Carbonic acid buffer species measured in real time with an intracellular microelectrode array
Carbonic acid buffer anions, HCO3−andCO32−, play an instrumental role in a host of vital processes in animal cells and tissues. Yet study of carbonic acid buffer species is hampered because no means are available to simultaneously monitor them at a cellular level in a rapid and dynamic fashion. An i...
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ftpubmed:oai:pubmedcentral.nih.gov:2826694 2023-05-15T15:52:27+02:00 Carbonic acid buffer species measured in real time with an intracellular microelectrode array Wietasch, Kristina Kraig, Richard P. 1991-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826694 http://www.ncbi.nlm.nih.gov/pubmed/1653544 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826694 http://www.ncbi.nlm.nih.gov/pubmed/1653544 Copyright © 1991 the American Physiological Society Article Text 1991 ftpubmed 2013-09-02T21:48:07Z Carbonic acid buffer anions, HCO3−andCO32−, play an instrumental role in a host of vital processes in animal cells and tissues. Yet study of carbonic acid buffer species is hampered because no means are available to simultaneously monitor them at a cellular level in a rapid and dynamic fashion. An ion-selective cocktail, previously reported to measure changes in bicarbonate activity (αHCO3−), was instead shown to be principally selective for αCO32−. Ion-selective micropipettes (ISMs) based on this exchanger and consisting of a 3:1:6 (volume) mixture of tri-n-octylpropylammonium chloride, 1-octanol, and trifluoroacetyl-p-butylbenzene showed no significant interference from bicarbonate, chloride, phosphate, ascorbate, lactate, glutamate, acetate, or hydroxyl ions at concentrations expected in vivo. Intracellular and triple-barrel ISMs, consisting of a CO32−-sensitive, pH-sensitive, and reference barrel, were fabricated. Skeletal muscle cells (n = 17) were penetrated in vivo and showed values of 74 ± 7 mV for membrane potential, 6.94 ± 0.09 pHi, and 11 ± 5 µM intracellular αCO32−, from which intracellular αHCO3− of 25 ± 10 mM and CO2 tension of 120 ± 55 Torr were calculated. All ion measurements reached a new steady state in 9 ± 2 s after cell penetration. Thus measurements of intracellular αCO32− and pH and associated levels of αHCO3 and CO2 tension can be determined in biological tissues and cells with a spatial and temporal resolution previously unattainable. Text Carbonic acid PubMed Central (PMC) |
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Article Wietasch, Kristina Kraig, Richard P. Carbonic acid buffer species measured in real time with an intracellular microelectrode array |
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Article |
description |
Carbonic acid buffer anions, HCO3−andCO32−, play an instrumental role in a host of vital processes in animal cells and tissues. Yet study of carbonic acid buffer species is hampered because no means are available to simultaneously monitor them at a cellular level in a rapid and dynamic fashion. An ion-selective cocktail, previously reported to measure changes in bicarbonate activity (αHCO3−), was instead shown to be principally selective for αCO32−. Ion-selective micropipettes (ISMs) based on this exchanger and consisting of a 3:1:6 (volume) mixture of tri-n-octylpropylammonium chloride, 1-octanol, and trifluoroacetyl-p-butylbenzene showed no significant interference from bicarbonate, chloride, phosphate, ascorbate, lactate, glutamate, acetate, or hydroxyl ions at concentrations expected in vivo. Intracellular and triple-barrel ISMs, consisting of a CO32−-sensitive, pH-sensitive, and reference barrel, were fabricated. Skeletal muscle cells (n = 17) were penetrated in vivo and showed values of 74 ± 7 mV for membrane potential, 6.94 ± 0.09 pHi, and 11 ± 5 µM intracellular αCO32−, from which intracellular αHCO3− of 25 ± 10 mM and CO2 tension of 120 ± 55 Torr were calculated. All ion measurements reached a new steady state in 9 ± 2 s after cell penetration. Thus measurements of intracellular αCO32− and pH and associated levels of αHCO3 and CO2 tension can be determined in biological tissues and cells with a spatial and temporal resolution previously unattainable. |
format |
Text |
author |
Wietasch, Kristina Kraig, Richard P. |
author_facet |
Wietasch, Kristina Kraig, Richard P. |
author_sort |
Wietasch, Kristina |
title |
Carbonic acid buffer species measured in real time with an intracellular microelectrode array |
title_short |
Carbonic acid buffer species measured in real time with an intracellular microelectrode array |
title_full |
Carbonic acid buffer species measured in real time with an intracellular microelectrode array |
title_fullStr |
Carbonic acid buffer species measured in real time with an intracellular microelectrode array |
title_full_unstemmed |
Carbonic acid buffer species measured in real time with an intracellular microelectrode array |
title_sort |
carbonic acid buffer species measured in real time with an intracellular microelectrode array |
publishDate |
1991 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826694 http://www.ncbi.nlm.nih.gov/pubmed/1653544 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826694 http://www.ncbi.nlm.nih.gov/pubmed/1653544 |
op_rights |
Copyright © 1991 the American Physiological Society |
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