LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence
Vibrio (Aliivibrio) salmonicida is the causal agent of cold-water vibriosis, a fatal bacterial septicemia primarily of farmed salmonid fish. The molecular mechanisms of invasion, colonization, and growth of V. salmonicida in the host are still largely unknown, and few virulence factors have been ide...
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ftpubmed:oai:pubmedcentral.nih.gov:3347430 2023-05-15T15:32:29+02:00 LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence Bjelland, Ane Mohn Sørum, Henning Tegegne, Daget Ayana Winther-Larsen, Hanne C. Willassen, Nils Peder Hansen, Hilde 2012-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347430 http://www.ncbi.nlm.nih.gov/pubmed/22371373 https://doi.org/10.1128/IAI.06038-11 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347430 http://www.ncbi.nlm.nih.gov/pubmed/22371373 http://dx.doi.org/10.1128/IAI.06038-11 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Text 2012 ftpubmed https://doi.org/10.1128/IAI.06038-11 2013-09-04T06:55:37Z Vibrio (Aliivibrio) salmonicida is the causal agent of cold-water vibriosis, a fatal bacterial septicemia primarily of farmed salmonid fish. The molecular mechanisms of invasion, colonization, and growth of V. salmonicida in the host are still largely unknown, and few virulence factors have been identified. Quorum sensing (QS) is a cell-to-cell communication system known to regulate virulence and other activities in several bacterial species. The genome of V. salmonicida LFI1238 encodes products presumably involved in several QS systems. In this study, the gene encoding LitR, a homolog of the master regulator of QS in V. fischeri, was deleted. Compared to the parental strain, the litR mutant showed increased motility, adhesion, cell-to-cell aggregation, and biofilm formation. Furthermore, the litR mutant produced less cryptic bioluminescence, whereas production of acylhomoserine lactones was unaffected. Our results also indicate a salinity-sensitive regulation of LitR. Finally, reduced mortality was observed in Atlantic salmon infected with the litR mutant, implying that the fish were more susceptible to infection with the wild type than with the mutant strain. We hypothesize that LitR inhibits biofilm formation and favors planktonic growth, with the latter being more adapted for pathogenesis in the fish host. Text Atlantic salmon PubMed Central (PMC) Infection and Immunity 80 5 1681 1689 |
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English |
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Cellular Microbiology: Pathogen-Host Cell Molecular Interactions |
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Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Bjelland, Ane Mohn Sørum, Henning Tegegne, Daget Ayana Winther-Larsen, Hanne C. Willassen, Nils Peder Hansen, Hilde LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
topic_facet |
Cellular Microbiology: Pathogen-Host Cell Molecular Interactions |
description |
Vibrio (Aliivibrio) salmonicida is the causal agent of cold-water vibriosis, a fatal bacterial septicemia primarily of farmed salmonid fish. The molecular mechanisms of invasion, colonization, and growth of V. salmonicida in the host are still largely unknown, and few virulence factors have been identified. Quorum sensing (QS) is a cell-to-cell communication system known to regulate virulence and other activities in several bacterial species. The genome of V. salmonicida LFI1238 encodes products presumably involved in several QS systems. In this study, the gene encoding LitR, a homolog of the master regulator of QS in V. fischeri, was deleted. Compared to the parental strain, the litR mutant showed increased motility, adhesion, cell-to-cell aggregation, and biofilm formation. Furthermore, the litR mutant produced less cryptic bioluminescence, whereas production of acylhomoserine lactones was unaffected. Our results also indicate a salinity-sensitive regulation of LitR. Finally, reduced mortality was observed in Atlantic salmon infected with the litR mutant, implying that the fish were more susceptible to infection with the wild type than with the mutant strain. We hypothesize that LitR inhibits biofilm formation and favors planktonic growth, with the latter being more adapted for pathogenesis in the fish host. |
format |
Text |
author |
Bjelland, Ane Mohn Sørum, Henning Tegegne, Daget Ayana Winther-Larsen, Hanne C. Willassen, Nils Peder Hansen, Hilde |
author_facet |
Bjelland, Ane Mohn Sørum, Henning Tegegne, Daget Ayana Winther-Larsen, Hanne C. Willassen, Nils Peder Hansen, Hilde |
author_sort |
Bjelland, Ane Mohn |
title |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_short |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_full |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_fullStr |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_full_unstemmed |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_sort |
litr of vibrio salmonicida is a salinity-sensitive quorum-sensing regulator of phenotypes involved in host interactions and virulence |
publisher |
American Society for Microbiology |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347430 http://www.ncbi.nlm.nih.gov/pubmed/22371373 https://doi.org/10.1128/IAI.06038-11 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347430 http://www.ncbi.nlm.nih.gov/pubmed/22371373 http://dx.doi.org/10.1128/IAI.06038-11 |
op_rights |
Copyright © 2012, American Society for Microbiology. All Rights Reserved. |
op_doi |
https://doi.org/10.1128/IAI.06038-11 |
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Infection and Immunity |
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80 |
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5 |
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1681 |
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1689 |
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1766362977912487936 |