Quantitative Assessment of Ammonia-Oxidizing Bacterial Communities in the Epiphyton of Submerged Macrophytes in Shallow Lakes▿

In addition to the benthic and pelagic habitats, the epiphytic compartment of submerged macrophytes in shallow freshwater lakes offers a niche to bacterial ammonia-oxidizing communities. However, the diversity, numbers, and activity of epiphytic ammonia-oxidizing bacteria have long been overlooked....

Full description

Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Coci, M., Nicol, G. W., Pilloni, G. N., Schmid, M., Kamst-van Agterveld, M. P., Bodelier, P. L. E., Laanbroek, H. J.
Format: Text
Language:English
Published: American Society for Microbiology (ASM) 2010
Subjects:
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838014
http://www.ncbi.nlm.nih.gov/pubmed/20097811
https://doi.org/10.1128/AEM.01917-09
id ftpubmed:oai:pubmedcentral.nih.gov:2838014
record_format openpolar
spelling ftpubmed:oai:pubmedcentral.nih.gov:2838014 2023-05-15T15:53:55+02:00 Quantitative Assessment of Ammonia-Oxidizing Bacterial Communities in the Epiphyton of Submerged Macrophytes in Shallow Lakes▿ Coci, M. Nicol, G. W. Pilloni, G. N. Schmid, M. Kamst-van Agterveld, M. P. Bodelier, P. L. E. Laanbroek, H. J. 2010-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838014 http://www.ncbi.nlm.nih.gov/pubmed/20097811 https://doi.org/10.1128/AEM.01917-09 en eng American Society for Microbiology (ASM) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838014 http://www.ncbi.nlm.nih.gov/pubmed/20097811 http://dx.doi.org/10.1128/AEM.01917-09 Copyright © 2010, American Society for Microbiology Microbial Ecology Text 2010 ftpubmed https://doi.org/10.1128/AEM.01917-09 2013-09-02T22:29:24Z In addition to the benthic and pelagic habitats, the epiphytic compartment of submerged macrophytes in shallow freshwater lakes offers a niche to bacterial ammonia-oxidizing communities. However, the diversity, numbers, and activity of epiphytic ammonia-oxidizing bacteria have long been overlooked. In the present study, we analyzed quantitatively the epiphytic communities of three shallow lakes by a potential nitrification assay and by quantitative PCR of 16S rRNA genes. On the basis of the m2 of the lake surface, the gene copy numbers of epiphytic ammonia oxidizers were not significantly different from those in the benthic and pelagic compartments. The potential ammonia-oxidizing activities measured in the epiphytic compartment were also not significantly different from the activities determined in the benthic compartment. No potential ammonia-oxidizing activities were observed in the pelagic compartment. No activity was detected in the epiphyton of Chara aspera, the dominant submerged macrophyte in Lake Nuldernauw in The Netherlands. The presence of ammonia-oxidizing bacterial cells in the epiphyton of Potamogeton pectinatus was also demonstrated by fluorescent in situ hybridization microscopy images. By comparing the community composition as assessed by the 16S rRNA gene PCR-denaturing gradient gel electrophoresis approach, it was concluded that the epiphytic ammonia-oxidizing communities consisted of cells that were also present in the benthic and pelagic compartments. Of the environmental parameters examined, only the water retention time, the Kjeldahl nitrogen content, and the total phosphorus content correlated with potential ammonia-oxidizing activities. None of these parameters correlated with the numbers of gene copies related to ammonia-oxidizing betaproteobacteria. Text Chara aspera PubMed Central (PMC) Applied and Environmental Microbiology 76 6 1813 1821
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Microbial Ecology
spellingShingle Microbial Ecology
Coci, M.
Nicol, G. W.
Pilloni, G. N.
Schmid, M.
Kamst-van Agterveld, M. P.
Bodelier, P. L. E.
Laanbroek, H. J.
Quantitative Assessment of Ammonia-Oxidizing Bacterial Communities in the Epiphyton of Submerged Macrophytes in Shallow Lakes▿
topic_facet Microbial Ecology
description In addition to the benthic and pelagic habitats, the epiphytic compartment of submerged macrophytes in shallow freshwater lakes offers a niche to bacterial ammonia-oxidizing communities. However, the diversity, numbers, and activity of epiphytic ammonia-oxidizing bacteria have long been overlooked. In the present study, we analyzed quantitatively the epiphytic communities of three shallow lakes by a potential nitrification assay and by quantitative PCR of 16S rRNA genes. On the basis of the m2 of the lake surface, the gene copy numbers of epiphytic ammonia oxidizers were not significantly different from those in the benthic and pelagic compartments. The potential ammonia-oxidizing activities measured in the epiphytic compartment were also not significantly different from the activities determined in the benthic compartment. No potential ammonia-oxidizing activities were observed in the pelagic compartment. No activity was detected in the epiphyton of Chara aspera, the dominant submerged macrophyte in Lake Nuldernauw in The Netherlands. The presence of ammonia-oxidizing bacterial cells in the epiphyton of Potamogeton pectinatus was also demonstrated by fluorescent in situ hybridization microscopy images. By comparing the community composition as assessed by the 16S rRNA gene PCR-denaturing gradient gel electrophoresis approach, it was concluded that the epiphytic ammonia-oxidizing communities consisted of cells that were also present in the benthic and pelagic compartments. Of the environmental parameters examined, only the water retention time, the Kjeldahl nitrogen content, and the total phosphorus content correlated with potential ammonia-oxidizing activities. None of these parameters correlated with the numbers of gene copies related to ammonia-oxidizing betaproteobacteria.
format Text
author Coci, M.
Nicol, G. W.
Pilloni, G. N.
Schmid, M.
Kamst-van Agterveld, M. P.
Bodelier, P. L. E.
Laanbroek, H. J.
author_facet Coci, M.
Nicol, G. W.
Pilloni, G. N.
Schmid, M.
Kamst-van Agterveld, M. P.
Bodelier, P. L. E.
Laanbroek, H. J.
author_sort Coci, M.
title Quantitative Assessment of Ammonia-Oxidizing Bacterial Communities in the Epiphyton of Submerged Macrophytes in Shallow Lakes▿
title_short Quantitative Assessment of Ammonia-Oxidizing Bacterial Communities in the Epiphyton of Submerged Macrophytes in Shallow Lakes▿
title_full Quantitative Assessment of Ammonia-Oxidizing Bacterial Communities in the Epiphyton of Submerged Macrophytes in Shallow Lakes▿
title_fullStr Quantitative Assessment of Ammonia-Oxidizing Bacterial Communities in the Epiphyton of Submerged Macrophytes in Shallow Lakes▿
title_full_unstemmed Quantitative Assessment of Ammonia-Oxidizing Bacterial Communities in the Epiphyton of Submerged Macrophytes in Shallow Lakes▿
title_sort quantitative assessment of ammonia-oxidizing bacterial communities in the epiphyton of submerged macrophytes in shallow lakes▿
publisher American Society for Microbiology (ASM)
publishDate 2010
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838014
http://www.ncbi.nlm.nih.gov/pubmed/20097811
https://doi.org/10.1128/AEM.01917-09
genre Chara aspera
genre_facet Chara aspera
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838014
http://www.ncbi.nlm.nih.gov/pubmed/20097811
http://dx.doi.org/10.1128/AEM.01917-09
op_rights Copyright © 2010, American Society for Microbiology
op_doi https://doi.org/10.1128/AEM.01917-09
container_title Applied and Environmental Microbiology
container_volume 76
container_issue 6
container_start_page 1813
op_container_end_page 1821
_version_ 1766389090886877184