Recombination Within and Between Species of the Alpha Proteobacterium Bartonella Infecting Rodents
Bartonella infections from wild mice and voles (Apodemus flavicollis, Mi. oeconomus, Microtus arvalis and Myodes glareolus) were sampled from a forest and old-field habitats of eastern Poland; a complex network of Bartonella isolates, referrable to B. taylorii, B. grahamii, B. birtlesii and B. doshi...
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ftpubmed:oai:pubmedcentral.nih.gov:3011088 2023-05-15T17:12:37+02:00 Recombination Within and Between Species of the Alpha Proteobacterium Bartonella Infecting Rodents Paziewska, Anna Harris, Philip D. Zwolińska, Lucyna Bajer, Anna Siński, Edward 2010-08-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011088 http://www.ncbi.nlm.nih.gov/pubmed/20740281 https://doi.org/10.1007/s00248-010-9735-1 en eng Springer-Verlag http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011088 http://www.ncbi.nlm.nih.gov/pubmed/20740281 http://dx.doi.org/10.1007/s00248-010-9735-1 © The Author(s) 2010 Genes and Genomes Text 2010 ftpubmed https://doi.org/10.1007/s00248-010-9735-1 2013-09-03T09:20:44Z Bartonella infections from wild mice and voles (Apodemus flavicollis, Mi. oeconomus, Microtus arvalis and Myodes glareolus) were sampled from a forest and old-field habitats of eastern Poland; a complex network of Bartonella isolates, referrable to B. taylorii, B. grahamii, B. birtlesii and B. doshiae, was identified by the sequencing of a gltA fragment, comparable to previous studies of Bartonella diversity in rodents. Nested clade analysis showed that isolates could be assigned to zero- and one-step clades which correlated with host identity and were probably the result of clonal expansion; however, sequencing of other housekeeping genes (rpoB, ribC, ftsZ, groEl) and the 16S RNA gene revealed a more complex situation with clear evidence of numerous recombinant events in which one or both Bartonella parents could be identified. Recombination within gltA was found to have generated two distinct variant clades, one a hybrid between B. taylorii and B. doshiae, the other between B. taylorii and B. grahamii. These recombinant events characterised the differences between the two-step and higher clades within the total nested cladogram, involved all four species of Bartonella identified in this work and appear to have played a dominant role in the evolution of Bartonella diversity. It is clear, therefore, that housekeeping gene phylogenies are not robust indicators of Bartonella diversity, especially when only a single gene (gltA or 16S RNA) is used. Bartonella clades infecting Microtus were most frequently involved in recombination and were most frequently tip clades within the cladogram. The role of Microtus in influencing the frequency of Bartonella recombination remains unknown. Text Microtus arvalis PubMed Central (PMC) Microbial Ecology 61 1 134 145 |
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language |
English |
topic |
Genes and Genomes |
spellingShingle |
Genes and Genomes Paziewska, Anna Harris, Philip D. Zwolińska, Lucyna Bajer, Anna Siński, Edward Recombination Within and Between Species of the Alpha Proteobacterium Bartonella Infecting Rodents |
topic_facet |
Genes and Genomes |
description |
Bartonella infections from wild mice and voles (Apodemus flavicollis, Mi. oeconomus, Microtus arvalis and Myodes glareolus) were sampled from a forest and old-field habitats of eastern Poland; a complex network of Bartonella isolates, referrable to B. taylorii, B. grahamii, B. birtlesii and B. doshiae, was identified by the sequencing of a gltA fragment, comparable to previous studies of Bartonella diversity in rodents. Nested clade analysis showed that isolates could be assigned to zero- and one-step clades which correlated with host identity and were probably the result of clonal expansion; however, sequencing of other housekeeping genes (rpoB, ribC, ftsZ, groEl) and the 16S RNA gene revealed a more complex situation with clear evidence of numerous recombinant events in which one or both Bartonella parents could be identified. Recombination within gltA was found to have generated two distinct variant clades, one a hybrid between B. taylorii and B. doshiae, the other between B. taylorii and B. grahamii. These recombinant events characterised the differences between the two-step and higher clades within the total nested cladogram, involved all four species of Bartonella identified in this work and appear to have played a dominant role in the evolution of Bartonella diversity. It is clear, therefore, that housekeeping gene phylogenies are not robust indicators of Bartonella diversity, especially when only a single gene (gltA or 16S RNA) is used. Bartonella clades infecting Microtus were most frequently involved in recombination and were most frequently tip clades within the cladogram. The role of Microtus in influencing the frequency of Bartonella recombination remains unknown. |
format |
Text |
author |
Paziewska, Anna Harris, Philip D. Zwolińska, Lucyna Bajer, Anna Siński, Edward |
author_facet |
Paziewska, Anna Harris, Philip D. Zwolińska, Lucyna Bajer, Anna Siński, Edward |
author_sort |
Paziewska, Anna |
title |
Recombination Within and Between Species of the Alpha Proteobacterium Bartonella Infecting Rodents |
title_short |
Recombination Within and Between Species of the Alpha Proteobacterium Bartonella Infecting Rodents |
title_full |
Recombination Within and Between Species of the Alpha Proteobacterium Bartonella Infecting Rodents |
title_fullStr |
Recombination Within and Between Species of the Alpha Proteobacterium Bartonella Infecting Rodents |
title_full_unstemmed |
Recombination Within and Between Species of the Alpha Proteobacterium Bartonella Infecting Rodents |
title_sort |
recombination within and between species of the alpha proteobacterium bartonella infecting rodents |
publisher |
Springer-Verlag |
publishDate |
2010 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011088 http://www.ncbi.nlm.nih.gov/pubmed/20740281 https://doi.org/10.1007/s00248-010-9735-1 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011088 http://www.ncbi.nlm.nih.gov/pubmed/20740281 http://dx.doi.org/10.1007/s00248-010-9735-1 |
op_rights |
© The Author(s) 2010 |
op_doi |
https://doi.org/10.1007/s00248-010-9735-1 |
container_title |
Microbial Ecology |
container_volume |
61 |
container_issue |
1 |
container_start_page |
134 |
op_container_end_page |
145 |
_version_ |
1766069405855252480 |