Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica
The diversity and cold-active hydrolytic enzymes of culturable bacteria associated with sandy sediment from Nella Fjord, Eastern Antarctica (69°22′6″ S, 76°21′45″ E) was investigated. A total of 33 aerobic heterotrophic bacterial strains were isolated at 4 °C. These bacterial isolates could be sorte...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
MDPI
2011
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093252 http://www.ncbi.nlm.nih.gov/pubmed/21566794 https://doi.org/10.3390/md9020184 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:3093252 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:3093252 2023-05-15T13:54:36+02:00 Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica Yu, Yong Li, Hui-Rong Zeng, Yin-Xin Chen, Bo 2011-01-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093252 http://www.ncbi.nlm.nih.gov/pubmed/21566794 https://doi.org/10.3390/md9020184 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093252 http://www.ncbi.nlm.nih.gov/pubmed/21566794 http://dx.doi.org/10.3390/md9020184 © 2011 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). CC-BY Article Text 2011 ftpubmed https://doi.org/10.3390/md9020184 2013-09-03T14:32:13Z The diversity and cold-active hydrolytic enzymes of culturable bacteria associated with sandy sediment from Nella Fjord, Eastern Antarctica (69°22′6″ S, 76°21′45″ E) was investigated. A total of 33 aerobic heterotrophic bacterial strains were isolated at 4 °C. These bacterial isolates could be sorted into 18 phylotypes based on the 16S rRNA gene sequence belonging to four phyla, namely Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria. Only seven isolates were psychrophilic, 15 isolates were moderately psychrophilic, and 11 isolates were psychrotolerant. More than 72% of the isolates required sodium chloride to grow. Esterase, β-glucosidase and proteases activities at 4 °C were detected in more than 45% of the strains while approximately 21%, 15% and 12% of the strains possessed lipase, amylase and chitinase, respectively. These results indicate that a relatively high culturable bacterial diversity is present within marine sediment of Nella Fjord and it could serve as an ideal candidate region for bioprospecting. Text Antarc* Antarctica PubMed Central (PMC) Nella Fjord ENVELOPE(76.350,76.350,-69.375,-69.375) Marine Drugs 9 2 184 195 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Yu, Yong Li, Hui-Rong Zeng, Yin-Xin Chen, Bo Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica |
topic_facet |
Article |
description |
The diversity and cold-active hydrolytic enzymes of culturable bacteria associated with sandy sediment from Nella Fjord, Eastern Antarctica (69°22′6″ S, 76°21′45″ E) was investigated. A total of 33 aerobic heterotrophic bacterial strains were isolated at 4 °C. These bacterial isolates could be sorted into 18 phylotypes based on the 16S rRNA gene sequence belonging to four phyla, namely Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria. Only seven isolates were psychrophilic, 15 isolates were moderately psychrophilic, and 11 isolates were psychrotolerant. More than 72% of the isolates required sodium chloride to grow. Esterase, β-glucosidase and proteases activities at 4 °C were detected in more than 45% of the strains while approximately 21%, 15% and 12% of the strains possessed lipase, amylase and chitinase, respectively. These results indicate that a relatively high culturable bacterial diversity is present within marine sediment of Nella Fjord and it could serve as an ideal candidate region for bioprospecting. |
format |
Text |
author |
Yu, Yong Li, Hui-Rong Zeng, Yin-Xin Chen, Bo |
author_facet |
Yu, Yong Li, Hui-Rong Zeng, Yin-Xin Chen, Bo |
author_sort |
Yu, Yong |
title |
Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica |
title_short |
Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica |
title_full |
Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica |
title_fullStr |
Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica |
title_full_unstemmed |
Bacterial Diversity and Bioprospecting for Cold-Active Hydrolytic Enzymes from Culturable Bacteria Associated with Sediment from Nella Fjord, Eastern Antarctica |
title_sort |
bacterial diversity and bioprospecting for cold-active hydrolytic enzymes from culturable bacteria associated with sediment from nella fjord, eastern antarctica |
publisher |
MDPI |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093252 http://www.ncbi.nlm.nih.gov/pubmed/21566794 https://doi.org/10.3390/md9020184 |
long_lat |
ENVELOPE(76.350,76.350,-69.375,-69.375) |
geographic |
Nella Fjord |
geographic_facet |
Nella Fjord |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093252 http://www.ncbi.nlm.nih.gov/pubmed/21566794 http://dx.doi.org/10.3390/md9020184 |
op_rights |
© 2011 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md9020184 |
container_title |
Marine Drugs |
container_volume |
9 |
container_issue |
2 |
container_start_page |
184 |
op_container_end_page |
195 |
_version_ |
1766260583271759872 |