Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2)
Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gig...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
MDPI
2012
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347016 http://www.ncbi.nlm.nih.gov/pubmed/22611355 https://doi.org/10.3390/md10030583 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:3347016 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:3347016 2023-05-15T15:57:39+02:00 Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) Mello, Danielle F. de Oliveira, Eliza S. Vieira, Renato C. Simoes, Erik Trevisan, Rafael Dafre, Alcir Luiz Barracco, Margherita Anna 2012-03-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347016 http://www.ncbi.nlm.nih.gov/pubmed/22611355 https://doi.org/10.3390/md10030583 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347016 http://www.ncbi.nlm.nih.gov/pubmed/22611355 http://dx.doi.org/10.3390/md10030583 © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). CC-BY Article Text 2012 ftpubmed https://doi.org/10.3390/md10030583 2013-09-04T06:54:03Z Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gigas hemocytes were exposed to brevetoxin (PbTx-2). Hemocyte viability, monitored through the neutral red retention and MTT reduction assays, and apoptosis (Hoechst staining) remained unchanged during 12 h of exposure to PbTx-2 in concentrations up to 1000 µg/L. Despite cell viability and apoptosis remained stable, hemocytes incubated for 4 h with 1000 µg/L of PbTx-2 revealed higher expression levels of Hsp70 (p < 0.01) and CYP356A1 (p < 0.05) transcripts and a tendency to increase FABP expression, as evaluated by Real-Time quantitative PCR. The expression of other studied genes (BPI, IL-17, GSTO, EcSOD, Prx6, SOD and GPx) remained unchanged. The results suggest that the absence of cytotoxic effects of PbTx-2 in Crassostrea gigas hemocytes, even at high concentrations, allow early defense responses to be produced by activating protective mechanisms associated to detoxification (CYP356A1 and possibly FABP) and stress (Hsp70), but not to immune or to antioxidant (BPI, IL-17, EcSOD, Prx6, GPx and SOD) related genes. Text Crassostrea gigas PubMed Central (PMC) Marine Drugs 10 12 583 597 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Mello, Danielle F. de Oliveira, Eliza S. Vieira, Renato C. Simoes, Erik Trevisan, Rafael Dafre, Alcir Luiz Barracco, Margherita Anna Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
topic_facet |
Article |
description |
Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gigas hemocytes were exposed to brevetoxin (PbTx-2). Hemocyte viability, monitored through the neutral red retention and MTT reduction assays, and apoptosis (Hoechst staining) remained unchanged during 12 h of exposure to PbTx-2 in concentrations up to 1000 µg/L. Despite cell viability and apoptosis remained stable, hemocytes incubated for 4 h with 1000 µg/L of PbTx-2 revealed higher expression levels of Hsp70 (p < 0.01) and CYP356A1 (p < 0.05) transcripts and a tendency to increase FABP expression, as evaluated by Real-Time quantitative PCR. The expression of other studied genes (BPI, IL-17, GSTO, EcSOD, Prx6, SOD and GPx) remained unchanged. The results suggest that the absence of cytotoxic effects of PbTx-2 in Crassostrea gigas hemocytes, even at high concentrations, allow early defense responses to be produced by activating protective mechanisms associated to detoxification (CYP356A1 and possibly FABP) and stress (Hsp70), but not to immune or to antioxidant (BPI, IL-17, EcSOD, Prx6, GPx and SOD) related genes. |
format |
Text |
author |
Mello, Danielle F. de Oliveira, Eliza S. Vieira, Renato C. Simoes, Erik Trevisan, Rafael Dafre, Alcir Luiz Barracco, Margherita Anna |
author_facet |
Mello, Danielle F. de Oliveira, Eliza S. Vieira, Renato C. Simoes, Erik Trevisan, Rafael Dafre, Alcir Luiz Barracco, Margherita Anna |
author_sort |
Mello, Danielle F. |
title |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_short |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_full |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_fullStr |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_full_unstemmed |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_sort |
cellular and transcriptional responses of crassostrea gigas hemocytes exposed in vitro to brevetoxin (pbtx-2) |
publisher |
MDPI |
publishDate |
2012 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347016 http://www.ncbi.nlm.nih.gov/pubmed/22611355 https://doi.org/10.3390/md10030583 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347016 http://www.ncbi.nlm.nih.gov/pubmed/22611355 http://dx.doi.org/10.3390/md10030583 |
op_rights |
© 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md10030583 |
container_title |
Marine Drugs |
container_volume |
10 |
container_issue |
12 |
container_start_page |
583 |
op_container_end_page |
597 |
_version_ |
1766393329903206400 |