Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study
Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7×106 CFU/fish) but no to bath exposure. The i.p. c...
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ftpubmed:oai:pubmedcentral.nih.gov:3167283 2023-05-15T18:41:06+02:00 Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study Magi, G.E. Lopez-Romalde, S. Magariños, G.E. Lamas, J. Toranzo, A.E. Romalde, L. 2009-06-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167283 https://doi.org/10.4081/ejh.2009.e9 en eng PAGEPress Publications http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167283 http://dx.doi.org/10.4081/ejh.2009.e9 ©2009 European Journal of Histochemistry Original Paper Text 2009 ftpubmed https://doi.org/10.4081/ejh.2009.e9 2013-09-03T19:23:58Z Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7×106 CFU/fish) but no to bath exposure. The i.p. challenge induced septicaemic infection and mortality. Externally, moribund fish showed distended abdomen and pale areas at day 9. The gross pathological internal signs present were abundant ascitic fluid in the peritoneal cavity, pale and enlarged spleen, pale and friable liver, and congestive and dilated gut with yellowish exudates. On histopathological examination, bacterial invasion was common in all the tissues studied but the most prominent pathological changes were observed in gut, spleen and kidney after 7 day with features of necrosis. The immunohistochemical findings support the widespread localization of the bacteria after the i.p. injection since the P. anguilliseptica was detected in spleen from day 1 post injection, in liver, kidney and gut from day 4, in muscle from day 7 and in brain from day 9. The difficulties in infecting healthy fish by bath challenge can be explained by the opportunistic nature of this pathogen. Text Turbot PubMed Central (PMC) European Journal of Histochemistry 53 2 9 |
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Original Paper Magi, G.E. Lopez-Romalde, S. Magariños, G.E. Lamas, J. Toranzo, A.E. Romalde, L. Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
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Original Paper |
description |
Experimental infection with Pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (Psetta maxima) in order to evaluate the pathology induced. Turbot was found to be sensitive to i.p. challenge (1.7×106 CFU/fish) but no to bath exposure. The i.p. challenge induced septicaemic infection and mortality. Externally, moribund fish showed distended abdomen and pale areas at day 9. The gross pathological internal signs present were abundant ascitic fluid in the peritoneal cavity, pale and enlarged spleen, pale and friable liver, and congestive and dilated gut with yellowish exudates. On histopathological examination, bacterial invasion was common in all the tissues studied but the most prominent pathological changes were observed in gut, spleen and kidney after 7 day with features of necrosis. The immunohistochemical findings support the widespread localization of the bacteria after the i.p. injection since the P. anguilliseptica was detected in spleen from day 1 post injection, in liver, kidney and gut from day 4, in muscle from day 7 and in brain from day 9. The difficulties in infecting healthy fish by bath challenge can be explained by the opportunistic nature of this pathogen. |
format |
Text |
author |
Magi, G.E. Lopez-Romalde, S. Magariños, G.E. Lamas, J. Toranzo, A.E. Romalde, L. |
author_facet |
Magi, G.E. Lopez-Romalde, S. Magariños, G.E. Lamas, J. Toranzo, A.E. Romalde, L. |
author_sort |
Magi, G.E. |
title |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_short |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_full |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_fullStr |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_full_unstemmed |
Experimental Pseudomonas anguilliseptica infection in turbot Psetta maxima (L.): a histopathological and immunohistochemical study |
title_sort |
experimental pseudomonas anguilliseptica infection in turbot psetta maxima (l.): a histopathological and immunohistochemical study |
publisher |
PAGEPress Publications |
publishDate |
2009 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167283 https://doi.org/10.4081/ejh.2009.e9 |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167283 http://dx.doi.org/10.4081/ejh.2009.e9 |
op_rights |
©2009 European Journal of Histochemistry |
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https://doi.org/10.4081/ejh.2009.e9 |
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European Journal of Histochemistry |
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53 |
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2 |
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9 |
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1766230602358456320 |