High-level expression of sperm whale myoglobin in Escherichia coli.

• Main Authors: Springer, B A, Sligar, S G
• Format: Text
• Language: English
• Published: 1987
• Subjects: Research Article; Holo; Sperm whale
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC299671; http://www.ncbi.nlm.nih.gov/pubmed/3321062

Sperm whale myoglobin was expressed in Escherichia coli from a totally synthetic gene inserted in the expression vector pUC19. The gene was constructed as 23 overlapping oligonucleotides encoding both strands of the DNA. Gene synthesis provides several advantages over traditional eukaryotic gene-clo...