Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway1[C][W][OA]
Dimethylsulfoniopropionate (DMSP) plays important roles in oceanic carbon and sulfur cycling and may significantly impact climate. It is a biomolecule synthesized from the methionine (Met) pathway and proposed to serve various physiological functions to aid in environmental stress adaptation through...
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ftpubmed:oai:pubmedcentral.nih.gov:3327215 2023-05-15T18:18:08+02:00 Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway1[C][W][OA] Lyon, Barbara R. Lee, Peter A. Bennett, Jennifer M. DiTullio, Giacomo R. Janech, Michael G. 2011-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327215 http://www.ncbi.nlm.nih.gov/pubmed/22034629 https://doi.org/10.1104/pp.111.185025 en eng American Society of Plant Biologists http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327215 http://www.ncbi.nlm.nih.gov/pubmed/22034629 http://dx.doi.org/10.1104/pp.111.185025 © 2011 American Society of Plant Biologists. All rights reserved. Environmental Stress and Adaptation to Stress Text 2011 ftpubmed https://doi.org/10.1104/pp.111.185025 2013-09-04T05:39:52Z Dimethylsulfoniopropionate (DMSP) plays important roles in oceanic carbon and sulfur cycling and may significantly impact climate. It is a biomolecule synthesized from the methionine (Met) pathway and proposed to serve various physiological functions to aid in environmental stress adaptation through its compatible solute, cryoprotectant, and antioxidant properties. Yet, the enzymes and mechanisms regulating DMSP production are poorly understood. This study utilized a proteomics approach to investigate protein changes associated with salinity-induced DMSP increases in the model sea-ice diatom Fragilariopsis cylindrus (CCMP 1102). We hypothesized proteins associated with the Met-DMSP biosynthesis pathway would increase in relative abundance when challenged with elevated salinity. To test this hypothesis axenic log-phase cultures initially grown at a salinity of 35 were gradually shifted to a final salinity of 70 over a 24-h period. Intracellular DMSP was measured and two-dimensional gel electrophoresis was used to identify protein changes at 48 h after the shift. Intracellular DMSP increased by approximately 85% in the hypersaline cultures. One-third of the proteins increased under high salinity were associated with amino acid pathways. Three protein isoforms of S-adenosylhomo-cysteine hydrolase, which synthesizes a Met precursor, increased 1.8- to 2.1-fold, two isoforms of S-adenosyl Met synthetase increased 1.9- to 2.5-fold, and S-adenosyl Met methyltransferase increased by 2.8-fold, suggesting active methyl cycle proteins are recruited in the synthesis of DMSP. Proteins from the four enzyme classes of the proposed algal Met transaminase DMSP pathway were among the elevated proteins, supporting our hypothesis and providing candidate genes for future characterization studies. Text Sea ice PubMed Central (PMC) Plant Physiology 157 4 1926 1941 |
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Environmental Stress and Adaptation to Stress |
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Environmental Stress and Adaptation to Stress Lyon, Barbara R. Lee, Peter A. Bennett, Jennifer M. DiTullio, Giacomo R. Janech, Michael G. Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway1[C][W][OA] |
topic_facet |
Environmental Stress and Adaptation to Stress |
description |
Dimethylsulfoniopropionate (DMSP) plays important roles in oceanic carbon and sulfur cycling and may significantly impact climate. It is a biomolecule synthesized from the methionine (Met) pathway and proposed to serve various physiological functions to aid in environmental stress adaptation through its compatible solute, cryoprotectant, and antioxidant properties. Yet, the enzymes and mechanisms regulating DMSP production are poorly understood. This study utilized a proteomics approach to investigate protein changes associated with salinity-induced DMSP increases in the model sea-ice diatom Fragilariopsis cylindrus (CCMP 1102). We hypothesized proteins associated with the Met-DMSP biosynthesis pathway would increase in relative abundance when challenged with elevated salinity. To test this hypothesis axenic log-phase cultures initially grown at a salinity of 35 were gradually shifted to a final salinity of 70 over a 24-h period. Intracellular DMSP was measured and two-dimensional gel electrophoresis was used to identify protein changes at 48 h after the shift. Intracellular DMSP increased by approximately 85% in the hypersaline cultures. One-third of the proteins increased under high salinity were associated with amino acid pathways. Three protein isoforms of S-adenosylhomo-cysteine hydrolase, which synthesizes a Met precursor, increased 1.8- to 2.1-fold, two isoforms of S-adenosyl Met synthetase increased 1.9- to 2.5-fold, and S-adenosyl Met methyltransferase increased by 2.8-fold, suggesting active methyl cycle proteins are recruited in the synthesis of DMSP. Proteins from the four enzyme classes of the proposed algal Met transaminase DMSP pathway were among the elevated proteins, supporting our hypothesis and providing candidate genes for future characterization studies. |
format |
Text |
author |
Lyon, Barbara R. Lee, Peter A. Bennett, Jennifer M. DiTullio, Giacomo R. Janech, Michael G. |
author_facet |
Lyon, Barbara R. Lee, Peter A. Bennett, Jennifer M. DiTullio, Giacomo R. Janech, Michael G. |
author_sort |
Lyon, Barbara R. |
title |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway1[C][W][OA] |
title_short |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway1[C][W][OA] |
title_full |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway1[C][W][OA] |
title_fullStr |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway1[C][W][OA] |
title_full_unstemmed |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway1[C][W][OA] |
title_sort |
proteomic analysis of a sea-ice diatom: salinity acclimation provides new insight into the dimethylsulfoniopropionate production pathway1[c][w][oa] |
publisher |
American Society of Plant Biologists |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327215 http://www.ncbi.nlm.nih.gov/pubmed/22034629 https://doi.org/10.1104/pp.111.185025 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327215 http://www.ncbi.nlm.nih.gov/pubmed/22034629 http://dx.doi.org/10.1104/pp.111.185025 |
op_rights |
© 2011 American Society of Plant Biologists. All rights reserved. |
op_doi |
https://doi.org/10.1104/pp.111.185025 |
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Plant Physiology |
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157 |
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4 |
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1926 |
op_container_end_page |
1941 |
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1766194568784510976 |