Transgenesis in Xenopus using the Sleeping Beauty transposon system
Transposon-based integration systems have been widely used for genetic manipulation of invertebrate and plant model systems. In the past decade, these powerful tools have begun to be used in vertebrates for transgenesis, insertional mutagenesis and gene therapy applications. Sleeping Beauty (SB) is...
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ftpubmed:oai:pubmedcentral.nih.gov:2848081 2023-05-15T15:32:08+02:00 Transgenesis in Xenopus using the Sleeping Beauty transposon system Yergeau, Donald A. Johnson Hamlet, Michelle R. Kuliyev, Emin Zhu, Haiqing Doherty, Joanne R. Archer, Taylor D. Subhawong, Andrea P. Valentine, Marc B. Kelley, Clair M. Mead, Paul E. 2009-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848081 http://www.ncbi.nlm.nih.gov/pubmed/19517568 https://doi.org/10.1002/dvdy.21994 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848081 http://www.ncbi.nlm.nih.gov/pubmed/19517568 http://dx.doi.org/10.1002/dvdy.21994 Article Text 2009 ftpubmed https://doi.org/10.1002/dvdy.21994 2013-09-02T23:05:20Z Transposon-based integration systems have been widely used for genetic manipulation of invertebrate and plant model systems. In the past decade, these powerful tools have begun to be used in vertebrates for transgenesis, insertional mutagenesis and gene therapy applications. Sleeping Beauty (SB) is a member of Tc1/mariner class of transposases and is derived from an inactive form of the gene isolated from Atlantic salmon. SB has been used extensively in human cell lines and in whole animal vertebrate model systems such as the mouse, rat and zebrafish. In this study, we describe the use of SB in the diploid frog Xenopus tropicalis to generate stable transgenic lines. SB transposon transgenes integrate into the X. tropicalis genome by a non-canonical process and are passed through the germline. We compare the activity of SB in this model organism with that of Tol2, a hAT (hobo, Ac1, TAM)-like transposon system. Text Atlantic salmon PubMed Central (PMC) Developmental Dynamics 238 7 1727 1743 |
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Article Yergeau, Donald A. Johnson Hamlet, Michelle R. Kuliyev, Emin Zhu, Haiqing Doherty, Joanne R. Archer, Taylor D. Subhawong, Andrea P. Valentine, Marc B. Kelley, Clair M. Mead, Paul E. Transgenesis in Xenopus using the Sleeping Beauty transposon system |
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Transposon-based integration systems have been widely used for genetic manipulation of invertebrate and plant model systems. In the past decade, these powerful tools have begun to be used in vertebrates for transgenesis, insertional mutagenesis and gene therapy applications. Sleeping Beauty (SB) is a member of Tc1/mariner class of transposases and is derived from an inactive form of the gene isolated from Atlantic salmon. SB has been used extensively in human cell lines and in whole animal vertebrate model systems such as the mouse, rat and zebrafish. In this study, we describe the use of SB in the diploid frog Xenopus tropicalis to generate stable transgenic lines. SB transposon transgenes integrate into the X. tropicalis genome by a non-canonical process and are passed through the germline. We compare the activity of SB in this model organism with that of Tol2, a hAT (hobo, Ac1, TAM)-like transposon system. |
format |
Text |
author |
Yergeau, Donald A. Johnson Hamlet, Michelle R. Kuliyev, Emin Zhu, Haiqing Doherty, Joanne R. Archer, Taylor D. Subhawong, Andrea P. Valentine, Marc B. Kelley, Clair M. Mead, Paul E. |
author_facet |
Yergeau, Donald A. Johnson Hamlet, Michelle R. Kuliyev, Emin Zhu, Haiqing Doherty, Joanne R. Archer, Taylor D. Subhawong, Andrea P. Valentine, Marc B. Kelley, Clair M. Mead, Paul E. |
author_sort |
Yergeau, Donald A. |
title |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_short |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_full |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_fullStr |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_full_unstemmed |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_sort |
transgenesis in xenopus using the sleeping beauty transposon system |
publishDate |
2009 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848081 http://www.ncbi.nlm.nih.gov/pubmed/19517568 https://doi.org/10.1002/dvdy.21994 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848081 http://www.ncbi.nlm.nih.gov/pubmed/19517568 http://dx.doi.org/10.1002/dvdy.21994 |
op_doi |
https://doi.org/10.1002/dvdy.21994 |
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Developmental Dynamics |
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238 |
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7 |
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1727 |
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1743 |
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1766362644957102080 |