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