ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport
Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has...
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ftpubmed:oai:pubmedcentral.nih.gov:3052256 2023-05-15T16:06:33+02:00 ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloïse North, Helen M. Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Mylène 2011-02-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052256 http://www.ncbi.nlm.nih.gov/pubmed/21408051 https://doi.org/10.1371/journal.pone.0016645 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052256 http://www.ncbi.nlm.nih.gov/pubmed/21408051 http://dx.doi.org/10.1371/journal.pone.0016645 Lefebvre et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2011 ftpubmed https://doi.org/10.1371/journal.pone.0016645 2013-09-03T11:54:54Z Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has been described as involved in freezing, cold and salt tolerance as well as in water economy: esk1 mutants have very low evapo-transpiration rates and high water-use efficiency. In order to establish ESK1 function, detailed characterization of esk1 mutants has been carried out. The stress hormone ABA (abscisic acid) was present at high levels in esk1 compared to wild type, nevertheless, the weak water loss of esk1 was independent of stomata closure through ABA biosynthesis, as combining mutant in this pathway with esk1 led to additive phenotypes. Measurement of root hydraulic conductivity suggests that the esk1 vegetative apparatus suffers water deficit due to a defect in water transport. ESK1 promoter-driven reporter gene expression was observed in xylem and fibers, the vascular tissue responsible for the transport of water and mineral nutrients from the soil to the shoots, via the roots. Moreover, in cross sections of hypocotyls, roots and stems, esk1 xylem vessels were collapsed. Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants. Taken together our findings show that ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components. Text eskimo* PubMed Central (PMC) PLoS ONE 6 2 e16645 |
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Research Article Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloïse North, Helen M. Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Mylène ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
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Research Article |
description |
Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has been described as involved in freezing, cold and salt tolerance as well as in water economy: esk1 mutants have very low evapo-transpiration rates and high water-use efficiency. In order to establish ESK1 function, detailed characterization of esk1 mutants has been carried out. The stress hormone ABA (abscisic acid) was present at high levels in esk1 compared to wild type, nevertheless, the weak water loss of esk1 was independent of stomata closure through ABA biosynthesis, as combining mutant in this pathway with esk1 led to additive phenotypes. Measurement of root hydraulic conductivity suggests that the esk1 vegetative apparatus suffers water deficit due to a defect in water transport. ESK1 promoter-driven reporter gene expression was observed in xylem and fibers, the vascular tissue responsible for the transport of water and mineral nutrients from the soil to the shoots, via the roots. Moreover, in cross sections of hypocotyls, roots and stems, esk1 xylem vessels were collapsed. Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants. Taken together our findings show that ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components. |
format |
Text |
author |
Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloïse North, Helen M. Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Mylène |
author_facet |
Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloïse North, Helen M. Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Mylène |
author_sort |
Lefebvre, Valérie |
title |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_short |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_full |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_fullStr |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_full_unstemmed |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_sort |
eskimo1 disruption in arabidopsis alters vascular tissue and impairs water transport |
publisher |
Public Library of Science |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052256 http://www.ncbi.nlm.nih.gov/pubmed/21408051 https://doi.org/10.1371/journal.pone.0016645 |
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eskimo* |
genre_facet |
eskimo* |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052256 http://www.ncbi.nlm.nih.gov/pubmed/21408051 http://dx.doi.org/10.1371/journal.pone.0016645 |
op_rights |
Lefebvre et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0016645 |
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PLoS ONE |
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6 |
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2 |
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e16645 |
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