Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba, Canada
The region of Churchill, Manitoba, contains a wide variety of habitats representative of both the boreal forest and arctic tundra and has been used as a model site for biodiversity studies for nearly seven decades within Canada. Much previous work has been done in Churchill to study the Daphnia pule...
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ftpubmed:oai:pubmedcentral.nih.gov:3096620 2023-05-15T14:59:11+02:00 Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba, Canada Jeffery, Nicholas W. Elías-Gutiérrez, Manuel Adamowicz, Sarah J. 2011-05-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096620 http://www.ncbi.nlm.nih.gov/pubmed/21610864 https://doi.org/10.1371/journal.pone.0018364 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096620 http://www.ncbi.nlm.nih.gov/pubmed/21610864 http://dx.doi.org/10.1371/journal.pone.0018364 Jeffery et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2011 ftpubmed https://doi.org/10.1371/journal.pone.0018364 2013-09-03T14:47:10Z The region of Churchill, Manitoba, contains a wide variety of habitats representative of both the boreal forest and arctic tundra and has been used as a model site for biodiversity studies for nearly seven decades within Canada. Much previous work has been done in Churchill to study the Daphnia pulex species complex in particular, but no study has completed a wide-scale survey on the crustacean species that inhabit Churchill's aquatic ecosystems using molecular markers. We have employed DNA barcoding to study the diversity of the Branchiopoda (Crustacea) in a wide variety of freshwater habitats and to determine the likely origins of the Churchill fauna following the last glaciation. The standard animal barcode marker (COI) was sequenced for 327 specimens, and a 3% divergence threshold was used to delineate potential species. We found 42 provisional and valid branchiopod species from this survey alone, including several cryptic lineages, in comparison with the 25 previously recorded from previous ecological works. Using published sequence data, we explored the phylogeographic affinities of Churchill's branchiopods, finding that the Churchill fauna apparently originated from all directions from multiple glacial refugia (including southern, Beringian, and high arctic regions). Overall, these microcrustaceans are very diverse in Churchill and contain multiple species complexes. The present study introduces among the first sequences for some understudied genera, for which further work is required to delineate species boundaries and develop a more complete understanding of branchiopod diversity over a larger spatial scale. Text Arctic Churchill Tundra PubMed Central (PMC) Arctic Canada PLoS ONE 6 5 e18364 |
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Research Article |
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Research Article Jeffery, Nicholas W. Elías-Gutiérrez, Manuel Adamowicz, Sarah J. Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba, Canada |
topic_facet |
Research Article |
description |
The region of Churchill, Manitoba, contains a wide variety of habitats representative of both the boreal forest and arctic tundra and has been used as a model site for biodiversity studies for nearly seven decades within Canada. Much previous work has been done in Churchill to study the Daphnia pulex species complex in particular, but no study has completed a wide-scale survey on the crustacean species that inhabit Churchill's aquatic ecosystems using molecular markers. We have employed DNA barcoding to study the diversity of the Branchiopoda (Crustacea) in a wide variety of freshwater habitats and to determine the likely origins of the Churchill fauna following the last glaciation. The standard animal barcode marker (COI) was sequenced for 327 specimens, and a 3% divergence threshold was used to delineate potential species. We found 42 provisional and valid branchiopod species from this survey alone, including several cryptic lineages, in comparison with the 25 previously recorded from previous ecological works. Using published sequence data, we explored the phylogeographic affinities of Churchill's branchiopods, finding that the Churchill fauna apparently originated from all directions from multiple glacial refugia (including southern, Beringian, and high arctic regions). Overall, these microcrustaceans are very diverse in Churchill and contain multiple species complexes. The present study introduces among the first sequences for some understudied genera, for which further work is required to delineate species boundaries and develop a more complete understanding of branchiopod diversity over a larger spatial scale. |
format |
Text |
author |
Jeffery, Nicholas W. Elías-Gutiérrez, Manuel Adamowicz, Sarah J. |
author_facet |
Jeffery, Nicholas W. Elías-Gutiérrez, Manuel Adamowicz, Sarah J. |
author_sort |
Jeffery, Nicholas W. |
title |
Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba, Canada |
title_short |
Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba, Canada |
title_full |
Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba, Canada |
title_fullStr |
Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba, Canada |
title_full_unstemmed |
Species Diversity and Phylogeographical Affinities of the Branchiopoda (Crustacea) of Churchill, Manitoba, Canada |
title_sort |
species diversity and phylogeographical affinities of the branchiopoda (crustacea) of churchill, manitoba, canada |
publisher |
Public Library of Science |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096620 http://www.ncbi.nlm.nih.gov/pubmed/21610864 https://doi.org/10.1371/journal.pone.0018364 |
geographic |
Arctic Canada |
geographic_facet |
Arctic Canada |
genre |
Arctic Churchill Tundra |
genre_facet |
Arctic Churchill Tundra |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096620 http://www.ncbi.nlm.nih.gov/pubmed/21610864 http://dx.doi.org/10.1371/journal.pone.0018364 |
op_rights |
Jeffery et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0018364 |
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PLoS ONE |
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6 |
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5 |
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e18364 |
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