Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.)
Heart and skeletal inflammation (HSMI) of farmed Atlantic salmon (Salmo salar L.) is a disease characterized by a chronic myocarditis involving the epicardium and the compact and spongious part of the heart ventricle. Chronic myositis of the red skeletal muscle is also a typical finding of HSMI. Pis...
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ftpubmed:oai:pubmedcentral.nih.gov:3367920 2023-05-15T15:28:16+02:00 Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.) Mikalsen, Aase B. Haugland, Oyvind Rode, Marit Solbakk, Inge Tom Evensen, Oystein 2012-06-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367920 http://www.ncbi.nlm.nih.gov/pubmed/22693625 https://doi.org/10.1371/journal.pone.0037269 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367920 http://www.ncbi.nlm.nih.gov/pubmed/22693625 http://dx.doi.org/10.1371/journal.pone.0037269 Mikalsen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2012 ftpubmed https://doi.org/10.1371/journal.pone.0037269 2013-09-04T08:15:46Z Heart and skeletal inflammation (HSMI) of farmed Atlantic salmon (Salmo salar L.) is a disease characterized by a chronic myocarditis involving the epicardium and the compact and spongious part of the heart ventricle. Chronic myositis of the red skeletal muscle is also a typical finding of HSMI. Piscine reovirus (PRV) has been detected by real-time PCR from farmed and wild salmon with and without typical changes of HSMI and thus the causal relationship between presence of virus and the disease has not been fully determined [1]. In this study we show that the Atlantic salmon reovirus (ASRV), identical to PRV, can be passaged in GF-1 cells and experimental challenge of naïve Atlantic salmon with cell culture passaged reovirus results in cardiac and skeletal muscle pathology typical of HSMI with onset of pathology from 6 weeks, peaking by 9 weeks post challenge. ASRV replicates in heart tissue and the peak level of virus replication coincides with peak of heart lesions. We further demonstrate mRNA transcript assessment and in situ characterization that challenged fish develop a CD8+ T cell myocarditis. Text Atlantic salmon Salmo salar PubMed Central (PMC) PLoS ONE 7 6 e37269 |
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Research Article Mikalsen, Aase B. Haugland, Oyvind Rode, Marit Solbakk, Inge Tom Evensen, Oystein Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.) |
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Research Article |
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Heart and skeletal inflammation (HSMI) of farmed Atlantic salmon (Salmo salar L.) is a disease characterized by a chronic myocarditis involving the epicardium and the compact and spongious part of the heart ventricle. Chronic myositis of the red skeletal muscle is also a typical finding of HSMI. Piscine reovirus (PRV) has been detected by real-time PCR from farmed and wild salmon with and without typical changes of HSMI and thus the causal relationship between presence of virus and the disease has not been fully determined [1]. In this study we show that the Atlantic salmon reovirus (ASRV), identical to PRV, can be passaged in GF-1 cells and experimental challenge of naïve Atlantic salmon with cell culture passaged reovirus results in cardiac and skeletal muscle pathology typical of HSMI with onset of pathology from 6 weeks, peaking by 9 weeks post challenge. ASRV replicates in heart tissue and the peak level of virus replication coincides with peak of heart lesions. We further demonstrate mRNA transcript assessment and in situ characterization that challenged fish develop a CD8+ T cell myocarditis. |
format |
Text |
author |
Mikalsen, Aase B. Haugland, Oyvind Rode, Marit Solbakk, Inge Tom Evensen, Oystein |
author_facet |
Mikalsen, Aase B. Haugland, Oyvind Rode, Marit Solbakk, Inge Tom Evensen, Oystein |
author_sort |
Mikalsen, Aase B. |
title |
Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.) |
title_short |
Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.) |
title_full |
Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.) |
title_fullStr |
Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.) |
title_full_unstemmed |
Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.) |
title_sort |
atlantic salmon reovirus infection causes a cd8 t cell myocarditis in atlantic salmon (salmo salar l.) |
publisher |
Public Library of Science |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367920 http://www.ncbi.nlm.nih.gov/pubmed/22693625 https://doi.org/10.1371/journal.pone.0037269 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367920 http://www.ncbi.nlm.nih.gov/pubmed/22693625 http://dx.doi.org/10.1371/journal.pone.0037269 |
op_rights |
Mikalsen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0037269 |
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PLoS ONE |
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7 |
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6 |
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e37269 |
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1766358644882931712 |