Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters
Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected su...
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ftpubmed:oai:pubmedcentral.nih.gov:2946586 2023-05-15T17:44:37+02:00 Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters Broman, T. Thelaus, J. Andersson, A.-C. Bäckman, S. Wikström, P. Larsson, E. Granberg, M. Karlsson, L. Bäck, E. Eliasson, H. Mattsson, R. Sjöstedt, A. Forsman, M. 2011 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946586 http://www.ncbi.nlm.nih.gov/pubmed/20885922 https://doi.org/10.1155/2011/851946 en eng Hindawi Publishing Corporation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946586 http://www.ncbi.nlm.nih.gov/pubmed/20885922 http://dx.doi.org/10.1155/2011/851946 Copyright © 2011 T. Broman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Article Text 2011 ftpubmed https://doi.org/10.1155/2011/851946 2013-09-03T05:18:57Z Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected surface water samples (n = 341) and sediment samples (n = 245) in two areas in Sweden with endemic tularemia. Real-time PCR screening demonstrated the presence of F. tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively. The 16S rRNA sequences from those samples all grouped to the species F. tularensis. Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F. tularensis subspecies holarctica-specific sequences. These sequences were detected in water sampled during both outbreak and nonoutbreak years. Our results indicate that diverse F. tularensis-like organisms, including F. tularensis subsp. holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia. Text Northern Sweden PubMed Central (PMC) International Journal of Microbiology 2011 1 10 |
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Research Article Broman, T. Thelaus, J. Andersson, A.-C. Bäckman, S. Wikström, P. Larsson, E. Granberg, M. Karlsson, L. Bäck, E. Eliasson, H. Mattsson, R. Sjöstedt, A. Forsman, M. Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
topic_facet |
Research Article |
description |
Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected surface water samples (n = 341) and sediment samples (n = 245) in two areas in Sweden with endemic tularemia. Real-time PCR screening demonstrated the presence of F. tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively. The 16S rRNA sequences from those samples all grouped to the species F. tularensis. Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F. tularensis subspecies holarctica-specific sequences. These sequences were detected in water sampled during both outbreak and nonoutbreak years. Our results indicate that diverse F. tularensis-like organisms, including F. tularensis subsp. holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia. |
format |
Text |
author |
Broman, T. Thelaus, J. Andersson, A.-C. Bäckman, S. Wikström, P. Larsson, E. Granberg, M. Karlsson, L. Bäck, E. Eliasson, H. Mattsson, R. Sjöstedt, A. Forsman, M. |
author_facet |
Broman, T. Thelaus, J. Andersson, A.-C. Bäckman, S. Wikström, P. Larsson, E. Granberg, M. Karlsson, L. Bäck, E. Eliasson, H. Mattsson, R. Sjöstedt, A. Forsman, M. |
author_sort |
Broman, T. |
title |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_short |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_full |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_fullStr |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_full_unstemmed |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_sort |
molecular detection of persistent francisella tularensis subspecies holarctica in natural waters |
publisher |
Hindawi Publishing Corporation |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946586 http://www.ncbi.nlm.nih.gov/pubmed/20885922 https://doi.org/10.1155/2011/851946 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946586 http://www.ncbi.nlm.nih.gov/pubmed/20885922 http://dx.doi.org/10.1155/2011/851946 |
op_rights |
Copyright © 2011 T. Broman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1155/2011/851946 |
container_title |
International Journal of Microbiology |
container_volume |
2011 |
container_start_page |
1 |
op_container_end_page |
10 |
_version_ |
1766146863263645696 |