Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection

Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typi...

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Published in:Veterinary Research
Main Authors: Løvoll, Marie, Austbø, Lars, Jørgensen, Jorunn B, Rimstad, Espen, Frost, Petter
Format: Text
Language:English
Published: BioMed Central 2011
Subjects:
Short Report
Atlantic salmon
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031228
http://www.ncbi.nlm.nih.gov/pubmed/21314970
https://doi.org/10.1186/1297-9716-42-8
id ftpubmed:oai:pubmedcentral.nih.gov:3031228
record_format openpolar
spelling ftpubmed:oai:pubmedcentral.nih.gov:3031228 2023-05-15T15:30:27+02:00 Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection Løvoll, Marie Austbø, Lars Jørgensen, Jorunn B Rimstad, Espen Frost, Petter 2011 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031228 http://www.ncbi.nlm.nih.gov/pubmed/21314970 https://doi.org/10.1186/1297-9716-42-8 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031228 http://www.ncbi.nlm.nih.gov/pubmed/21314970 http://dx.doi.org/10.1186/1297-9716-42-8 Copyright ©2011 Løvoll et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Short Report Text 2011 ftpubmed https://doi.org/10.1186/1297-9716-42-8 2013-09-03T10:35:56Z Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typically used reference genes in Atlantic salmon is not stable during experimental infection with salmon pancreas disease virus (SPDV). Transcript levels 0 to 6 weeks after challenge revealed statistically significant changes between time-points that corresponded with a peak in viral load 3 weeks after challenge. The results emphasize the need for thorough method validation prior to transcriptional studies during viral infections. Text Atlantic salmon PubMed Central (PMC) Veterinary Research 42 1 8
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Short Report
spellingShingle Short Report
Løvoll, Marie
Austbø, Lars
Jørgensen, Jorunn B
Rimstad, Espen
Frost, Petter
Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
topic_facet Short Report
description Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typically used reference genes in Atlantic salmon is not stable during experimental infection with salmon pancreas disease virus (SPDV). Transcript levels 0 to 6 weeks after challenge revealed statistically significant changes between time-points that corresponded with a peak in viral load 3 weeks after challenge. The results emphasize the need for thorough method validation prior to transcriptional studies during viral infections.
format Text
author Løvoll, Marie
Austbø, Lars
Jørgensen, Jorunn B
Rimstad, Espen
Frost, Petter
author_facet Løvoll, Marie
Austbø, Lars
Jørgensen, Jorunn B
Rimstad, Espen
Frost, Petter
author_sort Løvoll, Marie
title Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
title_short Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
title_full Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
title_fullStr Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
title_full_unstemmed Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
title_sort transcription of reference genes used for quantitative rt-pcr in atlantic salmon is affected by viral infection
publisher BioMed Central
publishDate 2011
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031228
http://www.ncbi.nlm.nih.gov/pubmed/21314970
https://doi.org/10.1186/1297-9716-42-8
genre Atlantic salmon
genre_facet Atlantic salmon
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031228
http://www.ncbi.nlm.nih.gov/pubmed/21314970
http://dx.doi.org/10.1186/1297-9716-42-8
op_rights Copyright ©2011 Løvoll et al; licensee BioMed Central Ltd.
http://creativecommons.org/licenses/by/2.0
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
op_rightsnorm CC-BY
op_doi https://doi.org/10.1186/1297-9716-42-8
container_title Veterinary Research
container_volume 42
container_issue 1
container_start_page 8
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