Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada▿
Shiga-toxigenic Escherichia coli (STEC) is an important cause of diarrheal disease. The most notorious STEC serotype is O157:H7, which is associated with hemorrhagic colitis and hemolytic-uremic syndrome (HUS). As a result, this serotype is routinely screened for in clinical microbiology laboratorie...
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ftpubmed:oai:pubmedcentral.nih.gov:3043486 2023-05-15T17:46:44+02:00 Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada▿ Couturier, Marc Roger Lee, Bonita Zelyas, Nathan Chui, Linda 2011-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3043486 http://www.ncbi.nlm.nih.gov/pubmed/21147949 https://doi.org/10.1128/JCM.01693-10 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3043486 http://www.ncbi.nlm.nih.gov/pubmed/21147949 http://dx.doi.org/10.1128/JCM.01693-10 Copyright © 2011, American Society for Microbiology. Epidemiology Text 2011 ftpubmed https://doi.org/10.1128/JCM.01693-10 2013-09-03T11:21:48Z Shiga-toxigenic Escherichia coli (STEC) is an important cause of diarrheal disease. The most notorious STEC serotype is O157:H7, which is associated with hemorrhagic colitis and hemolytic-uremic syndrome (HUS). As a result, this serotype is routinely screened for in clinical microbiology laboratories. With the bias toward the identification of the O157 serogroup in routine diagnostic processes, non-O157 STEC has been largely underrepresented in the epidemiology of STEC infections. This diagnostic bias is further complicated by the fact that many non-O157 STEC infections cause nonspecific gastroenteritis symptoms reminiscent of enteric viral infections. In this study, real-time PCR was used to amplify Shiga toxin genetic determinants (stx1 and stx2) from enriched stool samples that were initially submitted for the testing of enteric viruses in patients with suspected viral gastroenteritis between May and September of 2006, 2007, and 2008 (n = 2,702). Samples were submitted from the province of Alberta, Yukon, the Northwest Territories, and Nunavut, Canada. A total of 38 samples (1.4%) tested positive for Shiga toxin genes, and 15 isolates were cultured for further characterization. Several of the serotypes identified (O157:H7, O26:HNM, O26:H11, O103:H25, O121:H19, and O145:HNM) have been previously associated with outbreaks and HUS. This study outlines the importance of combining molecular methods with classical culture techniques to enhance the detection of emerging non-O157 as well as O157 serotypes in diarrheal stool samples. Furthermore, atypical diarrhea disease caused by non-O157 STEC can be routinely missed due to screening only for viral agents. Text Northwest Territories Nunavut Yukon PubMed Central (PMC) Canada Northwest Territories Nunavut Yukon Journal of Clinical Microbiology 49 2 574 578 |
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Epidemiology Couturier, Marc Roger Lee, Bonita Zelyas, Nathan Chui, Linda Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada▿ |
topic_facet |
Epidemiology |
description |
Shiga-toxigenic Escherichia coli (STEC) is an important cause of diarrheal disease. The most notorious STEC serotype is O157:H7, which is associated with hemorrhagic colitis and hemolytic-uremic syndrome (HUS). As a result, this serotype is routinely screened for in clinical microbiology laboratories. With the bias toward the identification of the O157 serogroup in routine diagnostic processes, non-O157 STEC has been largely underrepresented in the epidemiology of STEC infections. This diagnostic bias is further complicated by the fact that many non-O157 STEC infections cause nonspecific gastroenteritis symptoms reminiscent of enteric viral infections. In this study, real-time PCR was used to amplify Shiga toxin genetic determinants (stx1 and stx2) from enriched stool samples that were initially submitted for the testing of enteric viruses in patients with suspected viral gastroenteritis between May and September of 2006, 2007, and 2008 (n = 2,702). Samples were submitted from the province of Alberta, Yukon, the Northwest Territories, and Nunavut, Canada. A total of 38 samples (1.4%) tested positive for Shiga toxin genes, and 15 isolates were cultured for further characterization. Several of the serotypes identified (O157:H7, O26:HNM, O26:H11, O103:H25, O121:H19, and O145:HNM) have been previously associated with outbreaks and HUS. This study outlines the importance of combining molecular methods with classical culture techniques to enhance the detection of emerging non-O157 as well as O157 serotypes in diarrheal stool samples. Furthermore, atypical diarrhea disease caused by non-O157 STEC can be routinely missed due to screening only for viral agents. |
format |
Text |
author |
Couturier, Marc Roger Lee, Bonita Zelyas, Nathan Chui, Linda |
author_facet |
Couturier, Marc Roger Lee, Bonita Zelyas, Nathan Chui, Linda |
author_sort |
Couturier, Marc Roger |
title |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada▿ |
title_short |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada▿ |
title_full |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada▿ |
title_fullStr |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada▿ |
title_full_unstemmed |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada▿ |
title_sort |
shiga-toxigenic escherichia coli detection in stool samples screened for viral gastroenteritis in alberta, canada▿ |
publisher |
American Society for Microbiology |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3043486 http://www.ncbi.nlm.nih.gov/pubmed/21147949 https://doi.org/10.1128/JCM.01693-10 |
geographic |
Canada Northwest Territories Nunavut Yukon |
geographic_facet |
Canada Northwest Territories Nunavut Yukon |
genre |
Northwest Territories Nunavut Yukon |
genre_facet |
Northwest Territories Nunavut Yukon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3043486 http://www.ncbi.nlm.nih.gov/pubmed/21147949 http://dx.doi.org/10.1128/JCM.01693-10 |
op_rights |
Copyright © 2011, American Society for Microbiology. |
op_doi |
https://doi.org/10.1128/JCM.01693-10 |
container_title |
Journal of Clinical Microbiology |
container_volume |
49 |
container_issue |
2 |
container_start_page |
574 |
op_container_end_page |
578 |
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1766150551974707200 |