Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantiosele...
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Molecular Diversity Preservation International
2011
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111190 http://www.ncbi.nlm.nih.gov/pubmed/21673897 https://doi.org/10.3390/md9050889 |
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ftpubmed:oai:pubmedcentral.nih.gov:3111190 2023-05-15T13:35:52+02:00 Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey Araújo, Lidiane S. Kagohara, Edna Garcia, Thaís P. Pellizari, Vivian H. Andrade, Leandro H. 2011-05-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111190 http://www.ncbi.nlm.nih.gov/pubmed/21673897 https://doi.org/10.3390/md9050889 en eng Molecular Diversity Preservation International http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111190 http://www.ncbi.nlm.nih.gov/pubmed/21673897 http://dx.doi.org/10.3390/md9050889 © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). CC-BY Article Text 2011 ftpubmed https://doi.org/10.3390/md9050889 2013-09-03T15:43:49Z Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph. Text Antarc* Antarctic Antarctic Peninsula PubMed Central (PMC) Antarctic Antarctic Peninsula Marine Drugs 9 5 889 905 |
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Article Araújo, Lidiane S. Kagohara, Edna Garcia, Thaís P. Pellizari, Vivian H. Andrade, Leandro H. Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
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description |
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph. |
format |
Text |
author |
Araújo, Lidiane S. Kagohara, Edna Garcia, Thaís P. Pellizari, Vivian H. Andrade, Leandro H. |
author_facet |
Araújo, Lidiane S. Kagohara, Edna Garcia, Thaís P. Pellizari, Vivian H. Andrade, Leandro H. |
author_sort |
Araújo, Lidiane S. |
title |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_short |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_full |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_fullStr |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_full_unstemmed |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_sort |
screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey |
publisher |
Molecular Diversity Preservation International |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111190 http://www.ncbi.nlm.nih.gov/pubmed/21673897 https://doi.org/10.3390/md9050889 |
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Antarctic Antarctic Peninsula |
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Antarctic Antarctic Peninsula |
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Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111190 http://www.ncbi.nlm.nih.gov/pubmed/21673897 http://dx.doi.org/10.3390/md9050889 |
op_rights |
© 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md9050889 |
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Marine Drugs |
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905 |
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