Mining of unexplored habitats for novel chitinases—chiA as a helper gene proxy in metagenomics

• Published in: Applied Microbiology and Biotechnology
• Main Authors: Cretoiu, Mariana Silvia, Kielak, Anna Maria, Al-Soud, Waleed Abu, Sørensen, Søren J., van Elsas, Jan Dirk
• Format: Text
• Language: English
• Published: Springer-Verlag 2012
• Subjects: Methods and Protocols; Arctic; Oxyria digyna
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353111; http://www.ncbi.nlm.nih.gov/pubmed/22526805; https://doi.org/10.1007/s00253-012-4057-5

The main objective of this study was to assess the abundance and diversity of chitin-degrading microbial communities in ten terrestrial and aquatic habitats in order to provide guidance to the subsequent exploration of such environments for novel chitinolytic enzymes. A combined protocol which encompassed (1) classical overall enzymatic assays, (2) chiA gene abundance measurement by qPCR, (3) chiA gene pyrosequencing, and (4) chiA gene-based PCR-DGGE was used. The chiA gene pyrosequencing is unprecedented, as it is the first massive parallel sequencing of this gene. The data obtained showed the existence across habitats of core bacterial communities responsible for chitin assimilation irrespective of ecosystem origin. Conversely, there were habitat-specific differences. In addition, a suite of sequences were obtained that are as yet unregistered in the chitinase database. In terms of chiA gene abundance and diversity, typical low-abundance/diversity versus high-abundance/diversity habitats was distinguished. From the combined data, we selected chitin-amended agricultural soil, the rhizosphere of the Arctic plant Oxyria digyna and the freshwater sponge Ephydatia fluviatilis as the most promising habitats for subsequent bioexploration. Thus, the screening strategy used is proposed as a guide for further metagenomics-based exploration of the selected habitats.