Shotgun Proteomics Analysis of Hibernating Arctic Ground Squirrels*
Mammalian hibernation involves complex mechanisms of metabolic reprogramming and tissue protection. Previous gene expression studies of hibernation have mainly focused on changes at the mRNA level. Large scale proteomics studies on hibernation have lagged behind largely because of the lack of an ade...
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ftpubmed:oai:pubmedcentral.nih.gov:2830842 2023-05-15T14:55:54+02:00 Shotgun Proteomics Analysis of Hibernating Arctic Ground Squirrels* Shao, Chunxuan Liu, Yuting Ruan, Hongqiang Li, Ying Wang, Haifang Kohl, Franziska Goropashnaya, Anna V. Fedorov, Vadim B. Zeng, Rong Barnes, Brian M. Yan, Jun 2010-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830842 http://www.ncbi.nlm.nih.gov/pubmed/19955082 https://doi.org/10.1074/mcp.M900260-MCP200 en eng The American Society for Biochemistry and Molecular Biology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830842 http://www.ncbi.nlm.nih.gov/pubmed/19955082 http://dx.doi.org/10.1074/mcp.M900260-MCP200 © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. Research Text 2010 ftpubmed https://doi.org/10.1074/mcp.M900260-MCP200 2013-09-02T22:03:02Z Mammalian hibernation involves complex mechanisms of metabolic reprogramming and tissue protection. Previous gene expression studies of hibernation have mainly focused on changes at the mRNA level. Large scale proteomics studies on hibernation have lagged behind largely because of the lack of an adequate protein database specific for hibernating species. We constructed a ground squirrel protein database for protein identification and used a label-free shotgun proteomics approach to analyze protein expression throughout the torpor-arousal cycle during hibernation in arctic ground squirrels (Urocitellus parryii). We identified more than 3,000 unique proteins from livers of arctic ground squirrels. Among them, 517 proteins showed significant differential expression comparing animals sampled after at least 8 days of continuous torpor (late torpid), within 5 h of a spontaneous arousal episode (early aroused), and 1–2 months after hibernation had ended (non-hibernating). Consistent with changes at the mRNA level shown in a previous study on the same tissue samples, proteins involved in glycolysis and fatty acid synthesis were significantly underexpressed at the protein level in both late torpid and early aroused animals compared with non-hibernating animals, whereas proteins involved in fatty acid catabolism were significantly overexpressed. On the other hand, when we compared late torpid and early aroused animals, there were discrepancies between mRNA and protein levels for a large number of genes. Proteins involved in protein translation and degradation, mRNA processing, and oxidative phosphorylation were significantly overexpressed in early aroused animals compared with late torpid animals, whereas no significant changes at the mRNA levels between these stages had been observed. Our results suggest that there is substantial post-transcriptional regulation of proteins during torpor-arousal cycles of hibernation. Text Arctic Urocitellus parryii PubMed Central (PMC) Arctic Molecular & Cellular Proteomics 9 2 313 326 |
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Research Shao, Chunxuan Liu, Yuting Ruan, Hongqiang Li, Ying Wang, Haifang Kohl, Franziska Goropashnaya, Anna V. Fedorov, Vadim B. Zeng, Rong Barnes, Brian M. Yan, Jun Shotgun Proteomics Analysis of Hibernating Arctic Ground Squirrels* |
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Research |
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Mammalian hibernation involves complex mechanisms of metabolic reprogramming and tissue protection. Previous gene expression studies of hibernation have mainly focused on changes at the mRNA level. Large scale proteomics studies on hibernation have lagged behind largely because of the lack of an adequate protein database specific for hibernating species. We constructed a ground squirrel protein database for protein identification and used a label-free shotgun proteomics approach to analyze protein expression throughout the torpor-arousal cycle during hibernation in arctic ground squirrels (Urocitellus parryii). We identified more than 3,000 unique proteins from livers of arctic ground squirrels. Among them, 517 proteins showed significant differential expression comparing animals sampled after at least 8 days of continuous torpor (late torpid), within 5 h of a spontaneous arousal episode (early aroused), and 1–2 months after hibernation had ended (non-hibernating). Consistent with changes at the mRNA level shown in a previous study on the same tissue samples, proteins involved in glycolysis and fatty acid synthesis were significantly underexpressed at the protein level in both late torpid and early aroused animals compared with non-hibernating animals, whereas proteins involved in fatty acid catabolism were significantly overexpressed. On the other hand, when we compared late torpid and early aroused animals, there were discrepancies between mRNA and protein levels for a large number of genes. Proteins involved in protein translation and degradation, mRNA processing, and oxidative phosphorylation were significantly overexpressed in early aroused animals compared with late torpid animals, whereas no significant changes at the mRNA levels between these stages had been observed. Our results suggest that there is substantial post-transcriptional regulation of proteins during torpor-arousal cycles of hibernation. |
format |
Text |
author |
Shao, Chunxuan Liu, Yuting Ruan, Hongqiang Li, Ying Wang, Haifang Kohl, Franziska Goropashnaya, Anna V. Fedorov, Vadim B. Zeng, Rong Barnes, Brian M. Yan, Jun |
author_facet |
Shao, Chunxuan Liu, Yuting Ruan, Hongqiang Li, Ying Wang, Haifang Kohl, Franziska Goropashnaya, Anna V. Fedorov, Vadim B. Zeng, Rong Barnes, Brian M. Yan, Jun |
author_sort |
Shao, Chunxuan |
title |
Shotgun Proteomics Analysis of Hibernating Arctic Ground Squirrels* |
title_short |
Shotgun Proteomics Analysis of Hibernating Arctic Ground Squirrels* |
title_full |
Shotgun Proteomics Analysis of Hibernating Arctic Ground Squirrels* |
title_fullStr |
Shotgun Proteomics Analysis of Hibernating Arctic Ground Squirrels* |
title_full_unstemmed |
Shotgun Proteomics Analysis of Hibernating Arctic Ground Squirrels* |
title_sort |
shotgun proteomics analysis of hibernating arctic ground squirrels* |
publisher |
The American Society for Biochemistry and Molecular Biology |
publishDate |
2010 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830842 http://www.ncbi.nlm.nih.gov/pubmed/19955082 https://doi.org/10.1074/mcp.M900260-MCP200 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Urocitellus parryii |
genre_facet |
Arctic Urocitellus parryii |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830842 http://www.ncbi.nlm.nih.gov/pubmed/19955082 http://dx.doi.org/10.1074/mcp.M900260-MCP200 |
op_rights |
© 2010 by The American Society for Biochemistry and Molecular Biology, Inc. |
op_doi |
https://doi.org/10.1074/mcp.M900260-MCP200 |
container_title |
Molecular & Cellular Proteomics |
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9 |
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2 |
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313 |
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326 |
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1766327907387441152 |