Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing ▿ †
Arctic soils are increasingly susceptible to petroleum hydrocarbon contamination, as exploration and exploitation of the Arctic increase. Bioremediation in these soils is challenging due to logistical constraints and because soil temperatures only rise above 0°C for ∼2 months each year. Nitrogen is...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131650 http://www.ncbi.nlm.nih.gov/pubmed/21498745 https://doi.org/10.1128/AEM.00172-11 |
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ftpubmed:oai:pubmedcentral.nih.gov:3131650 2023-05-15T14:55:34+02:00 Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing ▿ † Bell, Terrence H. Yergeau, Etienne Martineau, Christine Juck, David Whyte, Lyle G. Greer, Charles W. 2011-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131650 http://www.ncbi.nlm.nih.gov/pubmed/21498745 https://doi.org/10.1128/AEM.00172-11 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131650 http://www.ncbi.nlm.nih.gov/pubmed/21498745 http://dx.doi.org/10.1128/AEM.00172-11 Copyright © 2011, American Society for Microbiology Biodegradation Text 2011 ftpubmed https://doi.org/10.1128/AEM.00172-11 2013-09-03T17:03:40Z Arctic soils are increasingly susceptible to petroleum hydrocarbon contamination, as exploration and exploitation of the Arctic increase. Bioremediation in these soils is challenging due to logistical constraints and because soil temperatures only rise above 0°C for ∼2 months each year. Nitrogen is often added to contaminated soil in situ to stimulate the existing microbial community, but little is known about how the added nutrients are used by these microorganisms. Microbes vary widely in their ability to metabolize petroleum hydrocarbons, so the question becomes: which hydrocarbon-degrading microorganisms most effectively use this added nitrogen for growth? Using [15N]DNA-based stable isotope probing, we determined which taxonomic groups most readily incorporated nitrogen from the monoammonium phosphate added to contaminated and uncontaminated soil in Canadian Forces Station-Alert, Nunavut, Canada. Fractions from each sample were amplified with bacterial 16S rRNA and alkane monooxygenase B (alkB) gene-specific primers and then sequenced using lage-scale parallel-pyrosequencing. Sequence data was combined with 16S rRNA and alkB gene C quantitative PCR data to measure the presence of various phylogenetic groups in fractions at different buoyant densities. Several families of Proteobacteria and Actinobacteria that are directly involved in petroleum degradation incorporated the added nitrogen in contaminated soils, but it was the DNA of Sphingomonadaceae that was most enriched in 15N. Bacterial growth in uncontaminated soils was not stimulated by nutrient amendment. Our results suggest that nitrogen uptake efficiency differs between bacterial groups in contaminated soils. A better understanding of how groups of hydrocarbon-degraders contribute to the catabolism of petroleum will facilitate the design of more targeted bioremediation treatments. Text Arctic Nunavut PubMed Central (PMC) Arctic Nunavut Canada Canadian Forces Station Alert ENVELOPE(-62.350,-62.350,82.499,82.499) Applied and Environmental Microbiology 77 12 4163 4171 |
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Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Biodegradation |
spellingShingle |
Biodegradation Bell, Terrence H. Yergeau, Etienne Martineau, Christine Juck, David Whyte, Lyle G. Greer, Charles W. Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing ▿ † |
topic_facet |
Biodegradation |
description |
Arctic soils are increasingly susceptible to petroleum hydrocarbon contamination, as exploration and exploitation of the Arctic increase. Bioremediation in these soils is challenging due to logistical constraints and because soil temperatures only rise above 0°C for ∼2 months each year. Nitrogen is often added to contaminated soil in situ to stimulate the existing microbial community, but little is known about how the added nutrients are used by these microorganisms. Microbes vary widely in their ability to metabolize petroleum hydrocarbons, so the question becomes: which hydrocarbon-degrading microorganisms most effectively use this added nitrogen for growth? Using [15N]DNA-based stable isotope probing, we determined which taxonomic groups most readily incorporated nitrogen from the monoammonium phosphate added to contaminated and uncontaminated soil in Canadian Forces Station-Alert, Nunavut, Canada. Fractions from each sample were amplified with bacterial 16S rRNA and alkane monooxygenase B (alkB) gene-specific primers and then sequenced using lage-scale parallel-pyrosequencing. Sequence data was combined with 16S rRNA and alkB gene C quantitative PCR data to measure the presence of various phylogenetic groups in fractions at different buoyant densities. Several families of Proteobacteria and Actinobacteria that are directly involved in petroleum degradation incorporated the added nitrogen in contaminated soils, but it was the DNA of Sphingomonadaceae that was most enriched in 15N. Bacterial growth in uncontaminated soils was not stimulated by nutrient amendment. Our results suggest that nitrogen uptake efficiency differs between bacterial groups in contaminated soils. A better understanding of how groups of hydrocarbon-degraders contribute to the catabolism of petroleum will facilitate the design of more targeted bioremediation treatments. |
format |
Text |
author |
Bell, Terrence H. Yergeau, Etienne Martineau, Christine Juck, David Whyte, Lyle G. Greer, Charles W. |
author_facet |
Bell, Terrence H. Yergeau, Etienne Martineau, Christine Juck, David Whyte, Lyle G. Greer, Charles W. |
author_sort |
Bell, Terrence H. |
title |
Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing ▿ † |
title_short |
Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing ▿ † |
title_full |
Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing ▿ † |
title_fullStr |
Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing ▿ † |
title_full_unstemmed |
Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing ▿ † |
title_sort |
identification of nitrogen-incorporating bacteria in petroleum-contaminated arctic soils by using [15n]dna-based stable isotope probing and pyrosequencing ▿ † |
publisher |
American Society for Microbiology |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131650 http://www.ncbi.nlm.nih.gov/pubmed/21498745 https://doi.org/10.1128/AEM.00172-11 |
long_lat |
ENVELOPE(-62.350,-62.350,82.499,82.499) |
geographic |
Arctic Nunavut Canada Canadian Forces Station Alert |
geographic_facet |
Arctic Nunavut Canada Canadian Forces Station Alert |
genre |
Arctic Nunavut |
genre_facet |
Arctic Nunavut |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131650 http://www.ncbi.nlm.nih.gov/pubmed/21498745 http://dx.doi.org/10.1128/AEM.00172-11 |
op_rights |
Copyright © 2011, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.00172-11 |
container_title |
Applied and Environmental Microbiology |
container_volume |
77 |
container_issue |
12 |
container_start_page |
4163 |
op_container_end_page |
4171 |
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1766327603636994048 |