Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes
Methane (CH4) flux to the atmosphere is mitigated via microbial CH4 oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH4 emissions. We u...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
American Society for Microbiology
2012
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370501 http://www.ncbi.nlm.nih.gov/pubmed/22522690 https://doi.org/10.1128/AEM.00853-12 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:3370501 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:3370501 2023-05-15T14:51:58+02:00 Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes He, Ruo Wooller, Matthew J. Pohlman, John W. Quensen, John Tiedje, James M. Leigh, Mary Beth 2012-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370501 http://www.ncbi.nlm.nih.gov/pubmed/22522690 https://doi.org/10.1128/AEM.00853-12 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370501 http://www.ncbi.nlm.nih.gov/pubmed/22522690 http://dx.doi.org/10.1128/AEM.00853-12 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Microbial Ecology Text 2012 ftpubmed https://doi.org/10.1128/AEM.00853-12 2013-09-04T08:25:40Z Methane (CH4) flux to the atmosphere is mitigated via microbial CH4 oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH4 emissions. We used DNA-based stable-isotope probing (SIP), quantitative PCR (Q-PCR), and pyrosequencing analyses to identify and characterize methanotrophic communities active at a range of temperatures (4°C, 10°C, and 21°C) in sediments (to a depth of 25 cm) sampled from Lake Qalluuraq on the North Slope of Alaska. CH4 oxidation activity was measured in microcosm incubations containing sediments at all temperatures, with the highest CH4 oxidation potential of 37.5 μmol g−1 day−1 in the uppermost (depth, 0 to 1 cm) sediment at 21°C after 2 to 5 days of incubation. Q-PCR of pmoA and of the 16S rRNA genes of type I and type II methanotrophs, and pyrosequencing of 16S rRNA genes in 13C-labeled DNA obtained by SIP demonstrated that the type I methanotrophs Methylobacter, Methylomonas, and Methylosoma dominated carbon acquisition from CH4 in the sediments. The identity and relative abundance of active methanotrophs differed with the incubation temperature. Methylotrophs were also abundant in the microbial community that derived carbon from CH4, especially in the deeper sediments (depth, 15 to 20 cm) at low temperatures (4°C and 10°C), and showed a good linear relationship (R = 0.82) with the relative abundances of methanotrophs in pyrosequencing reads. This study describes for the first time how methanotrophic communities in arctic lake sediments respond to temperature variations. Text Arctic north slope Alaska PubMed Central (PMC) Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Applied and Environmental Microbiology 78 13 4715 4723 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology He, Ruo Wooller, Matthew J. Pohlman, John W. Quensen, John Tiedje, James M. Leigh, Mary Beth Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
topic_facet |
Microbial Ecology |
description |
Methane (CH4) flux to the atmosphere is mitigated via microbial CH4 oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH4 emissions. We used DNA-based stable-isotope probing (SIP), quantitative PCR (Q-PCR), and pyrosequencing analyses to identify and characterize methanotrophic communities active at a range of temperatures (4°C, 10°C, and 21°C) in sediments (to a depth of 25 cm) sampled from Lake Qalluuraq on the North Slope of Alaska. CH4 oxidation activity was measured in microcosm incubations containing sediments at all temperatures, with the highest CH4 oxidation potential of 37.5 μmol g−1 day−1 in the uppermost (depth, 0 to 1 cm) sediment at 21°C after 2 to 5 days of incubation. Q-PCR of pmoA and of the 16S rRNA genes of type I and type II methanotrophs, and pyrosequencing of 16S rRNA genes in 13C-labeled DNA obtained by SIP demonstrated that the type I methanotrophs Methylobacter, Methylomonas, and Methylosoma dominated carbon acquisition from CH4 in the sediments. The identity and relative abundance of active methanotrophs differed with the incubation temperature. Methylotrophs were also abundant in the microbial community that derived carbon from CH4, especially in the deeper sediments (depth, 15 to 20 cm) at low temperatures (4°C and 10°C), and showed a good linear relationship (R = 0.82) with the relative abundances of methanotrophs in pyrosequencing reads. This study describes for the first time how methanotrophic communities in arctic lake sediments respond to temperature variations. |
format |
Text |
author |
He, Ruo Wooller, Matthew J. Pohlman, John W. Quensen, John Tiedje, James M. Leigh, Mary Beth |
author_facet |
He, Ruo Wooller, Matthew J. Pohlman, John W. Quensen, John Tiedje, James M. Leigh, Mary Beth |
author_sort |
He, Ruo |
title |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_short |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_full |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_fullStr |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_full_unstemmed |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_sort |
shifts in identity and activity of methanotrophs in arctic lake sediments in response to temperature changes |
publisher |
American Society for Microbiology |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370501 http://www.ncbi.nlm.nih.gov/pubmed/22522690 https://doi.org/10.1128/AEM.00853-12 |
long_lat |
ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Arctic Arctic Lake |
geographic_facet |
Arctic Arctic Lake |
genre |
Arctic north slope Alaska |
genre_facet |
Arctic north slope Alaska |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370501 http://www.ncbi.nlm.nih.gov/pubmed/22522690 http://dx.doi.org/10.1128/AEM.00853-12 |
op_rights |
Copyright © 2012, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/AEM.00853-12 |
container_title |
Applied and Environmental Microbiology |
container_volume |
78 |
container_issue |
13 |
container_start_page |
4715 |
op_container_end_page |
4723 |
_version_ |
1766323097761218560 |