CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom
Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B12 and carbon dioxide partial pressure (pCO2) influences growth rate, primary productivity, cellular...
Published in: | The ISME Journal |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
Nature Publishing Group
2011
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146264 http://www.ncbi.nlm.nih.gov/pubmed/21248860 https://doi.org/10.1038/ismej.2010.211 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:3146264 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:3146264 2023-05-15T18:28:16+02:00 CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom King, Andrew L Sañudo-Wilhelmy, Sergio A Leblanc, Karine Hutchins, David A Fu, Feixue 2011-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146264 http://www.ncbi.nlm.nih.gov/pubmed/21248860 https://doi.org/10.1038/ismej.2010.211 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146264 http://www.ncbi.nlm.nih.gov/pubmed/21248860 http://dx.doi.org/10.1038/ismej.2010.211 Copyright © 2011 International Society for Microbial Ecology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ CC-BY-NC-ND Original Article Text 2011 ftpubmed https://doi.org/10.1038/ismej.2010.211 2013-09-03T18:00:45Z Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B12 and carbon dioxide partial pressure (pCO2) influences growth rate, primary productivity, cellular iron (Fe), cobalt (Co), zinc (Zn) and cadmium (Cd) quotas, and the net use efficiencies (NUEs) of these bioactive trace metals (mol C fixed per mol cellular trace metal per day). Under B12-replete conditions, cells grown at high pCO2 had lower Fe, Zn and Cd quotas, and used those trace metals more efficiently in comparison with cells grown at low pCO2. At high pCO2, B12-limited cells had ∼50% lower specific growth and carbon fixation rates, and used Fe ∼15-fold less efficiently, and Zn and Cd ∼3-fold less efficiently, in comparison with B12-replete cells. The observed higher Fe, Zn and Cd NUE under high pCO2/B12-replete conditions are consistent with predicted downregulation of carbon-concentrating mechanisms. Co quotas of B12-replete cells were ∼5- to 14-fold higher in comparison with B12-limited cells, suggesting that >80% of cellular Co of B12-limited cells was likely from B12. Our results demonstrate that CO2 and vitamin B12 interactively influence growth, carbon fixation, trace metal requirements and trace metal NUE of this diatom. This suggests the need to consider complex feedback interactions between multiple environmental factors for this biogeochemically critical group of phytoplankton in the last glacial maximum as well as the current and future changing ocean. Text Subarctic PubMed Central (PMC) Pacific The ISME Journal 5 8 1388 1396 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Original Article |
spellingShingle |
Original Article King, Andrew L Sañudo-Wilhelmy, Sergio A Leblanc, Karine Hutchins, David A Fu, Feixue CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
topic_facet |
Original Article |
description |
Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B12 and carbon dioxide partial pressure (pCO2) influences growth rate, primary productivity, cellular iron (Fe), cobalt (Co), zinc (Zn) and cadmium (Cd) quotas, and the net use efficiencies (NUEs) of these bioactive trace metals (mol C fixed per mol cellular trace metal per day). Under B12-replete conditions, cells grown at high pCO2 had lower Fe, Zn and Cd quotas, and used those trace metals more efficiently in comparison with cells grown at low pCO2. At high pCO2, B12-limited cells had ∼50% lower specific growth and carbon fixation rates, and used Fe ∼15-fold less efficiently, and Zn and Cd ∼3-fold less efficiently, in comparison with B12-replete cells. The observed higher Fe, Zn and Cd NUE under high pCO2/B12-replete conditions are consistent with predicted downregulation of carbon-concentrating mechanisms. Co quotas of B12-replete cells were ∼5- to 14-fold higher in comparison with B12-limited cells, suggesting that >80% of cellular Co of B12-limited cells was likely from B12. Our results demonstrate that CO2 and vitamin B12 interactively influence growth, carbon fixation, trace metal requirements and trace metal NUE of this diatom. This suggests the need to consider complex feedback interactions between multiple environmental factors for this biogeochemically critical group of phytoplankton in the last glacial maximum as well as the current and future changing ocean. |
format |
Text |
author |
King, Andrew L Sañudo-Wilhelmy, Sergio A Leblanc, Karine Hutchins, David A Fu, Feixue |
author_facet |
King, Andrew L Sañudo-Wilhelmy, Sergio A Leblanc, Karine Hutchins, David A Fu, Feixue |
author_sort |
King, Andrew L |
title |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_short |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_full |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_fullStr |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_full_unstemmed |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_sort |
co2 and vitamin b12 interactions determine bioactive trace metal requirements of a subarctic pacific diatom |
publisher |
Nature Publishing Group |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146264 http://www.ncbi.nlm.nih.gov/pubmed/21248860 https://doi.org/10.1038/ismej.2010.211 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146264 http://www.ncbi.nlm.nih.gov/pubmed/21248860 http://dx.doi.org/10.1038/ismej.2010.211 |
op_rights |
Copyright © 2011 International Society for Microbial Ecology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1038/ismej.2010.211 |
container_title |
The ISME Journal |
container_volume |
5 |
container_issue |
8 |
container_start_page |
1388 |
op_container_end_page |
1396 |
_version_ |
1766210668035309568 |